9. Compare the effects of metals, temperature, and pH on the catalase enzyme. a. Did any of these variables enhance the enzyme's activity? How do you know? b. Did any of these variables inhibit the enzyme's activity? How do you know? C. What were the optimum conditions for catalase enzyme? Consider that the catalase enzyme is present in almost all organisms (including in human livers) in order to protect the cells from reactive oxygen species (ROS) such as H2O2. Do these optimum conditions make sense based on where the enzyme is found? 7. Make a data table for the experiments/trials. It should include reaction conditions volumes of reagents used, O2 volume produced (with correct sig figs), and observation Tor all trials. If you have made a figure displaying your results, please attach that as well | temperature Vol of Hio Vol of polato un time Oz generated 1. o'c 5ml 2ml 2. 2. 1 mL 2. 8.5 mb 37°C 4 1,8 mb 8. Analyzing your results: a. What effect does your variable have on the activity of the potato enżyme? Use numerical data to describe the how the activity changes. b. Why do you think you saw this effect? c. What specifically happened to the enzyme to cause this effect? What conclusions can you draw from the change or lack of change to the activity of the potato enzyme? d. Summarize your conclusions including numerical data on the board for your system.
9. Compare the effects of metals, temperature, and pH on the catalase enzyme. a. Did any of these variables enhance the enzyme's activity? How do you know? b. Did any of these variables inhibit the enzyme's activity? How do you know? C. What were the optimum conditions for catalase enzyme? Consider that the catalase enzyme is present in almost all organisms (including in human livers) in order to protect the cells from reactive oxygen species (ROS) such as H2O2. Do these optimum conditions make sense based on where the enzyme is found? 7. Make a data table for the experiments/trials. It should include reaction conditions volumes of reagents used, O2 volume produced (with correct sig figs), and observation Tor all trials. If you have made a figure displaying your results, please attach that as well | temperature Vol of Hio Vol of polato un time Oz generated 1. o'c 5ml 2ml 2. 2. 1 mL 2. 8.5 mb 37°C 4 1,8 mb 8. Analyzing your results: a. What effect does your variable have on the activity of the potato enżyme? Use numerical data to describe the how the activity changes. b. Why do you think you saw this effect? c. What specifically happened to the enzyme to cause this effect? What conclusions can you draw from the change or lack of change to the activity of the potato enzyme? d. Summarize your conclusions including numerical data on the board for your system.
Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
Problem 1P
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Transcribed Image Text:9. Compare the effects of metals, temperature, and pH on the catalase enzyme.
a. Did any of these variables enhance the enzyme's activity? How do you know?
b. Did any of these variables inhibit the enzyme's activity? How do you know?
C. What were the optimum conditions for catalase enzyme? Consider that the
catalase enzyme is present in almost all organisms (including in human livers) in
order to protect the cells from reactive oxygen species (ROS) such as H2O2. Do
these optimum conditions make sense based on where the enzyme is found?
Transcribed Image Text:7. Make a data table for the experiments/trials. It should include reaction conditions
volumes of reagents used, O2 volume produced (with correct sig figs), and observation
Tor all trials. If you have made a figure displaying your results, please attach that as well
| temperature
Vol of Hio Vol of polato un time
Oz generated
1.
o'c
5ml
2ml
2.
2.
1 mL
2.
8.5 mb
37°C
4
1,8 mb
8. Analyzing your results:
a. What effect does your variable have on the activity of the potato enżyme? Use
numerical data to describe the how the activity changes.
b. Why do you think you saw this effect?
c. What specifically happened to the enzyme to cause this effect? What
conclusions can you draw from the change or lack of change to the activity of the
potato enzyme?
d. Summarize your conclusions including numerical data on the board for your
system.
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