If a molecule fluoresces, what are the advantages of using a fluorimeter instead of a molecular spectrophotometer to detect the fluorophore. How do these instruments differ from one another? How are they the same?
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If a molecule fluoresces, what are the advantages of using a fluorimeter instead of a molecular spectrophotometer to detect the fluorophore. How do these instruments differ from one another? How are they the same?
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- What types of samples can be analyzed by IR?How are substances that are opaque (nottransparent) handled using this technique?Many ions form colored solutions when dissolved in water. For example, aqueous Cu2+ solutions are light blue and aqueous Ni2+ solutions look light green. These metals in particularare often found in stainless steel. Would you expect their presence in your samples to interfere in the spectrophotometric analysis of permanganate at 525 nm? Why or why not? I think the answer is that I would expect the Cu2+ in my samples to interfere but not Ni2+ because the Ni2+ is green, signifying a higher wave length that the spectrometer wouldn't be able to pick up on because it was only set to 525 (blue-ish) where as the green is 530-ish. I just want to make sure thanks!What is the significance of the max of a spectrum? What are the other uses of the spectrophotometer?
- You measure a sample in your spectrophotometer that has an absorbance of 1.00. What is the concentration of your sample in M.0.1040 g of a solid sample containing copper was dissolved by acid and transferred to a 250.00 mL volumetric flask and volume was made up with distilled water. The solution was then diluted by a factor of 20. The final solution was analyzed by atomic absorption spectrophotometry and the concentration was found be 3.109 ppm, What is the weight percentage of copper in the original solid sample? Keep four significant figures in your final answer. Do not try to include the "%" in your answer. e.g. if your final answer is 10.01%, only write 10.01 in the box.Explain why the concentration of zinc cannot be determined directly using a spectrophotometer.
- Quantitative Analysis by External Standard Method Calcium in a juice sample is determined by atomic absorption spectrophotometry. A stock solution of calcium is prepared by dissolving 1.834 g of CaCl, 2H20 in water and diluting to 1000.0 mL. A substock calcium solution was prepared by transferring 10.00 mL of the stock to a 100.00 mL volumetric flask and diluting to the mark. Three standard solutions of calcium were prepared by transferring 2.500 mL. 5.00 mL and 10.00 mL of substock to 50.00 mL volumetric flasks and diluting to the mark. The sample is prepared by transferring 1.000 mL of fruit juice from the original bottle to a 25.00 mL flask and diluting to the mark. Strontium chloride is added to all solutions before dilution to avoid phosphate interference. Instrumental response of the standards is measured against the blank, and the following readings are recorded: 9.10, 18.6 and 37.0. The sampie reading is 28.1. Using the multipoint method, find the concentration of calcium in…0.1120 g of a solid sample containing copper was dissolved by acid and transferred to a 250.00 mL volumetric flask and volume was made up with distilled water. The solution was then diluted by a factor of 25. The final solution was analyzed by atomic absorption spectrophotometry and the concentration was found be 2.982 ppm, What is the weight percentage of copper in the original solid sample? Keep four significant figures in your final answer.why is water used as a blank for Spectrophotometry?
- If the standard addition intercept is the major source of uncertainty, find the uncertainty in the concentration of Sr in tooth enamel in parts per million. Find the 95% confidence interval for Sr in tooth enamel.Why do we need to prepare a standard curve before we can use Beer's Law to determine the concentration of a solution? O We don't have to create a standard curve so long as we choose the appropriate wavelength. Because the standard curve tells us the absorbance of our sample Because the standard curve is used to determine the extinction coefficient Because the standard curve indicates what wavelength to set the spectrophotometer toWhat does a refractometer reading of 2 mean when distilling ethanol?
