QUESTION 22MstdigestonHb*I Hb HDI HD Hb*I HbE 1.35 kbE 1.16 kbSouthern blotThe figure above is a diagram of a Southern blot using a probe for the hemoglobin Hb gene. The blot shows a restriction fragment polymorphism (RFLP)related to the sickling trait (Hbs) in an Mst II restriction enzyme digest. Whích of the following statements about the location of the probe iscorrect?O A The Hbs mutation destroys an Mst II restriction site located within the probe hybridization region.O B. The Hbs mutation creates a new Mst II restriction site located within the probe hybridization region.O C. The probe hybridization region is completely contained on a 0.2 kb MstII restriction fragment.O D. The probe hybridization region is completely contained on a 1.15 kb MstII restriction fragment.O E. None of the above is correct.

As indicated in the RFLP and restriction digestion analysis of the HbA and HbS gene there is a…

Answered: QUESTION 22 Mst digeston Hb*I Hb HDI HD…

Biochemistry

9th Edition

ISBN:9781319114671

Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Chapter1: Biochemistry: An Evolving Science

Section: Chapter Questions

Problem 1P

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QUESTION 48 Identify the best match between the mutation description and term. a. Synonymous mutation: has the potential to cause large changes in transcription and subsequence amino acid sequence due to reading frameshifts b. Nonsense mutation: causes a drastic change in phenotype because the change causes a premature stop in the amino acid sequence c. Indel: a change in the DNA that changes the codon code from one amino acid to another amino acid d. Missense mutation: results in a change in single nucleotide from a purine to another purine or a pyrimidine to another pyrimidine but does not change the amino acid sequence
Question 5 In order for PCR (polymerase chain reaction) to be possible, we need to add to the reaction: a DNA polymerase that can work at high temperature, e.g., 72C. MRNA translation factors marker DNA « Previous
QUESTION 2 The SARS-COV-2 pandemic resulted in the desire to design ELISA assays to either detect antibodies against SARS-CoV-2 protein (previous infection), or detect viral protein (current infection). You plan to express the mutant spike protein variant using the plasmid pictured below as your template. Here's the sequence you want to put in its place: https://www.ncbi.nlm.nih.gov/nuccore/AY429073.1?report=fasta, this is the DNA sequence you're going to buy as a gene block! But you need to add a couple things to it first so it'll get into the plasmid. To do so, you're going to need to remove the current insert (represented by the yellow arrow beginning at position 833-4867). Question: What restriction sites are you going to add at the ends of the gene? Answer can be in nucleotides or the enzyme name. For the first blank fill in the site name/sequence you'd add at the beginning of the gene (5' / C-terminus end) and for the second blank fill in the sequence/site name you'd add at the…
QUESTION 22 Choose all the experiment(s) from the list below that demonstrated that RNA is involved in a transfer step between DNA and protein. O A. pulse chase experiments using radioactive deoxyribose O B. Brenner and Crick's characterization of frameshift suppressor mutations O C. analysis of the burst of RNA synthesis after bacteriophage infection of E. coli O D. pulse chase experiments using radioactive uracil bases O E. the Meselson Stahl experiment
Question 18 Match Column A with Column B. transports amino acids to the site of protein synthesis A. operator B. rRNA has anticodon loop C. IRNA v directs amino acid sequence of proteins D. MRNA contains the Shine-Delgarno sequence E. most type of RNA v synthesized in the eukaryote nucleus F. promoter v found inside the ribosome v region where the RNA binds to the DNA template region where the activator protein binds to the DNA template
Question 44 The tri snRNP particle O contains the snRNPs U4,U5 and U6 comes after the binding of U2 brings the branch point and the 5' splice site together O all the above A Click Submit to complete this assessment. Mac DO00
Question 4. You are analyzing cells that you have grown on coverslips and fixed. What method would you use to determine where in the cell a protein is located? A. Western blot B. SDS-PAGE C. Microarray D. Immnocytochemstry E. Single cell RNA sequencing
QUESTION 20 Match the lettered activities to the following enzymes. Remember that some enzymes have multiple activities. A. 5' to 3' polymerase activity B. 3' to 5' DNA polymerase activity C. RNA-dependent DNA polymerase activity D. DNA dependent DNA polymerase activity E. 3' to 5' exonuclease activity F. 5' to 3' exonuclease activity DNA polymerase II DNA polymerase I telomerase
question 3
QUESTION 5 Based on the plasmid shown, what will you have to use to to distinguish and isolate the bacteria that successfully took up the plasmid? O Lactose O Tetracycline O Ampicillin O UV light to identify the glowing colonies O Xgal
question 4

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