At room temperature sucrose is hydrolyzed by the catalytic action of the enzyme sucrase as follows: sucrase sucrose – products Starting with a sucrose concentration C40 = 1.0 millimol/liter and an enzyme concentration Ceo = 0.01 millimol/liter, the following kinetic data are obtained in a batch reactor (concentrations calculated from optical rotation measurements): СА (mmo/L) t (hr) 0.84 0.68 0.53 0.38 0.27 0.16 0.09 0.04 0.018 0.006 0.003 1 3 4 5 7 8 10 11 Determine whether these data can be reasonably fitted by a kinetic equation of the Michaelis- Menten type, or
At room temperature sucrose is hydrolyzed by the catalytic action of the enzyme sucrase as follows: sucrase sucrose – products Starting with a sucrose concentration C40 = 1.0 millimol/liter and an enzyme concentration Ceo = 0.01 millimol/liter, the following kinetic data are obtained in a batch reactor (concentrations calculated from optical rotation measurements): СА (mmo/L) t (hr) 0.84 0.68 0.53 0.38 0.27 0.16 0.09 0.04 0.018 0.006 0.003 1 3 4 5 7 8 10 11 Determine whether these data can be reasonably fitted by a kinetic equation of the Michaelis- Menten type, or
Introduction to Chemical Engineering Thermodynamics
8th Edition
ISBN:9781259696527
Author:J.M. Smith Termodinamica en ingenieria quimica, Hendrick C Van Ness, Michael Abbott, Mark Swihart
Publisher:J.M. Smith Termodinamica en ingenieria quimica, Hendrick C Van Ness, Michael Abbott, Mark Swihart
Chapter1: Introduction
Section: Chapter Questions
Problem 1.1P
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Transcribed Image Text:At room temperature sucrose is hydrolyzed by the catalytic action of the enzyme
sucrase as follows:
sucrase
sucrose
products
Starting with a sucrose concentration C40 = 1.0 millimol/liter and an enzyme concentration Ceo =
0.01 millimol/liter, the following kinetic data are obtained in a batch reactor (concentrations
calculated from optical rotation measurements):
CA (mmol/L)
0.84
0.68
0.53
0.38
0.27
0.16
0.09
0.04
0.018
0.006
0.003
t (hr)
1
2
3
4
5
7
8
9
10
11
Determine whether these data can be reasonably fitted by a kinetic equation of the Michaelis-
Menten type, or
k3C,CeO
Са + См
-rA =
If the fit is reasonable, evaluate the constants k3 and CM.
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