=20 nM. The rate of receptor-ligand complex formation with anA receptor-ligand complex has a dissociation constant of Kaadded ligand concentration of 10 µM is 5 × 10³ s¯¹. What is the value of the reverse rate constant, k_₁ ?k_₁=8-1

The dissociation constant (Kd) for a receptor-ligand complex is related to the rate constants for…

A receptor-ligand complex has a dissociation constant of Kd = 20 nM. The rate of receptor-ligand complex formation with an added ligand concentration of 10 µM is 5 × 10³ s-¹. What is the value of the reverse rate constant, k_₁?

A receptor-ligand complex has a dissociation constant of Kd = 20 nM. The rate of receptor-ligand complex formation with an added ligand concentration of 10 µM is 5 × 10³ s-¹. What is the value of the reverse rate constant, k_₁?

Biochemistry

9th Edition

ISBN:9781319114671

Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Chapter1: Biochemistry: An Evolving Science

Section: Chapter Questions

Problem 1P

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Question

=
20 nM. The rate of receptor-ligand complex formation with an
A receptor-ligand complex has a dissociation constant of Ka
added ligand concentration of 10 µM is 5 × 10³ s¯¹. What is the value of the reverse rate constant, k_₁ ?
k_₁
=
8-1

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Step 1: Dissociation constant (Kd)

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Step 2: Conversion of unit from nM to M

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Follow-up Questions

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Follow-up Question

added ligand concentration of 10 µM is 5 × 10³ s¹. What is the value of the reverse rate constant
k_1
=
1 ×10-4
Incorrect
Macmillan Learning
You have not correctly calculated the
value of the reverse rate constant.
To begin, calculate the value of k₁ by
dividing the rate of receptor-ligand
complex formation (the rate of the
forward reaction) by the
ligand concentration.
k₁
=
rate forward
[L]
Then, calculate the value of the reverse
rate constant, k_₁, by multiplying the
dissociation constant, Kd, and the
forward rate constant, k₁.
k_1 = Kak₁

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