You have a 3.5% (w/v) aqueous solution of protein. You want to make 2 mL of 0.8%(w/v) protein solution. What volume of the 3.5% solution do you need?457 µl45.7 µl3.5 mLO 350 µLO 1.6 mL

Weight by volume concentration means the amount of the substance in grams dissolved in 100ml of the…

Answered: You have a 3.5% (w/v) aqueous solution…

Biochemistry

9th Edition

ISBN:9781319114671

Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Chapter1: Biochemistry: An Evolving Science

Section: Chapter Questions

Problem 1P

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A 1.143 g sample contains only vitamin C (C,H, 0,) and sucralose (C,H,Cl, O,). When the sample is dissolved in water to a total volume of 31.7 mL, the osmotic pressure of the solution is 3.91 atm at 285 K. What is the mass percent of vitamin C and sucralose in the sample? vitamin C: sucralose: %
You are given a protein solution with a concentration of 0.15 mg/ml. We need 10 μg for an experiment. What volume of the protein solution do we need?
Why are we using this chemicals?(Determination of solubility and soaping number of lipids) Materials: *Fats and oils*Oil solvent (Equal volume 95% ethanol and ether)*0.5 M alcoholic KOH solution*0.05% phenolphthalein solution*0.5 M HCl solution Procedures: Determination of Saponification Number 1) Dissolve 1 g (or 2 mL) of oil in 3 mL of oil solvent and add 25 mL of KOH solution. Put a few boiling stones and boil in the water bath by mixing for 30 minutes.2) Add 3 drops of phenolphthalein to the saponified and brought to room temperature solution and titrate with HCl.3)Titrate 25 mL of KOH solution containing 2 mL of water with HCl for a blank test. Solubility of lipids 1) Record the physical properties of all oils before starting the experiment. Then observe the solubility of the oils in water and organic solvents. Discuss the difference.2) Add 1 mL of water to the oil solutions in ethyl alcohol. Mix vigorously and record your observations in the first second and after 5 minutes.
In protein electrophoresis, what reagent present in the sample buffer is used to eliminate differences in the charge densities of proteins? Tris-HCL ph 8.8 2-mecraptothanol SDS(sodium dodecyl sulfate) Tris-HCL PH 6.8
A stock solution contains 0.2 mg/mL protein. From this stock solution, 179 uL is diluted with 1.1 mL buffer. What is the concentration of protein in this dilution?
A purified protein fraction has a total sample volume of 450 µL. The sample has a corrected A280 of 0.244, and the blank corrected A280 was 0.077. (Both values were measured with a path length of 1.00 cm.) If 5.50 µL of the sample was used in a reaction, calculate the mass of protein in the reaction (in µg).
If equal portions of tretinoin gel, 0.1% w/w and 0.04% w/w are combined, what would be the resultant percentage strength?
How many mL of 10 x stock solution would you need to make 118 mL of 1x PBS? Report your answer to 1 decimal place.
Four lab groups completed the experiment listed in the lab diluting lul of FCF dye into 1ml of water. The A625 values are listed below. For brilliant blue FCF, ɛ =97000 M-1cm–1 MW = 792.8 g/mol %3D Group # A625 % solution (in original stock sample) Percent ror (True value of Stock=0.2%) 1 0.244 0.199 0.5 0.209 0.170 15 3 0.306 0.250 25 4 0.187 0.153 0.235 a. Which group was more accurate? b. What are possible reasons group 3 had a much higher Absorbance than the others? c. What are possible reasons group 4 had a much lower Absorbance than the others?
Answer the following with complete solutions
8. What mass of solute is needed to prepare each of 1.00 L of 0.125 M K2SO4 solutions? (2 Points) 21.8 g K2SO4 218.0 g K2SO4 2.816 g K2SO4 28.16 g K2SO4
You are a graduate student who is trying to conduct an experiment. For this experiment, you need to create a 500 micromolar solution of Solution X. You have a stock solution of Solution X 500 millimolars. Your final volume needs to be 10 milliliters. How much of the stock solution do you need to add and how much water needs to be added in order to create a 50 micromolar solution of Solution X? O 1,000 microliters of X and 9 milliliters of water O 1 microliters of X and 9.999 milliliters of water 0.01 microliters of X and 9.99 milliliters of water 10 microliters of X and 9.99 milliliters of water

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You have a 3.5% (w/v) aqueous solution of protein. You want to make 2 mL of 0.8% (w/v) protein solution. What volume of the 3.5% solution do you need? 457 µl 45.7 µl 3.5 mL O 350 µL O 1.6 mL