acute T cell leukemia. K562 cells were the first human immortalized myelogenous leukemia cell line to be established. Resting= control, Etoposide= drug. Below is shown the results of floweytometry of untreated and treated cells. a Does Etoposide have an effect on Jurkat cells? K562 cells? b. Does the mechanism of action of Etoposide appear to be necrosis or apoptosis?
acute T cell leukemia. K562 cells were the first human immortalized myelogenous leukemia cell line to be established. Resting= control, Etoposide= drug. Below is shown the results of floweytometry of untreated and treated cells. a Does Etoposide have an effect on Jurkat cells? K562 cells? b. Does the mechanism of action of Etoposide appear to be necrosis or apoptosis?
Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
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Question
![A) Resting Jurkat cells
1 Dead
B) Jurkatcells-Etoposide
Dead
0:29
Live
APO
Live
АРО
571
10
Annexin V inoA
10
10
Annexin VitcA
C) Resting K562 cells
14.86
Dead
D) K562 cells-Etoposide
fo se
Dead
114
Live
APO
Live
АРО
10
onexin VncA
Annexin V FincA
Legend. Jurkat & K562 cels were incubated with 10uM etoposide for 24h. Resting
Jurkat cells A), etoposide treated Jurkat cells B), resting K662 cells C) etoposide
treated K562 cells D) were labelled with annexin V-FITC and DAPI (200ng/mi).
Courtesy of the Flow Cytometry Core Facility, Blizard Institute, Barts & The London
School of Medicine & Dentistry, Queen Mary University of London, E1 2AT.
DAP
DAP
DAP](/v2/_next/image?url=https%3A%2F%2Fcontent.bartleby.com%2Fqna-images%2Fquestion%2F1e3c2ed7-c2e8-47d1-88b7-cda6e6b047ba%2F50796b39-9c5d-459d-bdf8-1b2810580d53%2F01slxqs_processed.jpeg&w=3840&q=75)
Transcribed Image Text:A) Resting Jurkat cells
1 Dead
B) Jurkatcells-Etoposide
Dead
0:29
Live
APO
Live
АРО
571
10
Annexin V inoA
10
10
Annexin VitcA
C) Resting K562 cells
14.86
Dead
D) K562 cells-Etoposide
fo se
Dead
114
Live
APO
Live
АРО
10
onexin VncA
Annexin V FincA
Legend. Jurkat & K562 cels were incubated with 10uM etoposide for 24h. Resting
Jurkat cells A), etoposide treated Jurkat cells B), resting K662 cells C) etoposide
treated K562 cells D) were labelled with annexin V-FITC and DAPI (200ng/mi).
Courtesy of the Flow Cytometry Core Facility, Blizard Institute, Barts & The London
School of Medicine & Dentistry, Queen Mary University of London, E1 2AT.
DAP
DAP
DAP
![Jurkat cells are an immortalized line of human T-lymphocytes cells that are used to study
acute T cell leukemia. K562 cells were the first human immortalized myelogenous
leukemia cell line to be established. Resting= control, Etoposide= drug. Below is shown
the results of floweytometry of untreated and treated cells.
a. Does Etoposide have an effect on Jurkat cells? K562 cells?
b. Does the mechanism of action of Etoposide appear to be necrosis or apoptosis?](/v2/_next/image?url=https%3A%2F%2Fcontent.bartleby.com%2Fqna-images%2Fquestion%2F1e3c2ed7-c2e8-47d1-88b7-cda6e6b047ba%2F50796b39-9c5d-459d-bdf8-1b2810580d53%2Fzg82fbs_processed.jpeg&w=3840&q=75)
Transcribed Image Text:Jurkat cells are an immortalized line of human T-lymphocytes cells that are used to study
acute T cell leukemia. K562 cells were the first human immortalized myelogenous
leukemia cell line to be established. Resting= control, Etoposide= drug. Below is shown
the results of floweytometry of untreated and treated cells.
a. Does Etoposide have an effect on Jurkat cells? K562 cells?
b. Does the mechanism of action of Etoposide appear to be necrosis or apoptosis?
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