Ab 540 nm Figure 1 0.6 0.5 0.4 0.3 0.2 0.1 0 0 0.1 0.2 0.3 0.4 0.5 0.6 0.7 0.8 concentration g/L

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A 0.5 ml sample of the stock haemoglobin (question 3) was mixed with 10 mls of Cyanmet reagent, left at room temperature and an absorbance reading of 0.25 was obtained at 540 nm. Using the standard curve for cyanmethaemoglobin in Figure 1, estimate the concentration of haemoglobin in your stock solution. The concentration of hemoglobin should be computed in mg/ml Figure 1 Ab 540 nm 0 0.1 02 06 07 08 03 04 concentration 05 L 1.0.175 2.0.375 3.3.50 4.7.50 5.0.75 

Ab 540 nm
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Transcribed Image Text:Ab 540 nm Figure 1 0.6 0.5 0.4 0.3 0.2 0.1 0 0 0.1 0.2 0.3 0.4 0.5 0.6 0.7 0.8 concentration g/L
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