Add the following to 400ml H2O (not from the tap!)   5.4 g Tris base 50.0ml 10X Boric acid (55.0 mg/ml) 2.0ml 0.5M EDTA solution   Adjust the final volume to 500ml with additional H2O, transfer to a bottle and label it as you will need this next week. Calculate the molarity of the tris, boric acid and EDTA in the 1X solution (to 2 decimal places).

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