QUESTION 22MstdigestonHb*I Hb HDI HD Hb*I HbE 1.35 kbE 1.16 kbSouthern blotThe figure above is a diagram of a Southern blot using a probe for the hemoglobin Hb gene. The blot shows a restriction fragment polymorphism (RFLP)related to the sickling trait (Hbs) in an Mst II restriction enzyme digest. Whích of the following statements about the location of the probe iscorrect?O A The Hbs mutation destroys an Mst II restriction site located within the probe hybridization region.O B. The Hbs mutation creates a new Mst II restriction site located within the probe hybridization region.O C. The probe hybridization region is completely contained on a 0.2 kb MstII restriction fragment.O D. The probe hybridization region is completely contained on a 1.15 kb MstII restriction fragment.O E. None of the above is correct.

As indicated in the RFLP and restriction digestion analysis of the HbA and HbS gene there is a…

Answered: QUESTION 22 Mst digeston Hb*I Hb HDI HD…

Biochemistry

9th Edition

ISBN:9781319114671

Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Chapter1: Biochemistry: An Evolving Science

Section: Chapter Questions

Problem 1P

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Question 11 Which polymerase is responsible for transcribing RNA from a single transcription unit, generating a large transcript that is then processed to yield three types of ribosomal RNA Question 11 options: RNA Polymerase I RNA Polymerase II RNA Polymerase III DNA Polymerase Question 12 RNA splicing requires recognition of which nucleotide sequences at the boundaries of transcribed exons and introns (splice junctions)? Question 12 options: GT–AG TG-CA CT-CG CpG Question 13 Following are the three consensus DNA sequences in introns of complex eukaryotes except: Question 13 options: Splice donor site Splice acceptor site Branch site Initiation site
Question 5 Review Figure 21.6 types of DNA sequences in the human genome. The lowest percentage of human genome O repetitive DNA related to transposable elements and related sequences (L1 sequences and Alu elements) O repetitive DNA unrelated to transposable elements (simple sequence and large-segment duplications O unique noncoding DNA O introns O exons O regulatory sequences
QUESTION 19 Place the following steps in the expression of the lac operon in the order in which each occurs for the first time after a cell is induced. v A phosphodiester bond is formed between two ribonucleotides. - A peptide bond is formed between the first two amino acids in beta-galactosidase (lac Z). Sigma protein binds to a specific DNA sequence. RNA polymerase ends transcription of the lacA gene. v A protein leaves an operator DNA sequence. v A ribosomal RNA binds to a complementary sequence in mRNA.
Question 6-10: Choose the enzyme and match it to its function. Bubble the correct letter on the Zipgrade answer sheet. Primase DNA Polymerase Ligase Helicase Topoisomerase/gyrase а. b. с. d. е. 6. This enzyme starts the replication process with a small strand of RNA that is later replaced. 7. This enzyme removes primer and starts adding DNA. 8. This enzyme joins Okazaki Fragments. 9. This enzyme unwinds the original strands of DNA so they can act as templates. 10. This enzyme prevents supercoiling .
QUESTION 22 During the search for the Cystic Fibrosis (CF) gene the investigators used various criteria to conclude that they had arrived at a segment that represented a gene. Which of the properties below was among the criteria used by the investigators? O A. the presence of sequence palindromes O B. gene-specific restriction maps O C. interspecies sequence conservation O D. polyadenylation signals O E. the presence of AT-rich islands QUESTION 23 A feature common to activation of the myc oncogene by both the Avian Leukosis virus and the chromosomal 8:14 translocation is: O A. loss of the 1st myc exon (E1); initiation of transcription from a start site within the myc intron O B. Initiation of transcription from a strong promoter upstream from the first myc exon (E1) O C.A fusion transcript between an active upstream gene and the three myc exons O D.A DNA deletion that eliminates the transcriptional termination signal from an active upstream gene. O E. Recombination between the myc…
Question 6 If the plasmid were completely digested with the two restriction enzymesBamHI andHgiEII, the resulting fragments would be this large: Clal (24) ... EcoRI (4359) Hindi (29) EcoRV (187) Apali (4033) BamHI (375) Pstl (3611) HaiEll (3056) 3400 3200 3000 Apall (2787) 2800 Sspl (4170) a-lactamase gene 3800 400 Afull A) 3056, 2295, 375 base pairs long B) 1980, 1620 and 761 base pairs long C) 1920, 1305, 761 and 375 base pairs long D 1920, 1680, 761 base pairs long 2600 pBR322 4361bp 2200 2400 Hai Ell (2295) 200 400 600 tetracycline resista 800 1800 1600 2000 rop Pvull (2066) 1000 Sphi (566) 004 1400 Sall (651)
Question 4 "In this mutation, the AGC is transcribed into UAG. " O missense mutation O nonsense mutation O silent mutation O frameshift mutation
QUESTION 17 OL NEere interested in generating a PCR amplicon including the bracketed sequence below. Which of the following sequences would be canen hybridizing (annealing) with the target AND would also serve to generate a copy of the bracketed region of interest? 5'-AATCGT[AGCAGCAGCAGTGGCT]A AGCT-3 3' -TTAGCA[TC GTC GTC GTC ACC G A] TTCG A - 5' 3-TTAGC-S S-AATCG-3 OSAAGCT-3 5-AGCTT-3 5-GCTAA-3 5-TCGAA-3 QUESTION 18 Vhich of the following is/are true regarding the enzvme PRIMASE? Save and Submit to save and submit. Click Save All Answers to save all answers.
QUESTION 7 The recognition sites for the restriction enzymes BamHI, Xbal, and BglII are G^GATCC, T^CTAGA, and A^GATCT, respectively. Which enzymes leave compatible ends that can be ligated together? O A. All of these enzymes leave ends that are compatible with ends generated by the others. O B. None of the enzymes produce compatible ends. C. Only BamHI and BglII fragments are compatible. O D. Only BamHI and Xbal fragments are compatible. O E. Only BglII and Xbal fragments are compatible.
QUESTION 45 Gene edits can be made to Eukaryotic DNA by using a complex composed of: Progenitor cell and 4 master regulatory genes Cyclic AMP and CRP protein Ubiquitin protein and CRISPR Guide RNA and Cas 9 protein Histone proteins and methyl groups, QUESTION 46 Click Saye and
Question 44 Plasmids to become effective vectors and cloning really successful, they must be double stranded and generate blunt ends after restriction enzyme digestion. A True B) False
Question 1: The restriction enzyme Sau3A recognizes the sequence 5'-/GATC-3' and cleaves on the 5' side of the G as indicated by the slash. Since the top and bottom strands of this restriction site are identical in the 5'-3' direction, only one strand of the site needs to be shown. The single-stranded ends produced by Sau3A cleavage are identical to those produced by BamHI, which recognizes the sequence 5'-G/GATCC-3' and cuts at the location of the slash. This means that an end cut by one enzyme can be ligated onto an end cut by the other enzyme by DNA Ligase. Given this information, answer the following questions: A) Write out both strands of the recognition sequences for BamHI and Sau3A, show what they would look like after they are cut, and what the DNA would look like after you ligate a BamHI site to a Sau3A site. Make sure that it is easy to follow the individual pieces of DNA. You may want to use two different colors to keep them separate. B) What fraction of BamHI sites can be…

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