Animal cell cultures usually require 5% carbon dioxide in the incubator. This gas along with sodium bicarbonate found in the culture medium helps to regulate what property of the cell medium? (i.e. what environmental condition does carbon dioxide/sodium bicarbonate regulate?)

Q: Initially, the solution in side A, with respect to that in side B, is what?   After the system…

A: Introduction Water potential is the measure of the tendency of water to move from one to another…

Q: While measuring the turbidity of a bacterial culture in class, Kevin recorded both the absorbance…

A: Turbidity is the measurement of the suspended particles in a fluid that makes it opaque. Quality…

Q: What is the concentration of cells in the original cell suspension?

A: we can use the dilution formula:C1 V1 = C2 V2where:EXPLANATIONC1 is the initial concentration of the…

Q: the cell potential table found in the Reference Material,  why do you get holes in your aluminum…

A: The natural process that results in the conversion of a reactive metal into a stable metal by…

Q: What salt did Meselson and Stahl use for their ultracentrifuge gradients? O NaCI O NH4CI O CSCI O…

A: Given: DNA replicates semiconservatively. Matthew messelson and Franklin stahl performed an…

Q: )Suppose you were culturing a population of bacteria at 37 degrees C and then reduce the temperature…

A: The maintenance of the fluidity of the plasma membrane is important for cell survival. The membrane…

Q: A dialysis tube that contains 10 mL of a 5% sucrose solution and that is permeable to water but not…

A: Tonicity is the capability of a solution to change the volume of cells by the movement of water.…

Q: (b) Define concentration polarization and polarization modulus. How is polarization modulus…

A: CP is a process of accumulation of retained solutes in the membrane boundary at the feed side. It is…

Q: Based on the following data, which bacteria is most likely a human pathogen? Bacteria E.coli S.…

A: The quantity of light absorbed by the solution or sample is called absorbance or optical density. In…

Q: Complete the following questions for your gram negative rod unknown organism (a) What test did you…

A: The gram-negative rod group is a diverse and extensive category of bacteria characterized by their…

Q: A continuious, stirred-tank bioreactor with an actively growing culture of bacteria is operating at…

A: A bioreactor is a manufactured device or a system which supports biologically active environment…

Q: When combining 1ml of lysosome solution with a concentration of 5mg/ml and 1ml of water what is the…

A: Tiny, simple sack-like structures present in cytoplasm of cells are lysosomes. These are also known…

Q: the 10 mg/mL sample of lysozyme shows a lower activity than 2mg/mL lysozyme sample (refer to the…

A: The most likely explanation for the lower activity of the 10 mg/mL lysozyme sample compared to the 2…

Q: A microorganism was streaked onto 4 nutrient agar plates and the plates were incubated at different…

A: Nutrient agar  A type of general media that supports growth of wide variety of microorganisms.

Q: Identify the movement of water in surrounding medium and intracellular environment, given these…

A: Tonicity is a measurement of the effective osmotic pressure gradient, which is the difference in…

Q: Suppose you have a suspension of HeLa cells with a cell count of 85,000 cellsper ml, and you want to…

A: HeLa cells were discovered in 1950s and is named after the women from whom these cells are taken…

Q: You obtain the following results for the transport of an amino acid into bacteria. Based on this…

A: The cell membrane presents a hydrophobic barrier that separate the interior of the cell from the…

Q: average membrane pore radius for this membrane using this information.

A: Given, Average radii=1nm Conc. of filtrate = 2.17 g/L Conc. of feed-side surface = 8.52 g/L Mass…

Q: Growth of an E. coli bacteria in batch culture is observed. The bacteria are fed with 0.5 g / L…

A: Answer: E.coli = These are the gram negative bacteria can be found in normal human gut flora.

Q: Which of the following is true regarding microbial tests that lead to color change in tubes used to…

A: Option 1 and 4 are correct. There are multiple tests involving color change for bacteria…

Q: What would happen to chemiosmosis if the pH in the inner membrane space was decreased (lower)?

A: Chemiosmosis is the movement of ions across a semipermeable membrane, down their electrochemical…

Q: Please give clinical situations in which you would use isotonic, hypotonic, and hypertonic…

A: Intravenous fluids are used to restore the fluid status in the body also, to facilitate fluid…

Q: . Define diffusion. Is diffusion a passive or an active process? Explain how solute moves in this…

A: There are two types of processes - active and passive that governs the movement of the particles. In…

Q: In the context of flow cytometry data: The Lactobacillus cells also show a clear increase in PerCP…

A: As stained with SYBRGreen (SG), Lactobacillus cells exhibit a noticeable increase in PerCP (red)…

Q: If the bacteria within your medium suddenly die, can an alteration in osmotic balance be the reason…

A: The passage of water through a membrane is known as osmosis. The movement of water to the side of…

Q: determine if osmosis or simple diffusion

A: The main criteria that will be used in this particular question will be the movement of molecules…

Q: The energy required for diffusion of a solute across a cell membrane is: the kinetic energy of the…

A: As according to our guidelines, we are allowed to do a single question at a time, if you want second…

Q: In a reproduction of an experiment famously performed by Louis Pasteur, you cultured yeast in the…

A: Mitochondria are the cell organelles responsible for the production of energy in the cell. They take…

Q: Five dialysis bags, impermeable to sucrose, were filled with various concentrations of sucrose and…

A: Five dialysis bags with varying sugar concentrations are provided. However, the bag's membrane is…

Q: Depicted is a rod-shaped bacterium that can be approximated as a cylinder with two spherical caps, r…

A: The bacteria is a prokaryotic organism and is divided into Gram-negative and Gram-positive bacteria.…

Q: Study the surface protein structure of the influenza virus. Study the localization of the rabies…

A: Microscopy is a fundamental tool in the field of biological research, enabling the visualization and…

Q: Inside a beaker, dialysis membrane A is filled with a 50% glucose solution and dialysis membrane B…

A: Osmosis is the net movement of water from the area of lower solute concentration to an area of a…

Q: Which of the following sample(s) is NOT expected to cause a SUBSTANTIAL decrease in absorbance over…

A: To investigate the characteristics of particles in a solution, scientists sometimes conduct light…

Q: You set up the following two tubes: Tube 1: 1mL of alkaline phosphatase + 4mL of 400 µM p-NPP Tube…

A: ALP is a nonspecific enzyme which hydrolyzes aliphatic, aromatic or heterocyclic compounds. The pH…

Q: What is meant by the statement “orthogonal analysis methods should be used for characterization of…

A: Analytical techniques refer to the methods and tools used in scientific and laboratory settings to…

Q: % change in weight Effect 30 20 10 -10 -20 -30

A: All the organisms need to survive in an environment that is amicable for its growth and development.…

Q: After filtering off the yeast in a difficult process, using a Celite pad to the filtrate which…

A: Celite is a substance that is most commonly used for the purpose of filtration. Celite is composed…

Q: What techniques could we employ to separate 4-pyridoxic acid and quinine in the urine sample?

A: Vitamin B6 is a water-soluble vitamin needed for many metabolic activities because it functions as…

Q: Assuming you had a bacterial culture with a concentratoion of 2x10^9 bacteria/mL, whaat volume would…

A: A microbiological culture, also known as a microbial culture, is a technique for growing…