An amino acid on the surface of an enzyme was labelled covalently with 1.5-I AEDANS and it is known that the active site contains a tryptophan residue. The fluorescence quantum yield of tryptophan decreased by 15 per cent due to quenching by 1.5-I AEDANS. What is the distance between the active site and the surface of the enzyme?

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An amino acid on the surface of an enzyme was labelled covalently with 1.5-I AEDANS and it is known that the active site contains a tryptophan residue. The fluorescence quantum yield of tryptophan decreased by 15 per cent due to quenching by 1.5-I AEDANS. What is the distance between the active site and the surface of the enzyme?

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