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MW (g/mol)-137.99 Buffer 1: 1 M NaH₂PO V 100 mL m(g) Buffer 2: 1 M Na2HPO4 V-100 mL MW (g/mol) 142 m(g) Buffer 3: 100 mM Sodium Phosphate buffer V= 100 mL Volume (mL) of 1 M NaH₂PO4 Volume (mL) of 1 M Na₂HPO4 Table 1 Buffer 4: 1.5 M Tris, pH 8.8 MW (g/mol)-121.14 Buffer 5: 0.5 M Tris, pH 6.8 MW (g/mol)=121.14 MW Tris (g/mol) = 121.14 MW Glycine (g/mol) = 75.07 MW SDS (g/mol) = 288.38 m(g) = MW (g/mol) = 121.14 MW Glycine (g/mol) = 75.07 V= 100 mL Buffer 6: 10X SDS Running Buffer, pH 8.3 V= 100 mL m(g) m(g) = m(g) = V100 mL Buffer 7: 10X Native Running Buffer, pH 8.3 m(g) m(g) = PRELAB V= 100 mL

MW (g/mol)-137.99 Buffer 1: 1 M NaH₂PO V 100 mL m(g) Buffer 2: 1 M Na2HPO4 V-100 mL MW (g/mol) 142 m(g) Buffer 3: 100 mM Sodium Phosphate buffer V= 100 mL Volume (mL) of 1 M NaH₂PO4 Volume (mL) of 1 M Na₂HPO4 Table 1 Buffer 4: 1.5 M Tris, pH 8.8 MW (g/mol)-121.14 Buffer 5: 0.5 M Tris, pH 6.8 MW (g/mol)=121.14 MW Tris (g/mol) = 121.14 MW Glycine (g/mol) = 75.07 MW SDS (g/mol) = 288.38 m(g) = MW (g/mol) = 121.14 MW Glycine (g/mol) = 75.07 V= 100 mL Buffer 6: 10X SDS Running Buffer, pH 8.3 V= 100 mL m(g) m(g) = m(g) = V100 mL Buffer 7: 10X Native Running Buffer, pH 8.3 m(g) m(g) = PRELAB V= 100 mL

Chemistry
Chemistry
10th Edition
ISBN:9781305957404
Author:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste
Publisher:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste
Chapter1: Chemical Foundations
Section: Chapter Questions
Problem 1RQ: Define and explain the differences between the following terms. a. law and theory b. theory and...
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Buffer 1: 1 M NaH₂PO4 V 100 mL m(g): MW (g/mol)-137.99 Buffer 2: 1 M Na2HPO4 V-100 mL MW (g/mol) 142 Volume (mL) of 1 M NaH₂PO4 Volume (mL) of 1 M Na2HPO4 Buffer 3: 100 mM Sodium Phosphate buffer V= 100 mL Table 1 MW (g/mol)-121.14 Buffer 4: 1.5 M Tris, pH 8.8 MW (g/mol)=121.14 m(g) = Buffer 5: 0.5 M Tris, pH 6.8 MW Tris (g/mol) = 121.14 MW Glycine (g/mol) = 75.07 MW SDS (g/mol) = 288.38 MW (g/mol) = 121.14 MW Glycine (g/mol) = 75.07 m(g) = V= 100 mL Buffer 6: 10X SDS Running Buffer, pH 8.3 V= 100 mL m(g) = m(g) = m(g) = V= 100 mL Buffer 7: 10X Native Running Buffer, pH 8.3 m(g) = m(g) = PRELAB V= 100 mL
Transcribed Image Text:Buffer 1: 1 M NaH₂PO4 V 100 mL m(g): MW (g/mol)-137.99 Buffer 2: 1 M Na2HPO4 V-100 mL MW (g/mol) 142 Volume (mL) of 1 M NaH₂PO4 Volume (mL) of 1 M Na2HPO4 Buffer 3: 100 mM Sodium Phosphate buffer V= 100 mL Table 1 MW (g/mol)-121.14 Buffer 4: 1.5 M Tris, pH 8.8 MW (g/mol)=121.14 m(g) = Buffer 5: 0.5 M Tris, pH 6.8 MW Tris (g/mol) = 121.14 MW Glycine (g/mol) = 75.07 MW SDS (g/mol) = 288.38 MW (g/mol) = 121.14 MW Glycine (g/mol) = 75.07 m(g) = V= 100 mL Buffer 6: 10X SDS Running Buffer, pH 8.3 V= 100 mL m(g) = m(g) = m(g) = V= 100 mL Buffer 7: 10X Native Running Buffer, pH 8.3 m(g) = m(g) = PRELAB V= 100 mL
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