=20 nM. The rate of receptor-ligand complex formation with anA receptor-ligand complex has a dissociation constant of Kaadded ligand concentration of 10 µM is 5 × 10³ s¯¹. What is the value of the reverse rate constant, k_₁ ?k_₁=8-1

The dissociation constant (Kd) for a receptor-ligand complex is related to the rate constants for…

A receptor-ligand complex has a dissociation constant of Kd = 20 nM. The rate of receptor-ligand complex formation with an added ligand concentration of 10 µM is 5 × 10³ s-¹. What is the value of the reverse rate constant, k_₁?

A receptor-ligand complex has a dissociation constant of Kd = 20 nM. The rate of receptor-ligand complex formation with an added ligand concentration of 10 µM is 5 × 10³ s-¹. What is the value of the reverse rate constant, k_₁?

Biochemistry

9th Edition

ISBN:9781319114671

Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Chapter1: Biochemistry: An Evolving Science

Section: Chapter Questions

Problem 1P

See similar textbooks

Related questions

Q: Consider a uniport system where a carrier protein transports an uncharged substance A across a cell…

A: ∆G = R T ln (Ainside /Aoutside) Where G is free energy change for the transport of uncharged…

Q: Which of the following must be true based on the data below for ligand L binding to protein A and to…

A: Proteins are biomolecules which catalyzes most of the biochemical reactions in all living cells and…

Q: (d) ( through the disulfide bridge. If this bridge were found for the two joined amino acids free in…

A: Proteins are composed of amino acids, which are bound together by peptide linkage. Amino acids…

Q: the reduced electron carrier that enters Complex II of the electron transport chain, downstream of…

A: Energy is needed by the cell to do work. The energy is produced by oxidizing the glucose molecules…

Q: Two co-op students at your start-up company have been asked to evaluate the rate of reaction…

A: An enzyme is a cellular catalyst that speeds up chemical reactions. Proteins that bind to…

Q: Human serum transferrin is an iron transport protein. With the use of appropriate diagrams, describe…

A: Transferrin protein is a protein complex, which is known to transfer iron molecules to the cell.

Q: 2+ (aq) Cd(s) 1. Pbaq) + Cdag) +

A: You have asked multiple questions. I will be answering the first question. Pb2+(aq.) + Cd(s) gives…

Q: The figure shows binding curves for two proteins that bind the same ligand. Which binding curve…

A: Ligand binding curve: this is also called as saturation curve that shows the fraction Y of protein…

Q: The Structure of the acetylcholine receptor is shown below: A. Knowing the amino acid sequence of…

A: All amino acids are of different property based on their property they present on the cytoplasmic…

Q: Molecular diameters of proteins IgG and TNFα are 11.2 nm and 4.4 nm, respectively. The diffusion…

A: Stokes-Einstein equation is the relationship between radius and diffusion coefficient of a substance…

Q: 1) How many binding sites per molecule does the receptor have? 2) What type of cooperativity is…

A: Binding sites It is a location on a protein known as a ligand that attaches to an incoming molecule…

Q: Given the melting profile of a membrane bilayer consisting of (see attached diagrams) where R =…

A: The graph here has degree of fluidity as Y-axis and temperature as X-axis. The curve will shift to…

Q: 1) A ligand-binding protein showing negative homotropic cooperativity? a) should give an nH value…

A: Cooperativity or cooperative binding occurs when binding of one molecule influences the affinity of…

Q: the number of low affinity binding sites occupied by a ligand at 100% saturation is greater than the…

A: Introduction A molecule that particularly attaches to another molecule, often a bigger one, is known…

Q: To better understand how the lacR works, let's start let's approximate the volume of a bacterial…

A: The bacterial cell is considered as a cylInder. The equation to find volume of cylinder is ''.

Q: The fatty acid side chains of the phospholipids in the inner mitochondrial membrane exhibit a large…

A: Since you have posted multiple questions, we will provide the solution only to the first question as…

Q: You've got a solution containing 60 μM VIP (very important protein). VIP binds to its ligand L with…

A: Ligand-protein interactions are best described by their Dissociation Constants (KD). Let us…

Q: Given a tripeptide Cys-His-Lys, Cys: Pk1 = 1.71; Pk2 = 10.78; PkR = 8.33 His: Pk1 =…

A: Hi. Thank you for the question, As per the honor code, we are allowed to answer three sub-parts at…

Q: Why is COVID-19 a man made? (Brief explanation only atleast 3-5 sentences)

A: Coronavirus is large family of virus that are the source of respiratory infection, including the…

Q: Which ligand-binding curve in the figure below would be consistent with the protein exhibiting a Ka…

A: Consider the interaction between a protein (P) and ligand (L) as shown below.Here, Ka is the…

Q: Crystal structures exist for three neurokinin-1 (NK1) ligand complexes with the following pdb codes…

A: NK1 is a type of mammalian tachykinin receptors. These are G-protein coupled receptors which are…

Q: centration of 0.05 mg/L. The cancer potency factor for ingesting benzene is 2.9 x 102 mg/kg.day. a)…

A: Answer ::

Q: An increase in PCO2 will result in which of the following? O a) An increased affinity between PO,…

A: Haemoglobin oxygen dissociation curve is a sigmoidal curve. A shift of the curve to the right…

Q: The

A: Introduction: The nervous system, a complex network of specialized neurons and supporting cells…

Q: Can you explain the experimental procedure from this article? Please explain as if your having…

A: In this work, the role of inositol 1,4,5-trisphosphate receptor (InsP3R) and Na, K-ATPase in the…

Q: Members of the integrin family are the major cell surface receptors for many ECM proteins such as…

A: Integrins are a family of αβ heterodimers. Integrins are cellular adhesion receptor molecules that…

Q: Cadherins are known to mediate homophilic interactions between cells. What is a homophilic…

A: Cadherins are types of cell adhesion molecules (CAM) these are important in the formation of adheren…

Q: The following data describe binding of ligand A to a protein, using both Scatchard plots and Hills…

A: Scatchard plots is used to determine the affinity of the receptor or any protein for its ligand and…

Q: A new protein namely protein X has been isolated by molecular biologists. The protein is shown to…

A: Protein: Proteins are necessary nutrients for human health. They are a component of body tissue and…

Q: The alpha subunit of the heterotrimeric G-protein complex becomes activated upon activation of a…

A: Introduction: G Protein-coupled receptor (GPCR) also known as serpentine receptors or seven-pass…

Q: Give an example of a protein containing primarily betapleated sheets. Is this a fibrous or globular…

A: Protein structure decides the amino acid sequence, chemical bonds found between atoms in the…

Q: Hormone A binds to its receptor with a KL of 5 μM. In the presence of 2.5 μM compound B and 2.0 μM…

A: A ligand is a substance that forms a complex with a biomolecule to serve a biological purpose. In…

Q: Substrate A has a Km of 45uM and a kcat of 100/s with trypsin and a Km of 540mM and a kcat of 2/s…

A: Enzymes are the proteinous structure that helps in increasing the rate of metabolism. The function…

Q: In a Myoglobin and azide ligand-receptor binding experiment, instead of using 3.5 µM myoglobin you…

A: Myoglobin acts as oxygen reserve in the muscle cells. Myoglobin (Mb) has higher affinity towards…

Q: Given that the protein concentration is 0.25 μM, what is the association constant (KA) of the…

A: Fluorescence is a luminescence technique used in fluorescent labeling and fluorescence spectroscopy…

Q: The toxic effect of Na+ ions can be avoided in some plant species by storing them in membrane-bound…

A: One kind of active transport that moves Na+ ions through a membrane is the Na+/H+ antiport…

Q: Dr Merrick is so excited to know the affinity constant Kd values for O2 binding to mammoth OBP and…

A: ANSWER;- It is given that the ka values of OBP and elephants Hb are the same at pH=7. Kd is the…

Q: A galvanic cell at a temperature of 25.0 °C is powered by the following redox reaction: 2+ 2+ Sn(aq)…

A: To calculate cell voltage of galvanic cell under given conditions , we need to use Nernst…

Q: Estimate the binding affi nity of a ligand for its receptor from the followingdata:

A: The receptor is a protein that is found on the cell surface. These receptors bind to the ligand with…

Q: An antibody binds to another protein with anequilibrium constant, K, of 5 × 109 M–1. When it binds…

A: The equilibrium constant, K, expresses the relationship between a reaction's products and reactants…

Q: Provide a brief explanation as to why ligand binding to proteins must be a reversible process.

A: A protein–ligand complex is a complex made up of a protein and a ligand that forms as a result of…

Q: Calculate the solubility of Ag,CO3 (in mol/L) at 25 °C in a 0.02 M Na,CO3 solution Hint: Ag,CO3(s) →…

A: Solubility equilibrium is a type of dynamic equilibrium that exists when a chemical compound in the…

Question

=
20 nM. The rate of receptor-ligand complex formation with an
A receptor-ligand complex has a dissociation constant of Ka
added ligand concentration of 10 µM is 5 × 10³ s¯¹. What is the value of the reverse rate constant, k_₁ ?
k_₁
=
8-1

Expert Solution

This question has been solved!

Explore an expertly crafted, step-by-step solution for a thorough understanding of key concepts.

This is a popular solution!

SEE SOLUTION Check out a sample Q&A here

Step 1: Dissociation constant (Kd)

VIEW

Step 2: Conversion of unit from nM to M

VIEW

Solution

VIEW

Trending now

This is a popular solution!

Step by step

Solved in 3 steps

SEE SOLUTION Check out a sample Q&A here

Follow-up Questions

Read through expert solutions to related follow-up questions below.

Follow-up Question

added ligand concentration of 10 µM is 5 × 10³ s¹. What is the value of the reverse rate constant
k_1
=
1 ×10-4
Incorrect
Macmillan Learning
You have not correctly calculated the
value of the reverse rate constant.
To begin, calculate the value of k₁ by
dividing the rate of receptor-ligand
complex formation (the rate of the
forward reaction) by the
ligand concentration.
k₁
=
rate forward
[L]
Then, calculate the value of the reverse
rate constant, k_₁, by multiplying the
dissociation constant, Kd, and the
forward rate constant, k₁.
k_1 = Kak₁

Solution

by Bartleby Expert

SEE SOLUTION

Similar questions

What effect does a negative effector have on the graph of reac- tion rate (V) vs. [substrate] for an allosteric effector?
During an SAR effort to identify tyrosine kinase inhibitors, it was found that compound 2 was significantly more potent and selective than compound 1. To what might you attribute this improvement in activity and selectivity? Explain. IC-5 micromolar IC-0.1 mromolar
Given a tripeptide Cys-His-Lys, Cys: Pk1 = 1.5; Pk2 = 10.8; PkR = 8.5 His: Pk1 = 1.6; Pk2 = 9.0; PkR = 7.0 Lys: Pk1 = 2.2; Pk2 = 8.5; PkR = 9.8 a.draw the protonic equilibria for the tripeptide. b.what is the IpH? c.What is the dominant structure at pH 3.0? d.What is the first buffering region of the tripeptide?
In a molecular disease of hemoglobin, Hemoglobin Rainier, Tyr 145β is replaced by Cys, which forms a disulfide bond with another Cysresidue in the same subunit. This prevents the formation of ion pairs that normally stabilize the T state. How does hemoglobin Rainier differ from normal hemoglobin with respect to the Hill coefficient
A sample of a protein is analyzed by CE using a neutral-coated capillary with a total length of 25.0 cm and a distance to the detector of 22.0 cm. Using an applied voltage of 20.0 kV, the protein was detected at a migration time of 16.2 min. The diffusion coefficient for the protein is 2.4 x 10-7 cm?/s. (a) Calculate the apparent electrophoretic mobility of the protein. (b) Calculate the expected number of theoretical plates for the analysis.
If instead of using 3.5 µM myoglobin (receptor) you used half of this (that is, 1.75 µM myoglobin), what would be that value of the Kd, that you calculated ( how would it change)? Please explain so I can solve on my own :) (How does changing concentration of the receptor in a ligand-receptor binding experiment affect the dissociation constant?)
A one-to-one protein (P)-ligand (L) complexation (P + L PL) has a dissociation equilibrium constant (Kd) value of 100 nM at 25°C, and the Kd remains the same at 37°C. 1) What is AS of binding at 25°C? Assume ACp of the binding is 0 over the temperature range. AS = 1.34E2 kJ/(mol*K) (note the unit!!) (sig. fig =3) 2) What is the concentration of the PL complex formed at equilibrium when you mix 0.20 uM (microM) of Protein and 1.0 uM of Ligand together at 37°C? PL at equilibrium = 8.1E-1 uM (note the unit!!) (sig. fig =2)
Two proteins, A and B, bind to the same ligand, L, with the binding curvesshown below. What is the dissociation constant, Kd , for each protein? Which protein (A or B) has a greater affinity for ligand L?
List three examples of commonly employed combination of ligand and protein in affinity chromat
The affinity of hemagglutinin for sialic acid is quite low, with a Kd of approximately 5 mM. How does efficient attachment occur with such a low-affinity interaction?
Explain the use of passing the inside-out vesicles contaminated with right-side-out vesicles through the affinity column made of lectin coupled to solid beads.
List 4 different actiated carrier molecules, for each one, list both the oxided and reduced forms