You have a 3.5% (w/v) aqueous solution of protein. You want to make 2 mL of 0.8%(w/v) protein solution. What volume of the 3.5% solution do you need?457 µl45.7 µl3.5 mLO 350 µLO 1.6 mL

Weight by volume concentration means the amount of the substance in grams dissolved in 100ml of the…

Answered: You have a 3.5% (w/v) aqueous solution…

Biochemistry

9th Edition

ISBN:9781319114671

Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Chapter1: Biochemistry: An Evolving Science

Section: Chapter Questions

Problem 1P

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Similar questions

A 1.143 g sample contains only vitamin C (C,H, 0,) and sucralose (C,H,Cl, O,). When the sample is dissolved in water to a total volume of 31.7 mL, the osmotic pressure of the solution is 3.91 atm at 285 K. What is the mass percent of vitamin C and sucralose in the sample? vitamin C: sucralose: %
You are given a protein solution with a concentration of 0.15 mg/ml. We need 10 μg for an experiment. What volume of the protein solution do we need?
In protein electrophoresis, what reagent present in the sample buffer is used to eliminate differences in the charge densities of proteins? Tris-HCL ph 8.8 2-mecraptothanol SDS(sodium dodecyl sulfate) Tris-HCL PH 6.8
A stock solution contains 0.2 mg/mL protein. From this stock solution, 179 uL is diluted with 1.1 mL buffer. What is the concentration of protein in this dilution?
A purified protein fraction has a total sample volume of 450 µL. The sample has a corrected A280 of 0.244, and the blank corrected A280 was 0.077. (Both values were measured with a path length of 1.00 cm.) If 5.50 µL of the sample was used in a reaction, calculate the mass of protein in the reaction (in µg).
If equal portions of tretinoin gel, 0.1% w/w and 0.04% w/w are combined, what would be the resultant percentage strength?
How many mL of 10 x stock solution would you need to make 118 mL of 1x PBS? Report your answer to 1 decimal place.
Four lab groups completed the experiment listed in the lab diluting lul of FCF dye into 1ml of water. The A625 values are listed below. For brilliant blue FCF, ɛ =97000 M-1cm–1 MW = 792.8 g/mol %3D Group # A625 % solution (in original stock sample) Percent ror (True value of Stock=0.2%) 1 0.244 0.199 0.5 0.209 0.170 15 3 0.306 0.250 25 4 0.187 0.153 0.235 a. Which group was more accurate? b. What are possible reasons group 3 had a much higher Absorbance than the others? c. What are possible reasons group 4 had a much lower Absorbance than the others?
A purified protein fraction has a total sample volume of 360 µL. The sample has a corrected A280 of 0.484, and the blank corrected A280 was 0.052. (Both values were measured with a path length of 1.00 cm.) If 5.00 µL of the sample was used in a reaction, calculate the mass of protein in the reaction (in µg).
In quantitative determination of protein using spectrophotometer how can I plot the absorbance vs wavelength for the proteins given the transmittance values?

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You have a 3.5% (w/v) aqueous solution of protein. You want to make 2 mL of 0.8% (w/v) protein solution. What volume of the 3.5% solution do you need? 457 µl 45.7 µl 3.5 mL O 350 µL O 1.6 mL