An amino acid of interest was produced in a fed-batch culture using glucose in the feed medium. The initial volume of the quasi-steady state culture was V0= 500 L, and the nutrient solution containing glucose was added at a constant flow rate of F = 50 L/h.Data: X0 (at the beginning of feeding) = 20 g/L, S0 = 300 g/L, μmax = 0.2 h-1, KS = 0.5 g/L and Y x/s= 0.3 g/ga) determine the concentration and total mass of cells at t = 10 hb) If the product is associated with growth with α = 1.5 and P0 = 0.1 g/L, determine the product concentration at t = 10h.

Given thatVo =500 lF = 50 lhXo =20 glSo =300 glks =0.5 glx/s =0.3 ggumax =0.2 h-1

Answered: An amino acid of interest was produced…

Introduction to Chemical Engineering Thermodynamics

8th Edition

ISBN:9781259696527

Author:J.M. Smith Termodinamica en ingenieria quimica, Hendrick C Van Ness, Michael Abbott, Mark Swihart

Publisher:J.M. Smith Termodinamica en ingenieria quimica, Hendrick C Van Ness, Michael Abbott, Mark Swihart

Chapter1: Introduction

Section: Chapter Questions

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An amino acid of interest was produced in a fed-batch culture using glucose in the feed medium. The initial volume of the quasi-steady state culture was V₀= 500 L, and the nutrient solution containing glucose was added at a constant flow rate of F = 50 L/h.
Data: X₀ (at the beginning of feeding) = 20 g/L, S0 = 300 g/L, μmax = 0.2 h^-1, K_S = 0.5 g/L and Y x/s= 0.3 g/g

a) determine the concentration and total mass of cells at t = 10 h
b) If the product is associated with growth with α = 1.5 and P0 = 0.1 g/L, determine the product concentration at t = 10h.

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