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A. In which phase of cell cycle would cells contain radioactive DNA? Would cells at all phases of the cell cycle contain radioactive DNA immediately after the labeling procedure? Explain briefly why B. Why are there initially no mitotic cells that contain radioactive DNA? Explain briefly C. Can you estimate the length of the G2 phase for these cells from the graph? Explain briefly D. Estimate the length of the total cell cycle for these cells.

A. In which phase of cell cycle would cells contain radioactive DNA? Would cells at all phases of the cell cycle contain radioactive DNA immediately after the labeling procedure? Explain briefly why B. Why are there initially no mitotic cells that contain radioactive DNA? Explain briefly C. Can you estimate the length of the G2 phase for these cells from the graph? Explain briefly D. Estimate the length of the total cell cycle for these cells.

Human Anatomy & Physiology (11th Edition)
Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
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Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
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If dividing cells are grown in a culture medium containing radioactive thymidine, the thymidine will be covalently incorporated into the cell's DNA during replication. Consider an experiment in which asynchronous cells are radioactively labeled for only a short period of time (about 30 minutes). The radioactive thymidine medium is then replaced with medium containing unlabeled thymidine, and the cells are grown for some additional time. At different time points after replacement of the medium, cells are examined in a microscope. The percentage of cells in mitosis (which can be easily recognized because their chromosomes are condensed) that have radioactive DNA in their nuclei is then determined and plotted as a function of time after the labeling with radioactive thymidine.
Transcribed Image Text:If dividing cells are grown in a culture medium containing radioactive thymidine, the thymidine will be covalently incorporated into the cell's DNA during replication. Consider an experiment in which asynchronous cells are radioactively labeled for only a short period of time (about 30 minutes). The radioactive thymidine medium is then replaced with medium containing unlabeled thymidine, and the cells are grown for some additional time. At different time points after replacement of the medium, cells are examined in a microscope. The percentage of cells in mitosis (which can be easily recognized because their chromosomes are condensed) that have radioactive DNA in their nuclei is then determined and plotted as a function of time after the labeling with radioactive thymidine.
100 75 50 25 5 10 15 20 25 30 time (hours) A. In which phase of cell cycle would cells contain radioactive DNA? Would cells at all phases of the cell cycle contain radioactive DNA immediately after the labeling procedure? Explain briefly why B. Why are there initially no mitotic cells that contain radioactive DNA? Explain briefly C. Can you estimate the length of the G2 phase for these cells from the graph? Explain briefly D. Estimate the length of the total cell cycle for these cells. labeled mitotic cells (%)
Transcribed Image Text:100 75 50 25 5 10 15 20 25 30 time (hours) A. In which phase of cell cycle would cells contain radioactive DNA? Would cells at all phases of the cell cycle contain radioactive DNA immediately after the labeling procedure? Explain briefly why B. Why are there initially no mitotic cells that contain radioactive DNA? Explain briefly C. Can you estimate the length of the G2 phase for these cells from the graph? Explain briefly D. Estimate the length of the total cell cycle for these cells. labeled mitotic cells (%)
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