Besides using A. tumefaciencs in the generation of transgenic plants, elaborate on the THREE (3) methods to introduce genc of interest into a plant cell.

Q: Targeting vectors use homologoues recombination to insert transgenes at specific chosen locations in…

A: Gene targeting by homologous recombination involves exchange of genetic material between genomic…

Q: T. Miyake and M. Demerec examined proline-requiring mutations in the bacterium Salmonella…

A: In transduction process, the closer the genes are present on a DNA, the more possibility they have…

Q: Hfr strains chosen for use in conjugation studies have the locus that controls antibiotic or phage…

A: Introduction :-  Most of the bacteria have plasmid , as the extra Chromosomal and self-replicating…

Q: Describe at least five (05) different types of commercially available vectors for cloning with size…

A: Cloning vectors are small DNA parts that are stably maintained in an organism. The foreign fragment…

Q: What is the molecular characteristic and phenotype of the silent mutation in multidrug resisitent…

A: Silent mutation are the mutations in DNA which do not have an phenotypic effect on the organism.…

Q: How to do linear Restriction Mapping of gene fragments, including a combination of three enzymes?

A: Bacteria contain restriction enzymes. To destroy viruses, bacteria use restriction enzymes, which…

Q: Clearly and neatly diagram an image of the agarose gel indicating the fragments that would be…

A: Electrophoresis It refers to the technique in which the fragments of molecules of DNA, RNA, or…

Q: Explain how bacterial conjugation has been extensively used in determining the complementing gene…

A: Bacterial conjugation is one of the most common ways for genetic information to spread between…

Q: You are attempting to prepare a single gene knockout library using the pRL27 transposon system. You…

A: * Given that prepared a single gene knockout library using the pRL27 transposon system. *Then grows…

Q: What is the role of sex pili during conjugation?

A: When genetic information is transferred from donor cell to recipient cell with the help of a bridge…

Q: Consider the following types of cells: F+, F-, Hfr, and F’ cells. Which of these four types of cells…

A:

Q: What does an improved variety of transgenic basmati rice contains?

A: Alteration of genetics: - It is a process through which the desired characters are incorporated in…

Q: Explain how viral vectors can be used for gene therapy, and provide two examples of commonly used…

A: The two methods of gene transfer used in gene therapy are: The viral-mediated gene transfer…

Q: Describe the method of pronuclear injection to generate transgenic mice?

A: Transgenic mice are produced by putting DNA in their genome from another source. There are a number…

Q: What is the significance of palindromic Restriction Endonuclease recognition and sequence overhangs…

A: Restriction endonucleases enzyme is also known as molecular scissors, which helps in cleavage of DNA…

Q: Discuss how to transform the yeast vector, YEP containing your gene of interest into the host cell.

A: To insert a set of desirable foreign genes into the host genome for replication and expression, a…

Q: Can you suggest how we could have determined whether Bill’s aunts were carriers of the mutant BTK…

A: The Bruton tyrosine kinase is important for the growth and maturation of B cells by some change and…

Q: What is o A vector derived from the tumor-inducing (Ti) plasmid of the bacterium Agrobacterium…

A: In recombinant technology, vectors are the DNA molecules that are used as a vehicle which carry…

Q: Besides cloning vectors, there are also expression vectors. What is the main differenc between these…

A: cloning vector is a small piece of DNA that can be inserted into organism for cloning purpose.…

Q: Why is the technique used to clone mammals called somatic cellnuclear transfer?

A: A somatic cell is a cell that takes part in the composition of the body of an organism and divides…

Q: Describe a series of experiments to SELECT and/or SCREEN for strains containing mutations that…

A: There is a chemical called styrene which is used in many industries to make latex, polystyrene…

Q: Bacteriophage lambda is as one of the routinely used molecular cloning vectors, which alsoserved as…

A: Gene cloning is a process in which our gene of interest is located into the gene and copied (cloned)…

Q: Which is an indicator that all transposable elements have a mechanism for insertion? A) Transposable…

A: Transposable elements or Transposons are the genetic elements which can move from one position to…

Q: Describe the effect of the deletion on co-transformation of pure and pepN genes that are isolated…

A: Transformation is a process of transforming the genes of one strain and then transferring it to…

Q: describe how DNA shuffling and molecular breeding are used to generate hybrid genes and the…

A: DNA shuffling or molecular breeding is an in-vitro molecular method, where mutant genes are created…

Q: Describe the Ti plasmid binary vector system used in plant transformation and provide details of how…

A: Plasmids are small circular double-stranded DNA molecules present in bacterial cells and other…

Q: vector pUST, gene W, and the restriction cut sites. What are 3 cutting strategies for cloning gene W…

A: *Cloning means producing an organisms with identical DNA either by natural or through artificially .…

Q: Describe how you would genetically design a superbugresistant to b-lactams, methicillin,…

A: Superbugs are strains of viruses, bacteria, fungi, and parasites that are resistant to most the…

Q: Outline the strategies commonly used to detect a cloned target gene in E. coli.

A: With the advancement of technologies, various divergent types of recombinant protein are being…

Q: What role did restriction endonucleases play in localizing the gene associated with cystic fibrosis?

A: The gene responsible for cystic fibrosis was isolated from restriction endonuclease digests.

Q: How Hfr strains allow genetic mapping of the E. coli chromosome ?

A: Bacteria are prokaryotic organism which contains a circular double-stranded chromosomal DNA and…

Q: 2. a. Referring to the article "Crystal structure of bacterial succinate:quinone oxidoreductase…

A: Here we are concerned about the bacterial SQR (Succinate Quinone Oxidoreductase) protein. Bacterial…

Q: What is a recombinant microorganism? Discuss examples.

A: Recombination – this is a process used to produce a different combination of alleles by breaking the…

Q: T. Miyake and M. Demerec examined proline-requiring mutations in the bacterium Salmonella…

A: Transduction is the process by which a virus transfers genetic material from one bacterium to…

Q: Identify mutant genes by plasmid library transformation?

A: A plasmid is a small extrachromosomal circular DNA molecule found in bacteria. Transformation is the…

Q: For each of the features present on the plasmid (vector) indicate whether it is needed for use…

A: For each of the features present on the plasmid (vector) it is needed to indicate weather it is used…