Assume you are trying to develop a GC-MS instrument which would be used in the field (unit must be portable and robust) for the identification of relatively small compounds (<500 g/mol). Assuming the GC (gas chromatograph) will separate the components prior to the MS, what type of ionization method (i.e. EI, CI, ESI, MALDI) and detector (i.e. magnetic sector, magnetic double sector, quadrupole, ion trap, or time-of flight) would you choose and why?

Assume you are trying to develop a GC-MS instrument which would be used in the field (unit must be portable and robust) for the identification of relatively small compounds (<500 g/mol). Assuming the GC (gas chromatograph) will separate the components prior to the MS, what type of ionization method (i.e. EI, CI, ESI, MALDI) and detector (i.e. magnetic sector, magnetic double sector, quadrupole, ion trap, or time-of flight) would you choose and why?

Fundamentals Of Analytical Chemistry

9th Edition

ISBN:9781285640686

Author:Skoog

Publisher:Skoog

Chapter33: High-performance Liquid Chromatography

Section: Chapter Questions

Problem 33.12QAP

See similar textbooks

Similar questions

Tim is interested in analyzing for Pyraclostrobin (pesticide) in strawberries using QuECHers method for extraction and clean up before analysis with HPLC-uv. The concentration of the Pyraclostrobin in the fruits that he sampled is thought to be between 1-10 ng pesticide per g fruit fresh weight. The HPLC-uv instrument he is using has a LoQ of 5 ppb for Pyraclostrobin. Tim is starting out with 500 mg of the sliced fruit
a) In any gas chromatographic analysis, exceeding a column's maximum temperature limit will usually result in column bleeding. i) Define what is column bleeding. Give another approach other than reducing column final temperature to prevent ii) column bleeding. b) Flame ionization detector (FID), electron capture detector (ECD) and mass spectrometry detector (MSD) are widely used with gas chromatographic separations. i) Explain why FID is sensitive to organic compounds but insensitive to noncombustible gases such as CO2, SO2, CO and NO. ii) State the type of compounds that responds to ECD and MSD.
Analysis of an unknown plant extract yield two peaks X and Y in a chromatogram, 15.65 and 17.03 min respectively in a 30.0 cm column. The peak base width for X is 1.06 and 1.15 for Y. The average number of plates in the column is
A gas chromatogram of a mixture of toluene and ethyl (a) Measure w1/2 for each peak to the nearest 0.1 mm. When the thickness of the pen trace is significant relative to the length being measured, it is important to take the pen width into account. It is best to measure from the edge of one trace to the corresponding edge of the other trace, as shown at the bottom of the left column. (b) Find the number of theoretical plates and the plate height for each peak.
What are the mechanisms of samples separation work in Thin layer Chromatography? Please shortly answer at your own words. Answer should be to the point (specific 4-5 lines).
1
4.Consider the peaks for pentafluorobenzene and benzene in the gas chromatogram shown here. The elution time for unretained solute is 1.06 min. The open tubular column is 30.0 m in length and 0.530 mm in diameter, with a layer of stationary phase 3.0 μm thick on the inner wall. a) Measruing the width, w, at the baseline on the chromatogram, find the number of plates for these two compounds b) Use your answer to (a) to find the resolution between the two peaks c) Using the number of plates N=sqrtN1*N2 with the values from (a) calcuate what the resolution should be and compare your answer with the measured resolution in b
A young researcher set out to develop a method for quantifying the polycyclic aromatic hydrocarbon (PAH) content in a contaminated soil sample using open tubular gas chromatography. During the initial stages of method development, the researcher was unable to separate the early eluting compounds, anthracene and naphthalene, with baseline resolution. Select the possible modifications the researcher could make to improve the resolution. use a column with a smaller inner diameter decrease the column length increase the stationary phase thickness increase the temperature choose a different stationary phase
3 questions...
1) Study the chromatograph (below) of a mixture of Compounds A and B, run on the GCs in the teaching labs at CU Boulder. Compound A has the shorter retention time. STAAT 61 1.11 227 RT TYPE AREA XXXX XXXX XXXX AREAS 0.009 55874 44.117 ARIHT 0.61 XX XX XX 1.11 2.27 XX XX XX TOTAL AREA=XX MUL FACTOR=XX 1. What is the retention time of compound A? Compound B? 2. Which compound is present in a larger amount? 3. Which compound has the lower boiling point? 4. What would happen to the retention times of compounds A and B if the column temperature were raised? 5. You suspect that compound B is octane. What can you do to provide supporting evidence for this hypothesis?
How can Gas Chromatography be used to determine identity? Include the theory of how molecules interact with and elute from molecular stationary phase of GC GC column (Hint: BP anf Polarity), the information gained from order of elution, and type of column used. Reaction: Isoamyl alcohol + acetic acid === isoamyl acetate (product
The figure below shows a portion of a GC chromatogram for a mixture of two aromatic compounds labeled A and B. The separation employed a 2.50 meter packed column under isothermal conditions (90 °C) and a flow rate of 10 mL/min. (a) Detector Signal 300.0 250.0 200.0 150.0 100.0 50.0 0.0 1.0 1.1 Unretained 1.2 1.5 time (min) Find the retention times for A and B. 1.7 O