At room temperature sSucrose is ydrolyzed by the catalytic action of the enzyme sucrase as followS: sucrase sucrose products Starting with a sucrose concentration CAO = 1.23 millimol/liter and an enzyme concentration CEO = 0.04 millimol/liter, the following kinetic data are obtained in a batch reactor (concentrations calculated from optical rotation measurements): CA, millimol/liter | 0.84 0.68 0.53 0.38 0.27 0.16 0.09 0.04 0.018 0.006 0.0025 t, hr 1 2 3 4 5 6 7 8 9 10 11 -TA = where CM = Michaelis constant %3D CA + CM If the fit is reasonable, evaluate the constants k3 and CM. Solve by the integral method.

Introduction to Chemical Engineering Thermodynamics
8th Edition
ISBN:9781259696527
Author:J.M. Smith Termodinamica en ingenieria quimica, Hendrick C Van Ness, Michael Abbott, Mark Swihart
Publisher:J.M. Smith Termodinamica en ingenieria quimica, Hendrick C Van Ness, Michael Abbott, Mark Swihart
Chapter1: Introduction
Section: Chapter Questions
Problem 1.1P
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At room temperature sucrose is hydrolyzed by the catalytic action of the enzyme sucrase as follows:
sucrase
sucrose
products
Starting with a sucrose concentration CAO = 1.23 millimol/liter and an enzyme concentration CEO = 0.04 millimol/liter, the following kinetic data are obtained in a
batch reactor (concentra tions calculated from optical rotation measurements):
CA, millimol/liter | 0.84 0.68 0.53 0.38 0.27 0.16 0.09 0.04 0.018 0.006 0.0025
t, hr
1
3
4
5
6
7
8
9
10
11
K3CACEO
-TA =
where
CM = Michaelis constant
Ca + CM
If the fit is reasonable, evaluate the constants k3 and CM. Solve by the integral method.
Transcribed Image Text:At room temperature sucrose is hydrolyzed by the catalytic action of the enzyme sucrase as follows: sucrase sucrose products Starting with a sucrose concentration CAO = 1.23 millimol/liter and an enzyme concentration CEO = 0.04 millimol/liter, the following kinetic data are obtained in a batch reactor (concentra tions calculated from optical rotation measurements): CA, millimol/liter | 0.84 0.68 0.53 0.38 0.27 0.16 0.09 0.04 0.018 0.006 0.0025 t, hr 1 3 4 5 6 7 8 9 10 11 K3CACEO -TA = where CM = Michaelis constant Ca + CM If the fit is reasonable, evaluate the constants k3 and CM. Solve by the integral method.
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