At room temperature sSucrose is ydrolyzed by the catalytic action of the enzyme sucrase as followS: sucrase sucrose products Starting with a sucrose concentration CAO = 1.23 millimol/liter and an enzyme concentration CEO = 0.04 millimol/liter, the following kinetic data are obtained in a batch reactor (concentrations calculated from optical rotation measurements): CA, millimol/liter | 0.84 0.68 0.53 0.38 0.27 0.16 0.09 0.04 0.018 0.006 0.0025 t, hr 1 2 3 4 5 6 7 8 9 10 11 -TA = where CM = Michaelis constant %3D CA + CM If the fit is reasonable, evaluate the constants k3 and CM. Solve by the integral method.
At room temperature sSucrose is ydrolyzed by the catalytic action of the enzyme sucrase as followS: sucrase sucrose products Starting with a sucrose concentration CAO = 1.23 millimol/liter and an enzyme concentration CEO = 0.04 millimol/liter, the following kinetic data are obtained in a batch reactor (concentrations calculated from optical rotation measurements): CA, millimol/liter | 0.84 0.68 0.53 0.38 0.27 0.16 0.09 0.04 0.018 0.006 0.0025 t, hr 1 2 3 4 5 6 7 8 9 10 11 -TA = where CM = Michaelis constant %3D CA + CM If the fit is reasonable, evaluate the constants k3 and CM. Solve by the integral method.
Introduction to Chemical Engineering Thermodynamics
8th Edition
ISBN:9781259696527
Author:J.M. Smith Termodinamica en ingenieria quimica, Hendrick C Van Ness, Michael Abbott, Mark Swihart
Publisher:J.M. Smith Termodinamica en ingenieria quimica, Hendrick C Van Ness, Michael Abbott, Mark Swihart
Chapter1: Introduction
Section: Chapter Questions
Problem 1.1P
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Transcribed Image Text:At room temperature sucrose is hydrolyzed by the catalytic action of the enzyme sucrase as follows:
sucrase
sucrose
products
Starting with a sucrose concentration CAO = 1.23 millimol/liter and an enzyme concentration CEO = 0.04 millimol/liter, the following kinetic data are obtained in a
batch reactor (concentra tions calculated from optical rotation measurements):
CA, millimol/liter | 0.84 0.68 0.53 0.38 0.27 0.16 0.09 0.04 0.018 0.006 0.0025
t, hr
1
3
4
5
6
7
8
9
10
11
K3CACEO
-TA =
where
CM = Michaelis constant
Ca + CM
If the fit is reasonable, evaluate the constants k3 and CM. Solve by the integral method.
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