Alfred was tasked to synthesize at least 250.0 ppm of his novel gold nanoparticles (AuNP’s) to be able to perform experiments about the efficacy of AuNP for viral detection. He decided to check whether he still needed to synthesize more or not using a UV Vis spectrophotometer. Fortunately, he already prepared a 100.0 ppm AuNP standard solution beforehand. He got a different aliquot of the standard, added stabilizing solutions, and diluted them to 10.0 mL for the determination of absorbance. Data he obtained is shown below. Table 1. Calibration curve data for AuNP v of 100.0 ppm AuNP standard, mL Absorbance 0.00 0.200 0.50 0.450 1.00 0.510 1.50 0.600 2.00 0.680 3.00 0.810 4.00 0.950 After obtaining the calibration curve, he got a 3.00-mL aliquot of the pre-prepared solution, added stabilizing solution, and diluted it to 10 mL. He got 10mL of the final solution and determined its absorbance to be 0.650. The absorbance of the blank solution for the sample solution was 0.200. a. Fill up the table and determine the calibration curve (Abs vs ppm) with r2 value. Express the equation of the line and r2 in 3 significant figures v of 100.0 ppm AuNP standard, mL [AuNP], ppm True Absorbance 0.00 Answer Answer 0.50 Answer Answer 1.00 Answer Answer 1.50 Answer Answer 2.00 Answer Answer 3.00 Answer Answer 4.00 Answer Answer y = _ x + r2 =

Chemistry

10th Edition

ISBN:9781305957404

Author:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste

Publisher:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste

Chapter1: Chemical Foundations

Section: Chapter Questions

Problem 1RQ: Define and explain the differences between the following terms. a. law and theory b. theory and...

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Alfred was tasked to synthesize at least 250.0 ppm of his novel gold nanoparticles (AuNP’s) to be able to perform experiments about the efficacy of AuNP for viral detection. He decided to check whether he still needed to synthesize more or not using a UV Vis spectrophotometer.

Fortunately, he already prepared a 100.0 ppm AuNP standard solution beforehand. He got a different aliquot of the standard, added stabilizing solutions, and diluted them to 10.0 mL for the determination of absorbance. Data he obtained is shown below.

Table 1. Calibration curve data for AuNP

v of 100.0 ppm AuNP standard, mL	Absorbance
0.00	0.200
0.50	0.450
1.00	0.510
1.50	0.600
2.00	0.680
3.00	0.810
4.00	0.950

After obtaining the calibration curve, he got a 3.00-mL aliquot of the pre-prepared solution, added stabilizing solution, and diluted it to 10 mL. He got 10mL of the final solution and determined its absorbance to be 0.650. The absorbance of the blank solution for the sample solution was 0.200.

a. Fill up the table and determine the calibration curve (Abs vs ppm) with r2 value.

Express the equation of the line and r2 in 3 significant figures

v of 100.0 ppm AuNP standard, mL	[AuNP], ppm	True Absorbance
0.00	Answer	Answer
0.50	Answer	Answer
1.00	Answer	Answer
1.50	Answer	Answer
2.00	Answer	Answer
3.00	Answer	Answer
4.00	Answer	Answer

y = ___ x + ____ r2 = __

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