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Answer Questions about your results: 1. Which pH provided the highest pepsin activity? Is that what you expected, based on location of pepsin secreting chief cells in the gastric glands? 2. Would pepsin be active in the mouth? Explain your response: 3. What are the products of protein digestion (subunits of proteins)? inning?)

Answer Questions about your results: 1. Which pH provided the highest pepsin activity? Is that what you expected, based on location of pepsin secreting chief cells in the gastric glands? 2. Would pepsin be active in the mouth? Explain your response: 3. What are the products of protein digestion (subunits of proteins)? inning?)

Biochemistry
Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
Problem 1P
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Chart 3: Pepsin digestion of Protein Tube No. Additives 1. Pepsin 2 3. Pepsin BAPNA 4. water 5. Pepsin BAPNA 9. Pepsin Pepsin BAPNA water BAPNA pH 2.0 Buffer BAPNA pH 2.0 Buffer Boil first, Incubate 37°C pH 2.0 Buffer 37°C pH 2.0 Buffer pH 7.0 Buffer pH 9.0 buffer Incubation 37°C 37°C 37°C 37°C Condition (all 60 min.) Predict results (before lab) Optical density Answer Questions about your results: 1. Which pH provided the highest pepsin activity? Is that what you expected, based on location of pepsin secreting chief cells in the gastric glands? 2. Would pepsin be active in the mouth? Explain your response: 3. What are the products of protein digestion (subunits of proteins)? 4. Why is tube 4 described as "control" for this experiment on effect of pepsin? (hint -what is missing?) 5. Tubes 1 and 2 contained the same substances. Explain why their optical density measurements were different: 6. If you had not run the tube 2 and tube 3 samples, what argument could be made against the statement "Pepsin digests BAPNA"? 7. How did your predictions compare with actual results? Explain: Additional Activity: Find your BMI: - using your height & weight- chart can be found at Mayo Clinic site: https://diet.mayoclinic.org/diet/eat/what-is-your-bmi?xid=nl_MayoClinicDiet_20160426 **At home or at end of lab: Do review Q's PhysioEx#8 (Activity 1: 1-5, Activity 3: 1-3,)** 9.
Transcribed Image Text:Chart 3: Pepsin digestion of Protein Tube No. Additives 1. Pepsin 2 3. Pepsin BAPNA 4. water 5. Pepsin BAPNA 9. Pepsin Pepsin BAPNA water BAPNA pH 2.0 Buffer BAPNA pH 2.0 Buffer Boil first, Incubate 37°C pH 2.0 Buffer 37°C pH 2.0 Buffer pH 7.0 Buffer pH 9.0 buffer Incubation 37°C 37°C 37°C 37°C Condition (all 60 min.) Predict results (before lab) Optical density Answer Questions about your results: 1. Which pH provided the highest pepsin activity? Is that what you expected, based on location of pepsin secreting chief cells in the gastric glands? 2. Would pepsin be active in the mouth? Explain your response: 3. What are the products of protein digestion (subunits of proteins)? 4. Why is tube 4 described as "control" for this experiment on effect of pepsin? (hint -what is missing?) 5. Tubes 1 and 2 contained the same substances. Explain why their optical density measurements were different: 6. If you had not run the tube 2 and tube 3 samples, what argument could be made against the statement "Pepsin digests BAPNA"? 7. How did your predictions compare with actual results? Explain: Additional Activity: Find your BMI: - using your height & weight- chart can be found at Mayo Clinic site: https://diet.mayoclinic.org/diet/eat/what-is-your-bmi?xid=nl_MayoClinicDiet_20160426 **At home or at end of lab: Do review Q's PhysioEx#8 (Activity 1: 1-5, Activity 3: 1-3,)** 9.
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