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A. What type of experiment is this?) A -79 CD18 Gene Promoter ccacttcctccaaggaggagctgagaggaacaggaagtgtcaggacttta cgacccgcgcctccagctgaggtttctagACGTGacccaggg B HIF-1a IP Hypoxia Cntl IP Input HIF-1 Core Site Ancillary Site Hypoxia Response Element (HRE) Normoxia HIF-la IP CAGACE +19 HIF-1 Cntl IP Input 166 bp any und oh the experiment in light of the B. Interpret information provided above. It may help to think about what the hypothesis was and the kind of information that this experiment is meant to convey. C. What can this experiment tell you about how HIF-1 or CD18 affect cell survival during stroke? -1

A. What type of experiment is this?) A -79 CD18 Gene Promoter ccacttcctccaaggaggagctgagaggaacaggaagtgtcaggacttta cgacccgcgcctccagctgaggtttctagACGTGacccaggg B HIF-1a IP Hypoxia Cntl IP Input HIF-1 Core Site Ancillary Site Hypoxia Response Element (HRE) Normoxia HIF-la IP CAGACE +19 HIF-1 Cntl IP Input 166 bp any und oh the experiment in light of the B. Interpret information provided above. It may help to think about what the hypothesis was and the kind of information that this experiment is meant to convey. C. What can this experiment tell you about how HIF-1 or CD18 affect cell survival during stroke? -1

Ciccarelli: Psychology_5 (5th Edition)
Ciccarelli: Psychology_5 (5th Edition)
5th Edition
ISBN:9780134477961
Author:Saundra K. Ciccarelli, J. Noland White
Publisher:Saundra K. Ciccarelli, J. Noland White
Chapter1: The Science Of Psychology
Section: Chapter Questions
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QUESTION 4 The experiment below examines what happens at the promoter of the CD18 gene during hypoxia (stroke). HIF-1 is a transcription factor that is stabilized during hypoxia and can bind to the Hypoxia Response Element (HRE). The top panel (A) shows a site in the CD18 promoter that HIF-1 can theoretically bind to (based on sequence only). In B, the experiment was performed to see if HIF-1 or a control protein (Cntl) actually bind this site under different conditions. A. What type of experiment is this? --) CD18 Gene Promoter A -79 ccacttcctccaaggaggagctgagaggaacaggaagtgtcaggacttta cgacccgcgcctccagctgaggtttctagACGTGacccaggg B HIF-1a IP Hypoxia Cntl IP CAGACT +19 HIF-1 HIF-1 Core Site Ancillary Site Hypoxia Response Element (HRE) Normoxia 166 bp B. Interpret the experiment in light of the information provided above. It may help to think about what the hypothesis was and the kind of information that this experiment is meant to convey. C. What can this experiment tell you about how HIF-1 or CD18 affect cell survival during stroke? -ts Save Answer
Transcribed Image Text:QUESTION 4 The experiment below examines what happens at the promoter of the CD18 gene during hypoxia (stroke). HIF-1 is a transcription factor that is stabilized during hypoxia and can bind to the Hypoxia Response Element (HRE). The top panel (A) shows a site in the CD18 promoter that HIF-1 can theoretically bind to (based on sequence only). In B, the experiment was performed to see if HIF-1 or a control protein (Cntl) actually bind this site under different conditions. A. What type of experiment is this? --) CD18 Gene Promoter A -79 ccacttcctccaaggaggagctgagaggaacaggaagtgtcaggacttta cgacccgcgcctccagctgaggtttctagACGTGacccaggg B HIF-1a IP Hypoxia Cntl IP CAGACT +19 HIF-1 HIF-1 Core Site Ancillary Site Hypoxia Response Element (HRE) Normoxia 166 bp B. Interpret the experiment in light of the information provided above. It may help to think about what the hypothesis was and the kind of information that this experiment is meant to convey. C. What can this experiment tell you about how HIF-1 or CD18 affect cell survival during stroke? -ts Save Answer
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