Write a short discussion based on the result above (gel electrophoresis)
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Write a short discussion based on the result above (gel electrophoresis)
Step by step
Solved in 3 steps with 1 images
- Copy and paste the link below and watch the video on Youtube https://www.youtube.com/watch?v=8RBs0Ghg_48 Answer the following Questions: 1. What are the chemicals and materials used in gel electrophoresis? 2. Draw a schematic diagram of a gel electrophoresis set-up 3. Describe the procedure in doing a gel electrophoresis experiment. Why is there a need for a leveling bubble/leveler? What is the use of the rubber dam? 4. What is the use of ethidium bromide and why must you wear gloves when you handle it? 5. What makes the DNA fragment move towards the positive plate? 6. What is the purpose of glycerol in the sample buffer? 7. What is the use of a DNA ladder? 8. What will happen when you increase the voltage of the set-up? 9. Can gel electrophoresis be used to separate amino acids? If so, how is it done?What is gel electrophoresis and why is it important? Write in detail.Give a detailed introduction to gel electrophoresis practical in your own words.
- for gel electropertisis, what would be an appropriate caption for the tableWhat are the different applications of Gel Electrophoresis? explain each applicationsImage 1 shows raw data of gel electrophoresis. Label/annotate image 1 (lanes, ladder sizes, etc). Explain what your seeing in the gel. Use the following information and picture 2 to assist in labelling. The purpose of this gel electrophorsis is to ensure that your GOI, FAP257, is in each BAC. Protocol that was done for Gel electrophoresis: -Place tray with gel into gel box -Fill gel box with IX TAE until the gel is completely submerged -Remove the comb for wells -Load 10ul of the 1kb Gene Ruler ladder (well 1) -Contains DNA ladder, 6X TriTrack DNA, Loading Dye, and Deionized water -Load other wells -Well 2: Control -Well 3: BAC- 15M5 -Well 4: BAC-39K10 -Well 5: BAC-27N17 -Run the gel at 100V for 30 minutes or until the dye front has migrated 2/3 down the gel
- Define the term gel electrophoresis.In a report, describe what gel Electrophoresis is and its principles3) Define gel electrophoresis, including its theory and application. Describe the steps of running gel electrophoresis using the following image. More detailed reading: https://www.sciencedirect.com/topics/medicine-and-dentistry/agar-gel-electrophoresis POWER SUPPLY CATHODE ELECTROPHORETIC BUFFER ANODE WELL SAMPLE AGAROSE GEL POWER SUPPLY CATHODE ANOCE HIGH MOLECULAR WEIGHT SPECIES LOW MOLECULAR WEIGHT ANALYTES