Can you help what the charge is at ph 9.5? At pH 9.5, the charge on the N-terminal amino group is+1-1 Peptide lonizationConsider a peptide with the sequence Ser-His-Glu-Cys-Arg-Gln.You will be asked to state the charge on the functional groups of thispeptide at pH 1 and pH 9.5.You will also be asked to calculate the net charge on the peptide. You mayfind it convenient to write down the charge on each group on a piece ofpaper as you go along, so that you can add them up at the end.Assume the following generic pK, values for ionizable functional groups:a-carboxyl:side-chain carboxyl:imidazole:3.14.16.0a-amino:8.0thiol:8.3E-amino:10.8aromatic hydroxyl:guanidino:10.912.5

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Answered: At pH 9.5, the charge on the N-terminal…

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Biochemistry

At pH 9.5, the charge on the N-terminal amino group is

Biochemistry

9th Edition

ISBN:9781319114671

Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Chapter1: Biochemistry: An Evolving Science

Section: Chapter Questions

Problem 1P

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Question

Can you help what the charge is at ph 9.5?

At pH 9.5, the charge on the N-terminal amino group is
+1
-1

Peptide lonization
Consider a peptide with the sequence Ser-His-Glu-Cys-Arg-Gln.
You will be asked to state the charge on the functional groups of this
peptide at pH 1 and pH 9.5.
You will also be asked to calculate the net charge on the peptide. You may
find it convenient to write down the charge on each group on a piece of
paper as you go along, so that you can add them up at the end.
Assume the following generic pK, values for ionizable functional groups:
a-carboxyl:
side-chain carboxyl:
imidazole:
3.1
4.1
6.0
a-amino:
8.0
thiol:
8.3
E-amino:
10.8
aromatic hydroxyl:
guanidino:
10.9
12.5

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