During your first day on the job as a research assistant, you are preparing 50 mL of hybridization solution with the following components and working concentrations: 50% formamide 5X sodium salt citrate 0.5% sodium dodecyl sulfate You start from the following concentrated stock solutions: 100% formamide 20X sodium salt citrate 20% sodium dodecyl sulfate How much of each concentrated stock solution and water will you add?

Part III: Determination of Saturated Solutions Molarity (saturated) Tube # Temperature (°C) Molarity (dilute) Absorbance 2 0.6 0.157 20.1 0.291 4 37.6 0.351 50.1 0.500 6. 70.9 0.714 show calculation for Tube #2, Molarity (dilute) (use graph equation): show calculation for Tube #2, Molarity (saturated): 1. Is the relationship between solubility and temperature direct or inverse? Explain.

3. Standard white vinegar you can buy in the grocery store is 5% concentration. That means 5% of the liquid vinegar is acetic acid and 95% of the solution is water. In a hardware store, you can buy industrial strength vinegar, which is 30% concentration. This means that 30%% of the vinegar is acetic acid, and the remaining 70% is water. Samuel does another experiment, this time with 5% vinegar and 30% vinegar. He sets up two science fair volcanoes (in no particular order), each with the same temperature, mass of baking soda and volume of vinegar. But one volcano uses 5% vinegar and the other volcano uses 30% vinegar. He measures the volume of gas production for the first minute of each reaction, and he records the data below. Volcano # 1 Volume of gas produced (ml.) vs. Time (s) for Volcano #1 Volume of gas produced (mL) Time (s) 60 E 50 10 25 40 20 38 30 40 46 50 30 20 • Volume of gas produced (ml.) 50 52 53 10 60 20 40 60 80 Time (s) Volcano # 2 Volume of gas Volume of gas produced…

The mixture contains benzoic acid, 2-napthol, and naphthalene. Dissolve the mixture in the polar solvent ether. When you react the mixture with 10% NaHCO3(sodium bicarbonatea weak base in aqueous solution) reacts with benzoic acid and forms sodium benzoate an ionic compound (similar to NaCl) that dissolves in aqueous solution (heavier than ether in density) and goes to the bottom of the centrifuge tube or to the bottom of the separatory funnel forming two layers. Then remove the bottom aqueous layer and collect in a beaker labeled as bicarbonate extract. Acidify the bicarbonate extract in the beaker to give benzoic acid. The top ethereal layer contains in the centrifuge tube or separatory funnel 2-napthol and naphthalene. To this top layer add 10% NaOH (sodium hydroxideis a strong base in aqueous solution reacts with only 2-napthol, a weak acid forming 2-napthoxide an ionic compound soluble in aqueous solution (similar to NaCl) that goes to the bottom of the centrifuge tube or to…

Start with 15mL of 0.100M HCl. Pipette 10mL of the 0.100M HCl into a 250mL beaker. Then dilute it to around 100mL with distilled water. What would the concentration of HCl be?

complete the flowchart

Chemistry Because the three possible unknowns (cinnamic acid, vanillin and 4- hydroxybenzaldehyde) have either carboxylic acid or phenol functional groups, they are all weak acids. • How will this weak acidity affect the freezing point depression? [Select] • What is the source of this affect? [Select] • How will the calculated molecular mass differ from the actual value? [Select] Because the three possible unknowns (cinnamic acid, vanillin and 4- hydroxybenzaldehyde) have either carboxylic acid or phenol functional groups, they are all weak acids. • How will this weak acidity affect the freezing point depression? [Select] [Select] The solution freezing point is lower than it should be. The solvent freezing point is higher than it should be. The solvent freezing point is lower than it should be. The solution freezing point is higher than it should be. value? [Select] n the actual

TRUE OR FALSE 1. Medicated elixirs are advantageous in adjusting the dose that may be increased or decreased by simply taking more or less of the elixir.  2. It is impossible to increase or decrease the amount taken of one ingredient without and automatic and corresponding adjustment in the dose taken of the other.  3. Then the alcoholic solution is added to the aqueous solution in order to maintain the highest possible alcoholic strength. 4. Saccharin is used for elixirs with low alcoholic content required only in small amounts. 5. Proportion of alcohol varies widely in elixirs since the individual components. 6. The final mixture of elixir will be clear since the separation of some of the flavoring oils by the reduced alcoholic concentration.  7. Permission of the oil globules oil to coalesce leads to difficulty in filtration. 8. Wetting the filter paper and collection flask using the solvent ensures continued solubility of the alcoholic ingredients.  9. Elixirs are best stored in…

You are given an 18% solution of sodium imidazole. What mass of imidazole (in g) would be in 20 mL of this solution?    a. You need to use the sodium imidazole stock solution from Q11 to make a dilute imidazole solution (final volume 180 mL) at      a final concentration of 0.2 M. The molecular weight of imidazole is 90 g/mole.    What volume of imidazole stock would you use (mL)?     b. You need to use the sodium imidazole stock solution from Q11 to make a dilute imidazole solution (final volume 180 mL) at a final concentration of 0.2 M. The molecular weight of imidazole is 90 g/mole.    What volume of water would you add (mL)?

A pharmacist has 50 mL of a 0.5% Gentian Violet solution, what will be the final ratio strength if he dilutes this solution to 1250 mL with purified water? 1:5000 1:500 1:1000 1:8