You then conduct a second experiment.  Wild-type and C3 deficient mice are each inoculated with a replication-defective form of HSV (called HSV-rd). This virus infects cells but is not able to replicate and spread in the body.   For this experiment, mice are infected with varying doses of the HSV-rd virus, and peak IgG responses to the viral surface glycoproteins are measured. The results are shown in Figure 2.

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Viral Replication

The virus is acellular and considered non-living outside the host cell. The replication of the virus occurs only inside the living cells. Only a few genes are present in the smallest virus, whereas many genes are present in the largest virus. These genes encode specific viral proteins.