After 20 minutes of incubation at 32°C, you measure the bubble height of CO2 gas collected in a respirometer containing yeast as 62.0 mm. What is the volume of CO2 generated and the fermentation rate? Question options: 99.2 mL; 4.96 mL / min 992 mL; 49.6 mL / min 9.92 mL; .496 mL / min .992 mL; 0.0496 mL / min
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- When the yeast cells have completely re-hydrated, measure out 1.8 mL of well-mixed yeast suspension (0.2% yeast) into each of two new 15 mL centrifuge tubes. Add 200 μL of a 10% (w/v) Sodium Azide*** in YGM solution to one of the 15 mL tubes with yeast suspension. Add 200 μl of YGM to the other 15 mL tube of yeast suspension for your Control. Let both tubes incubate at room temperature for 30 min, vortexing every ~5 minutes What is the % concentration of azide in this sample during metabolic inhibition?What is the % concentration of yeast in this sample during metabolic inhibition?A 10-L stirred tank is used to cultivate mouse myeloma cells. Dry air is sparged from the bottom at 1.0 L/min to supply oxygen to the system. The gas composition at the outler is 16% oxygen, 3.5% CO2, 6.5% water vapor, and balanced nitrogen. The ambient pressure and temperature are 1 atm and 37 °C, respectively. The dissolved oxygen in the reactor is controlled at 20% of saturation with ambient air. a. Assuming the reactor is at a steady-state, what is the volumetric oxygen transfer coefficient (kLa)? b. The specific OUR of myeloma cells is 5*10-11 mmol/cell*h. What is the cell concentration? c. What is the respiratory quotinent (RQ) (i.e., mole CO2 produced/mol O2 consumed)? Assume that the mole fraction of CO2 in the ambient air is negligible. The universal gas constant R = 0.082 L*atm/mol*KA 10-L stirred tank is used to cultivate mouse myeloma cells. Dry air is sparged from the bottom at 1.0 L/min to supply oxygen to the system. The gas composition at the outler is 16% oxygen, 3.5% CO2, 6.5% water vapor, and balanced nitrogen. The ambient pressure and temperature are 1 atm and 37 °C, respectively. The dissolved oxygen in the reactor is controlled at 20% of saturation with ambient air. What is the respiratory quotinent (RQ) (i.e., mole CO2 produced/mol O2 consumed)? Assume that the mole fraction of CO2 in the ambient air is negligible. The universal gas constant R = 0.082 L*atm/mol*K
- 45. What happens to CO2 levels with increasing sugar concentration? Is this pattern observed for all sucrose treatments? 46. Based on your results, can you explain which ingredients are essential for fermentation to occur and why? 47. Did your observed results reflect your predictions for the circumference of the balloon? 48. Do you reject or fail to reject your null hypothesis for the circumference of the balloon? Explain. 49. Did your observed results reflect your predictions for the percentage of alcohol? 50. Do you reject or fail to reject your null hypothesis for the percentage of alcohol? Explain. 51. Identify any possible sources of error that may have affected the results of this experiment. 52. Using Excel, make a bar graph with the results of the circumference of the balloon and the percentage of alcohol side by side.During fermentation, bubbles can be seen emerging from the glass tubing. Identify the gas causing the bubbles.The following data are given for a biogas digester suitable for the output of six cows. The volume of digester is 7.4 m3, the volume of gas holder is 2.9 m and the retention time is 21 days. Find the height of gas holder cm height of gas holder =-
- Lab students placed ten spinach leaf disks in each of three test tubes containing a baking soda solution and a control tube without baking soda. The volume of liquid in each tube and the temperature of the solutions were held constant. All tubes were placed an equal distance from a light source as indicated in the accompanying table. After 10 minutes, the amount of oxygen present in each sample was measured. Test Tube Grams of Baking Soda (CO2 source) Wattage of Light Bulb 1 0.5 25 2 0.5 50 3 0.5 75 4 0 75 Which tube is expected to contain the greatest quantity of oxygen? Tube 1 Tube 2 Tube 3 Tube 4Lab students placed ten spinach leaf disks in each of three test tubes containing a baking soda solution and a control tube without baking soda. The volume of liquid in each tube and the temperature of the solutions were held constant. All tubes were placed an equal distance from the light source indicated in the accompanying table. After 10 minutes, the amount of oxygen present in each sample was measured. Test Tube Grams of Baking Soda (CO2 source) Wattage of Light Bulb 1 0.5 25 2 0.5 50 3 0.5 75 4 0 75 Which tube is expected to produce the least amount of oxygen? a) Tube 1 b) Tube 2 c) Tube 3 d) Tube 4A glucose solution is supplied in a repeated fed batch culture. The following parameters have been measured at t=2.5 hours; the system was under a quasi-steady state at this time. V = 1.0L, F = 0.22L/hr, S_0 = 98g/L, mu_m = 0.31/hr, K_s = 0.14 g-glucose/L, YM_X/S = 0.48 g-cells/g-glucose, Xt_0 = 33g (1) Calculate the value of V0 and S. (2) Calculate the value of Xt for t = 2.5hrs.
- Given: 0.6 μM h1 NO3 remineralization Calculate: µM h1 CO₂ respiration AA microorganism is grown in a fermenter under steady-state conditions. The microorganism growth follows Monod kinetics and the following stoichiometric equation: C3H6O3 +a O2 +b NH3 → c biomass + d CO2 + e H₂O Given that the biomass yield is 0.415 grams of biomass per gram of substrate, determine the respiratory quotient.Below is a simple set of weights obtained while immersing potato slices in various sugar solutions over 30 minutes. For each solution, calculate the rate of weight change over this 30-minute period. standard notation and use 3 digits past the decimal point 0.0 M sucrose = ____________________ g/min 0.4 M sucrose = _____________________ g/min 0.6 M sucrose = _____________________ g/min 0.8 M sucrose = _____________________ g/min 1.0 M sucrose = _____________________ g/min Using the osmosis data in the question above, determine the percent weight change for each sucrose solution standard notation and use 1 digit past the decimal point - Show your work in the space below. 0.0 M sucrose = ____________________% 0.4 M sucrose = _____________________% 0.6 M sucrose = _____________________% 0.8 M sucrose = _____________________% 1.0 M sucrose = _____________________% Time (min) 0M sucrose 0.4M sucrose 0.6M sucrose 0.8 M sucrose 1.0M sucrose 0 4.36 g…