An analyst was measuring the performance of a HPLC column. The test solute was Toluene (5 µL). Thecolumn was a 4.6 mm i.d. column, 150 mm in length and packed with 5 µm C18 particles. The mobilephase was 40/60 water/methanol and the flow rate was 0.80 mL/min. The void time (to) was 1.86minutesThe retention time of the toluene sample was 14.50 min.The standard deviation of the peak was 7.0542 (s).2a. The mobile phase velocity was?0.0806 mm/s0.1344 mm/s1.344 mm/s1.344 x 10³ mm/s(i)(ii)(iv)2b. The number of theoretical plates was?(i)(ii)(iii)(iv)2c. The height equivalent to a theoretical plate was?1.041x10³ m1.015x105 m9.862x10-6 m1.186x10¹² m(i)(ii)(iii)(iv)4802152102470598482d. The reduced plate height was?(i)2.03(ii)1.97(iii)7.55(iv)2.082e. The column was?(i)(ii)Extremely well packedWell packedPoorly packed(iii)(iv) Insufficient information to decide about the quality of the column

To find out the mobile phase velocity, number of theoretical plates and height equivalent to…

An analyst was measuring the performance of a HPLC column. The test solute was Toluene (5 µL). The column was a 4.6 mm i.d. column, 150 mm in length and packed with 5 µm C18 particles. The mobile phase was 40/60 water/methanol and the flow rate was 0.80 mL/min. The void time (to) was 1.86 minutes The retention time of the toluene sample was 14.50 min. The standard deviation of the peak was 7.0542 (s). 2a. The mobile phase velocity was?

An analyst was measuring the performance of a HPLC column. The test solute was Toluene (5 µL). The column was a 4.6 mm i.d. column, 150 mm in length and packed with 5 µm C18 particles. The mobile phase was 40/60 water/methanol and the flow rate was 0.80 mL/min. The void time (to) was 1.86 minutes The retention time of the toluene sample was 14.50 min. The standard deviation of the peak was 7.0542 (s). 2a. The mobile phase velocity was?

Chemistry

10th Edition

ISBN:9781305957404

Author:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste

Publisher:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste

Chapter1: Chemical Foundations

Section: Chapter Questions

Problem 1RQ: Define and explain the differences between the following terms. a. law and theory b. theory and...

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Question

An analyst was measuring the performance of a HPLC column. The test solute was Toluene (5 µL). The
column was a 4.6 mm i.d. column, 150 mm in length and packed with 5 µm C18 particles. The mobile
phase was 40/60 water/methanol and the flow rate was 0.80 mL/min. The void time (to) was 1.86
minutes
The retention time of the toluene sample was 14.50 min.
The standard deviation of the peak was 7.0542 (s).
2a. The mobile phase velocity was?
0.0806 mm/s
0.1344 mm/s
1.344 mm/s
1.344 x 10³ mm/s
(i)
(ii)
(iv)
2b. The number of theoretical plates was?
(i)
(ii)
(iii)
(iv)
2c. The height equivalent to a theoretical plate was?
1.041x10³ m
1.015x105 m
9.862x10-6 m
1.186x10¹² m
(i)
(ii)
(iii)
(iv)
4802
15210
24705
9848
2d. The reduced plate height was?
(i)
2.03
(ii)
1.97
(iii)
7.55
(iv)
2.08
2e. The column was?
(i)
(ii)
Extremely well packed
Well packed
Poorly packed
(iii)
(iv) Insufficient information to decide about the quality of the column

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