The calcium ion binds to a certain protein to form a 1:1 complex. The following data were obtained in an experiment. Total Ca2+/uM 60.9 122 186 250. 536 Ca2+ bound to Protein/µM 31.4 51.4 63.6 71.0 83.6 Determine graphically the dissociation constant of the Ca2+-protein complex. The protein concentration was kept at 97 µM for each run. (1 µM = 1×10-6 M.) Ka = 110
The calcium ion binds to a certain protein to form a 1:1 complex. The following data were obtained in an experiment. Total Ca2+/uM 60.9 122 186 250. 536 Ca2+ bound to Protein/µM 31.4 51.4 63.6 71.0 83.6 Determine graphically the dissociation constant of the Ca2+-protein complex. The protein concentration was kept at 97 µM for each run. (1 µM = 1×10-6 M.) Ka = 110
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
Transcribed Image Text:The calcium ion binds to a certain protein to form a 1:1 complex. The following data were obtained in an experiment.
Total Ca2+/µM
60.9
122
186
250.
536
Ca2+ bound to Protein/µM
31.4
51.4
63.6
71.0
83.6
Determine graphically the dissociation constant of the Ca2+-protein complex. The protein concentration was kept at 97 µM for each run. (1 µM = 1×10-6 M.)
Kd
= 110
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