Question one parts A though ea. You have a plan to make the cellular signaling peptide RGD (Arg-Gly-Asp). If you are beginning fromthe Arginine (Arg) amino acid, which amino acid(s) will require NH2 protection to optimize thesynthesis?A) All 3 amino acids will need NH2 protectionB) Gly and Asp will need NH2 protectionC) Arg and Gly will need NH2 protectionD) Only Argb. Which of the following amino acids will not produce a purple color (indicative of 2 ninhydrinmolecules binding) when reacted with ninhydrin?A) phenylalanineB) valineC) prolineD) histidinec. If you had a mixture of leucine, arginine, and glutamic acid in a pH of 6 and applied a drop onto paperconnected to an electrophoresis apparatus. Which of these would move toward the cathode (-).A) LeucineB) ArginineC) Glutamic AcidD) None of themd. True/False - Dicyclehexvlcarbodiimide is a typical reagent in peptide synthesis for protectingcarboxylic acid groups.e. True/False t-butoxycarbonyl dicarbonate (Boc20) adds a t-butoxy carbonyl group to the amineportion of the amino acid creating an amide (carbamate) and protects the amine portion from sidereactions.

Answered: Image /qna-images/answer/94e192a6-82b6-4063-9620-b7f87ae34a9b.jpg

Answered: a. You have a plan to make the cellular…

Biochemistry

9th Edition

ISBN:9781319114671

Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Chapter1: Biochemistry: An Evolving Science

Section: Chapter Questions

Problem 1P

See similar textbooks

Similar questions

The transforming growth factory beta (TGF-b) family of hormones / growth factors are dimers and they interact with specific receptors. Which of the following statements about these receptors is correct? a) A receptor complex is formed from a type 1 and a type 2 receptor b) A receptor complex is formed between the ligand and the receptor c) This family interacts with a single serine- threonine kinase receptor d) The major receptor is intracellular e) The receptor is a G-protein receptor
What type of mutation might render the following proteins constitutively ac- tive: (a) Smad3, (b) MAP kinase, and (c) NF-κB?
b) In eukaryotes, the newly synthesized MRNA undergoes modifications before it is transported across the nuclear membrane into the cytoplasm. Outline any two of the three modifications.
1) The plot to the right shows the Oxygen Saturation of Hemoglobin (Hb) versus O2 concentration. a) The Kd between HBa and HBB chains is 100 nM. When L35S mutation (Leucine 35 → Serine) is introduced into the Hba chain the Kd changes to 300 mm. \^^^^^A^A Draw a dashed curve on the figure to the right that represents how this mutation would likely change the curve. Note: that I am asking for how a change in affinity between HBa and HBB chains will impact HB binding to O2 1.00 0.75- HB Y 0.50 HBa L35S HBa L35S D101P 0.25 0.00 0 2 4 po₂ (kPa) 6 b) How might this specific mutation alter the interaction between HBa and HBB chains? I am asking you to predict what Leu specifically might be doing here. 8 c) Now imagine that a second mutation is added to the HB¤ L35S mutant to generate an HBa L35S D101P double mutant. A measure of the double mutant shows that it has a Kg = 2 kPa, but does not change the binding of HBa and HBB. Draw a dotted line on the figure above that shows the resulting…
Transcription and translation are separate processes in gene expression; however, they have similarities. The following terms all relate to translation. Which of these has a role that is most similar to that of the transcription start site during transcription? A)Start codon B)Stop codon C)tRNA D)Amino acid
We learned that three different amino acid transformations of PLP-dependent enzymes canresult from different conformations of the PLP-amino acid imine adduct in the active site.Starting from the PLP adduct of (S)-serine, show mechanisms fora) Decarboxylation of serineb) Racemization of serinec) Conversion of serine to glycine and formaldehyde.
please answer the full question
You plan to synthesize a peptide to be used as a vaccine to treat melanoma, a particularly aggressive form of skin cancer. Normally, gp100, a protein on the surface of melanocytes, activates cell growth when it is bound by its ligand. Activation of the growth pathway depends on the presence of threonine in the ligand. The effective peptide vaccine will mimic the natural ligand, but won’t cause cell growth and division. Below is the sequence of the natural ligand: LDMKTAG In order to ensure your newly designed peptide vaccine does not cause cell growth upon binding, you must substitute the Threonine residue at position 5. What amino acid would you replace it with, bearing in mind that the peptide should still be similar enough to bind to the gp100 protein in the surface of melanocytes. Explain your choice. Your vaccine will be administered as a topical cream, and you require your peptide to have an overall neutral charge in order to be functional. At what pH should you formulate…
You are studying a eukaryotic protein PKK in the lab. You fuse the DNA encoding GFP (green fluorescent protein) to the C terminus of the PKK gene. You also create the following GFP-PKK variants -GFP Wild- type: N- Signal sequence Transmembrane domain Kinase domain Variant 1: N- GFP Transmembrane domain Kinase domain Variant 2: N- -GFP Signal sequence Transmembrane domain Variant 3: N- -GFP Signal sequence Kinase domain Variant 4: N- -GFP Kinase domain If you examine wild-type PKK trafficking in a eukaryotic cell, which of the following are true? Select all that apply This fusion protein will be trafficked to the nucleus This fusion protein is a cell membrane protein This fusion protein will be trafficked to the Endoplasmic Reticulum This fusion protein is a nuclear protein
For each of the following situations, provide a plausible explanation for how it could lead to unrestricted cell division.(a) Colon cancer cells often contain mutations in the gene encoding the prostaglandin E2 receptor. PGE2 is a growth factor required for the division of cells in the gastrointestinal tract.(b) Kaposi sarcoma, a common tumor in people with untreated AIDS, is caused by a virus carrying a gene for a protein similar to the chemokine receptors CXCR1 and CXCR2. Chemokines are cell-specific growth factors.(c) Adenovirus, a tumor virus, carries a gene for the protein E1A, which binds to the retinoblastoma protein, pRb. (d) An important feature of many oncogenes and tumor suppressor genes is their celltype specificity. For example, mutations in the PGE2 receptor are not typically found in lung tumors. Explain this observation. (Note that PGE2 acts through a GPCR in the plasma membrane.)
See figure 12.16b regarding the process by which cyclin regulates the Cdk. Suppose that the cyclin binding site in the Cdk contains these FOUR amino acids in this order from top to bottom: serine, lysine, aspartic acid acid and lysine and the Cdk binding site in the cyclin contains these FOUR amino acids in this order from top to bottom: aspartic acid, aspartic acid, lysine and serine. Use the schematics below to show the R groups and how they might interact to create the cyclin.cdk complex. Label both binding sites, show all charges that will be used to create any bonds, and label all bonds formed and add the ATP active site. A Explain what a kinase does and how the cyclin controls the activity of the Cdk.
A biotech company develops a biosensor that measures the presence of proteins activated by growth factor signaling cascades. Which binding domain would you use to each of the following activation peptides or signaling molecules. Phosphotyrosine A. SH2 Domain A peptide with the sequence Arg-Ala-Pro-Pro-Leu- B. Bromodomain Pro C. 14-3-3 Domain ◆ Phosphatidyl inositol Triphosphate (PIP3) Phosphoserine Acetylated Lysine D.PH Domain E. SH3 Domain

SEE MORE QUESTIONS

Recommended textbooks for you

Biochemistry

ISBN:

9781319114671

Author:

Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Publisher:

W. H. Freeman

Lehninger Principles of Biochemistry

Biochemistry

ISBN:

9781464126116

Author:

David L. Nelson, Michael M. Cox

Publisher:

W. H. Freeman

Fundamentals of Biochemistry: Life at the Molecul…

Biochemistry

ISBN:

9781118918401

Author:

Donald Voet, Judith G. Voet, Charlotte W. Pratt

Publisher:

WILEY

Biochemistry

ISBN:

9781319114671

Author:

Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Publisher:

W. H. Freeman

Lehninger Principles of Biochemistry

Biochemistry

ISBN:

9781464126116

Author:

David L. Nelson, Michael M. Cox

Publisher:

W. H. Freeman

Fundamentals of Biochemistry: Life at the Molecul…

Biochemistry

ISBN:

9781118918401

Author:

Donald Voet, Judith G. Voet, Charlotte W. Pratt

Publisher:

WILEY

Biochemistry

ISBN:

9781305961135

Author:

Mary K. Campbell, Shawn O. Farrell, Owen M. McDougal

Publisher:

Cengage Learning

Biochemistry

ISBN:

9781305577206

Author:

Reginald H. Garrett, Charles M. Grisham

Publisher:

Cengage Learning

Fundamentals of General, Organic, and Biological …

Biochemistry

ISBN:

9780134015187

Author:

John E. McMurry, David S. Ballantine, Carl A. Hoeger, Virginia E. Peterson

Publisher:

PEARSON

SEE MORE TEXTBOOKS