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HYDROLYSIS OF DNA A. To the fibrous mass, add 10 ml of 10% H,SO, in a beaker. Boil gently or 5 minutes. Perform the following test: 1. Inorganic phosphate – to 1 ml NH,OH, add 1 ml filtrate. Acidify by placing a small piece of blue litmus paper using 10% HNO, until the litmus paper changes to red. Then add 2 ml ammonium molybdate. Boil the solution and allow to stand for a few minutes. Note color of ppt. 2. Purines - to 2 ml filtrate add 3 ml of 3M NH,OH. Add 2-3 drops of 0.1M AgNO, solution to it. Note color of precipitate. 3. Deoxyribose – to 1 ml filtrate add 1 ml of diphenylamine reagent. Heat for 10 mins in a boiling water bath. Cool and note color produced. Hyrolysis of DNA Test Results 1. Inorganic Phosphate 2. Purines 3. Deoxyribose

HYDROLYSIS OF DNA A. To the fibrous mass, add 10 ml of 10% H,SO, in a beaker. Boil gently or 5 minutes. Perform the following test: 1. Inorganic phosphate – to 1 ml NH,OH, add 1 ml filtrate. Acidify by placing a small piece of blue litmus paper using 10% HNO, until the litmus paper changes to red. Then add 2 ml ammonium molybdate. Boil the solution and allow to stand for a few minutes. Note color of ppt. 2. Purines - to 2 ml filtrate add 3 ml of 3M NH,OH. Add 2-3 drops of 0.1M AgNO, solution to it. Note color of precipitate. 3. Deoxyribose – to 1 ml filtrate add 1 ml of diphenylamine reagent. Heat for 10 mins in a boiling water bath. Cool and note color produced. Hyrolysis of DNA Test Results 1. Inorganic Phosphate 2. Purines 3. Deoxyribose

Biochemistry
Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
Problem 1P
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HYDROLYSIS OF DNA A. To the fibrous mass, add 10 ml of 10% H,SO, in a beaker. Boil gently or 5 minutes. Perform the following test: 1. Inorganic phosphate – to 1 ml NH,OH, add 1 ml filtrate. Acidify by placing a small piece of blue litmus paper using 10% HNO, until the litmus paper changes to red. Then add 2 ml ammonium molybdate. Boil the solution and allow to stand for a few minutes. Note color of ppt. 2. Purines - to 2 ml filtrate add 3 ml of 3M NH,OH. Add 2-3 drops of 0.1M AgNO, solution to it. Note color of precipitate. 3. Deoxyribose – to 1 ml filtrate add 1 ml of diphenylamine reagent. Heat for 10 mins in a boiling water bath. Cool and note color produced.
Transcribed Image Text:HYDROLYSIS OF DNA A. To the fibrous mass, add 10 ml of 10% H,SO, in a beaker. Boil gently or 5 minutes. Perform the following test: 1. Inorganic phosphate – to 1 ml NH,OH, add 1 ml filtrate. Acidify by placing a small piece of blue litmus paper using 10% HNO, until the litmus paper changes to red. Then add 2 ml ammonium molybdate. Boil the solution and allow to stand for a few minutes. Note color of ppt. 2. Purines - to 2 ml filtrate add 3 ml of 3M NH,OH. Add 2-3 drops of 0.1M AgNO, solution to it. Note color of precipitate. 3. Deoxyribose – to 1 ml filtrate add 1 ml of diphenylamine reagent. Heat for 10 mins in a boiling water bath. Cool and note color produced.
Hyrolysis of DNA Test Results 1. Inorganic Phosphate 2. Purines 3. Deoxyribose
Transcribed Image Text:Hyrolysis of DNA Test Results 1. Inorganic Phosphate 2. Purines 3. Deoxyribose
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