Bacteria and other microbes can be used to "clean up" an oil spill by breaking down oil into carbon dioxide and water. Twosamples isolated from the Deepwater Horizon leak in the Gulf of Mexico were labeled A and B. The DNA of each was isolatedand the percent thymine measured in each sample. Sample A contains 19.3% thymine and sample B contains 27.9% thymine.Assume the organisms contain normal double-stranded DNA and predict the composition of the other bases.percent adenine in sample A:percent guanine in sample A:percent cytosine in sample A:percent adenine in sample B:%A%G%C% A

Nucleic acids (DNA or RNA) are formed by four types of nucleotides. They are adenine, guanine…

Answered: Bacteria and other microbes can be used…

Biochemistry

9th Edition

ISBN:9781319114671

Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Chapter1: Biochemistry: An Evolving Science

Section: Chapter Questions

Problem 1P

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Similar questions

Assume that there are horizontal gene transfers between two completely different bacterial species. In one case it is a plasmid that is transmitted via conjugation, in the other case it is it is a part of the bacterial chromosome that is transferred via transformation. In which of the two cases is it likely that the transferred DNA will be present? left and can function in the recipient cells? Explain the biological background to your answer
In DNA-DNA Hybridization, sequences from two separate organisms that show partial hybridization suggest _______. a) They are related b) suggest they are not related c)suggest that they are the same organism
DNA is isolated from concentrated ocean water. One genome is studied, and the proportion of each nucleotide is A = 15%, C = 35%, G = 35% and T = 15%. Is this genome from a dsDNA or ssDNA organism? Explain your reasoning. Is this organism AT-rich or GC-rich?
IF A DOUBLE-STRAND DNA SAMPLE WAS COMPOSED OF 10 PERCENT THYMINE, WHAT WOULD BE THE PERCENTAGE OF GUANINE?
How was this wrong? Could someone show me to correction :)
To be able to properly analyze a sample of DNA, you need to have at least 1 µg of the DNA of interest. Assumptions: PCR is run with 30 cycles; Molecular weight per nucleotide in DNA is about 660 g/bp (330 g/bp for single stranded DNA or RNA); bp = base pair. a. Given that you can use the Polymerase Chain Reaction (PCR) to amplify DNA from the original sample and the size of the fragment to be amplified is 10,000 nucleotides long, estimate how many DNA molecules you need to obtain the minimum amount required to analyze the sample. b. Suggest a way to increase your sensitivity, i.e. amplify the DNA starting from even fewer molecules of template DNA.
Bacteria and other microbes can be used to "clean up" an oil spill by breaking down oil into carbon dioxide and water. Two samples isolated from the Deepwater Horizon leak in the Gulf of Mexico were labeled A and B. The DNA of each was isolated and the percent thymine measured in each sample. Sample A contains 18.1% thymine and sample B contains 28.9% thymine. Assume the organisms contain normal double-stranded DNA and predict the composition of the other bases. percent adenine in sample A: SA percent guanine in sumple A: percent cytosine in sample A: percent adenine in sample B}: percent guanine in sample B 5 percent cytosine in sample B Beth samples are then denatured to remove the secondary structure. Which will have the higher temperature to densture!
Bacteria and other microbes can be used to "clean up" an oil spill by breaking down oil into carbon dioxide and water. Two samples isolated from the Deepwater Horizon leak in the Gulf of Mexico were labeled A and B. The DNA of each was isolated and the percent thymine measured in each sample. Sample A contains 19.3% thymine and sample B contains 27.9% thymine. Assume the organisms contain normal double-stranded DNA and predict the composition of the other bases. percent adenine in sample B: percent guanine in sample B: percent cytosine in sample B: Both samples are then denatured to remove the secondary structure. Which will have the higher temperature to denature? A B % A %G %C
After plasmid isolation, you take 0.5 uL of the sample and mix it with 1 uL of TE buffer. The absorbance of this sample at 260 nm is 0.8 and the average extinction coefficient of double-stranded DNA at 260 nm is 0.020 (μg/ml)−1 cm−1. What is the concentration of the undiluted sample? 120 μg/mL 80 μg/mL 40 μg/mL 12 μg/mL
Write a result paragraph of the isolation and purification of bacteriophage with E. coli Top10 with the following results: At 10^-1 to 10^-3 no plaques were formed. At 10^-4 too numerous plaques were formed. At 10^-5, 10^-6 and 10^-7 countable plates of 22, 7 and 1 respectively. At 10^-8 and 10^-9 no plaques formed but present of host bacteria.
Imagine that you are a student in Alfred Hershey and Martha Chase’s lab in the late 1940s. You are given five test tubes containing E. Coli bacteria infected with T2 bacteriophages that have been labeled with either 32P or 35S. Unfortunately, you forget to mark the tubes and are now uncertain about which tubes is which. You performed their blender experiment and got the following results. Which tube out of these 5 contains E. Coli infected with 32P-labeled phage? Explain your answer.
You are given two samples of DNA, one from Clostridium perfringens and the other from Mycobacterium tuberculosis. Unfortunately, the labels on the tubes of DNA were accidentally removed, and you do not know which DNA sample belongs to which organism. As you were doing some work with Escherichia coli in the lab, you also have some of its DNA available as well. The %G + %C values for each of the organisms is known (C. perfringens, 27%; M. tuberculosis, 67%; E. coli, 50%). Based upon this information, how could you quickly determine the source of the DNA for your two samples?

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