Absorbances of a series of phosphate standard solutions measured using UV- Vis spectrophotometer using 1.0 cm pathlength glass cuvette at 415 nm are given in table 2 below. Table 2 Standard solution Concentration (mmol/L) Absorbance Std 1 Std 2 0.0 0.1 0.15 Std 3 Std 4 0.2 0.3 0.28 0.4 Std 5 0.4 0.55 0.7 Std 6 0.5 If an unknown sample exhibits an absorbance of 0.45. Determine the phosphate concentration in this unknown sample solution, using: a) Calibration plot b) Least-square method

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Absorbances of a series of phosphate standard solutions measured using UV-
Vis spectrophotometer using 1.0 cm pathlength glass cuvette at 415 nm are
given in table 2 below.
7.
Table 2
Standard solution
Concentration (mmol/L)
Absorbance
Std 1
0.0
0.1
Std 2
0.15
Std 3
0.2
0.28
Std 4
0.3
0.4
Std 5
0.4
0.55
Std 6
0.5
0.7
If an unknown sample exhibits an absorbance of 0.45. Determine the
phosphate concentration in this unknown sample solution, using:
a)
Calibration plot
b)
Least-square method
Transcribed Image Text:Absorbances of a series of phosphate standard solutions measured using UV- Vis spectrophotometer using 1.0 cm pathlength glass cuvette at 415 nm are given in table 2 below. 7. Table 2 Standard solution Concentration (mmol/L) Absorbance Std 1 0.0 0.1 Std 2 0.15 Std 3 0.2 0.28 Std 4 0.3 0.4 Std 5 0.4 0.55 Std 6 0.5 0.7 If an unknown sample exhibits an absorbance of 0.45. Determine the phosphate concentration in this unknown sample solution, using: a) Calibration plot b) Least-square method
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