before the advent of recombinant DNA technology why was it so difficult for generations to map human genes by using pedigrees how did recombinant DNA technology help move things forward.
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before the advent of recombinant DNA technology why was it so difficult for generations to map human genes by using pedigrees how did recombinant DNA technology help move things forward. |
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- Before the advent of recombinant DNA technology, why was it so diffcult for geneticists to map human genes using pedigrees? How did recombinant DNA technology help move things forward?What impact has recombinant DNA technology had on genetics and society?A researcher used polymerase chain reaction (PCR) to test four individual nematodes for the presence of a mutation in Gene Y. When amplified, the fragments of DNA containing the mutated gene alleles were 250 base pairs long and the normal (wild type) alleles were 350 base pairs long. To identify the genotypes of the individual nematodes, the researcher used gel electrophoresis to visualize the PCR results. 1.000 bp 500 bp 300 bp 200 bp 100 bp Remember that these nematodes are diploid organisms and as such, inherited two copies of Gene Y. Which of the following statements is a correct analysis of these results a) Nematode As homozygous for this gene and has two copies of the mutated allele. O b) Nematode C is heterozygous for this gene and has one copy of the mutated allele and one copy of the normal (wild type) allele. O) Nematode D is heterozygous for this gene and has one copy of the mutated allele and one copy of the normal (wild type) allele. ) d) Nematode B is homozygous for this…
- Why are model organisms so useful in genetics research? Choose one model organism and explain an experiment that taught us about human genetics. Why was that organism a good choice for the experiment?What is DNA fingerprinting? what is its application/s (legally and medically) and limitation/s? How does this technology work? i.e in genomic DNA identification? What is its molecular basis? How does molecular biology paved way for the emergence of this technology?If you were starting with 25 molecules of DNA in a PCR reaction, how much DNA would you expect to have after 25 cycles of amplification? 3,000 molecules 8.2 x 108 molecules 3.3 x 107 molecules 2.5 x 1025 molecules f6 f8 fg f10 fu 15 8 & 6. 8. 5
- Clearly, all humans have variations in their DNA sequences. How is it possible to sequence the human genome if every individual is unique? How was the diversity of the human genome addressed?Is genetic engineering and recombinant DNA technology helpful to society? Why?You are studying a new gene “X” that you think controls skin color in Bearded Dragons. In order to determine what gene X does you need lots of gene X DNA to work with. So, you decide to amplify it through the process known as PCR. You have a forward and a reverse primer that have been designed to gene Xf – a gene in frogs that is similar to the one found in the Bearded Dragon. You are pretty sure that these two primers will work in Bearded Dragons, so you decide to go ahead with the PCR. In frogs, the Forward Xf primer binds gene Xf between nucleotides 32 and 47. The Reverse Xf primer binds between nucleotides 1110 and 1135. What size PCR product would these two primers create if they amplified the frog gene Xf?? bp
- Imagine you've been offered a deal from a genomics company. You can get a free genome sequence – an analysis of all your DNA that includes a report of your ancestry, traits and a medical profile. The medical profile tells you about diseases for which you have a low risk of getting, and also those you have a high risk of getting. Are you interested? Why or why not?If you had the ability to do gene editing with ONE gene for the betterment of human kind, which one would you choose, and why? Assume you could either change an abnormal allele associated with a disease, such as the cystin gene associated with Cystic Fibrosis to its normal wild type, or add a pre-existing human allele to a genome.Humans have engaged in genetic manipulation for millennia, producing plant and animal varieties through selective breeding and hybridization that significantly modify genomes of organisms. Why do you think modern genetic engineering, which often entails introducing or modifying only one or a few genes, has met with so much opposition? Should some forms of genetic engineering be of greater concern than others? Explain.