Absorbances of a series of phosphate standard solutions measured using UV- Vis spectrophotometer using 1.0 cm pathlength glass cuvette at 415 nm are given in table 2 below. Table 2 Standard solution Concentration (mmol/L) Absorbance Std 1 Std 2 0.0 0.1 0.15 Std 3 Std 4 0.2 0.3 0.28 0.4 Std 5 0.4 0.55 0.7 Std 6 0.5 If an unknown sample exhibits an absorbance of 0.45. Determine the phosphate concentration in this unknown sample solution, using: a) Calibration plot b) Least-square method
Absorbances of a series of phosphate standard solutions measured using UV- Vis spectrophotometer using 1.0 cm pathlength glass cuvette at 415 nm are given in table 2 below. Table 2 Standard solution Concentration (mmol/L) Absorbance Std 1 Std 2 0.0 0.1 0.15 Std 3 Std 4 0.2 0.3 0.28 0.4 Std 5 0.4 0.55 0.7 Std 6 0.5 If an unknown sample exhibits an absorbance of 0.45. Determine the phosphate concentration in this unknown sample solution, using: a) Calibration plot b) Least-square method
Chemistry
10th Edition
ISBN:9781305957404
Author:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste
Publisher:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste
Chapter1: Chemical Foundations
Section: Chapter Questions
Problem 1RQ: Define and explain the differences between the following terms. a. law and theory b. theory and...
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