Answered: Assuming the use of the same stationary…

Fundamentals Of Analytical Chemistry

9th Edition

ISBN:9781285640686

Author:Skoog

Publisher:Skoog

Chapter29: Mass Spectrometry

Section: Chapter Questions

Problem 29.7QAP

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A particular mixture was seperated into three components usin paper chromatography. the Rf value of dye A is 0.78, the Rf of dye B is 0.43 and the Rf of dye C is 0.19. What component of the mixture A,B,C is most attracted in the stationery phase and why?
When stating the Rf value of a compound, why is it also necessary to state the mobile phase that was used?
What characteristic makes a photodiode array spectrophotometer suitable for measuring the spectrum of a compound as it emerges from a chromatography column and a dispersive spectrophotometer not suitable? What is the disadvantage of the photodiode array spectrophotometer?
As1...If a TLC plate shows only one spot with an Rf value of 0.98, does this mean the sample is pure? Briefly explain why or why not.
The concentration of Na* in the sea water sample was determined with atomic fluorescence spectroscopy (AFS). 35.00 mL of the sea water sample produce an AFS signal of 0.425. Then, 5.00 mL of 0.00215 M Nat standard were added to another 35.00 mL of the sea water sample, and this spiked sample gives an AFS signal of 0.724. What would be the concentration of Na* in the sea water sample? 0.0268 M 0.00753 M 0.0109 M 0.587 M 0.00324 M
What are the advantages of using the KBr in IR spectroscopic sample preparation? Please shortly answer at your own words. Answer should be to the point (specific 4-5 lines).
You and another student were each given an unknown compound. Both samples contained colorless material. You each used the same brand of commercially prepared TLC plate and developed the plates using the same solvent. Each of you obtained a single spot of Rf = 0.75. Were the two samples necessarily the same substance? How could you prove unambiguously that they were ily identical using TLC?
If a molecule fluoresces, what are the advantages of using a fluorimeter instead of a molecular spectrophotometer to detect the fluorophore. How do these instruments differ from one another? How are they the same?
5. Would you expect that changing the solvent will change the Rf value of the specific solute? Why?6. Suggest simple reasons why the Ni2+ and Fe3+ ions have different Rf values?7. What forces causes the eluting solution to move the chromatographic support material?
A particular mixture was seperated into three components usin paper chromatography. the Rf value of dye A is 0.78, the Rf of dye B is 0.43 and the Rf of dye C is 0.19. What component of the mixture A,B,C is most soluble in the mobile phase and why?
Calculate the chemical shift in ppm for a proton that has resonance at 128 hz downfield from TMS on spectophotometer that operates at 60 mhz what would be the value of shift for a spectophotometer which operates at 100 mhz
i) Find the differences between the AES and XPS in terms of principle and information obtained from the spectra. ii) Why AFM is mostly utilized microscopic method for the determination of the thickness of the film?

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Assuming the use of the same stationary and mobile phases, how would the Rf of a compound change ifI had used a TLC plate that was twice as long as the plate used in my experiment?