Home Work:• Suppose you perform a PCR that begins with one double-strand of thefollowing DNA template:+5' -СТАССТСCGGGTTGACTGСТАССТТССССGGATGCCCAAAAТТСТСGAG-3—:::::::::::::::::::::::: ::::+3'-GATGGACССССААСТGACGATGGAAGGGCCCТАССGGTTTTAAGAGCTC-5'+A. Draw one cycle of PCR reaction below the following diagram.B. Label the template DNA, the primers, and what is happening at each step.(1)températurecycle #1

PCR is polymerase chain reaction that amplifies given DNA molecule into many copies of specific DNA…

Answered: A. Draw one cycle of PCR reaction below…

Biochemistry

9th Edition

ISBN:9781319114671

Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Chapter1: Biochemistry: An Evolving Science

Section: Chapter Questions

Problem 1P

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Define PCR. Write down different types of PCR in detail with their applications. Give the answer broadly.
5. Watch the video: https://www.youtube.com/watch?v=ml0Fo9kaWqo&t=38s. Based on the information in this video and material covered in class answer the following questions: A. In a Sanger sequencing-based PCR reaction, how many primers are added? B. In a Sanger sequencing-based PCR reaction, which molecules are more common: DNTPS or fluorescent terminators? C. What is a typical length of sequence that can be acquired using traditional (Sanger) sequencing? D. The results from sequencing one stretch of DNA is called a "read". How many reads can be obtained from a run on a 384-well plate Sanger sequencing instrument vs. a NovaSeg instrument? E. How many gjgabases of sequences can be obtained on the Sanger vs. NovaSea instruments? Regarding F. and G., the adaptor sequences have two parts: primer binding sites and capture sequences. F. Once the (denatured) DNA has been loaded into the flow cell, which adaptor sequence part is hybridized to other single-stranded DNA first? G. How many copies…
Discuss how the technique of PCR allows a scientist to quickly clone a particular piece of DNA.Be clear about the importance of 2 primers, Taq DNA polymerase, temp, and time. Explain howyou do a PCR – what is needed
Write about the PCR machine in detail
a. Describe the ingredients of a PCR reaction. b. List the different temperatures used in each cycle and tell why each is used.
Which of the following statements about PCR or RT-gPCR is correct? 1. Fluorescence is detected when the TagMan probe is cleaved by Taq polymerase (AMPLITAQ). 2. PCR uses the natural process of transcription to amplify DNA. 3. Both PCR and RT-qPCR require forward and reverse primers. 4. Fluorescence is detected when the reporter dye is in close proximity to the quencher dye. A. 1, 2 and 3 B. 1 and 3 C. 2 and 4 D. 4 only E. All of 1, 2, 3 and 4 are correct
Describe what PCR does. Name and explain its threesteps and give at least two uses for the PCR technique.
profile-image If you take a DNA photolyase- strain of E. coli, and subject it to the treatment below step 1, spread evenly on LB plate step 2, subject to UV light step 3, cover half of plate and grow in light What will be the most likely outcome if the level of UV is nearly lethal to cells on the plate? a. Only colonies on uncovered side of plate b. Only colonies on covered side of plate c. very few colonies in roughly equal numbers on both sides of plate
After four cycles of PCR, which type of PCR product predominates? Explain why.
Use the plasmid map for pBashi below to answer the following questions Xhol Hind!!! 15 kb 11 kb 10 kb 8 kb 7 kb 5 kb a. How large is pBashi? b. On the gel below, draw the bands corresponding to the expected pBashi cleavage products after restriction digest with the indicated restriction enzymes (Lane 2 - Xhol only, Lane 3 - HindIII only, Lane 4 - Xhol+ HindIII) 4 kb 3 kb 3 kb 2 kb 1 kb 4 kb Hindill 1 kb 7 kb Xhol Xhol Xhol Xhol Hind!!! Ladder Xhol Hindill Hind!!! Pstl Pstl c. Lane 5 in the gel above displays the pBashi cleavage products after restriction digest with the restriction enzymes Xhol+ Pstl. Lane 6 in the gel above displays the pBashi cleavage products after restriction digest with the restriction enzymes Xhol+ HindIII + Pstl. Indicate your final answers to the questions below clearly on the pBashi plasmid above; you will NOT receive any credit if you only list sizes of fragments below. If you need to, draft your answer somewhere else before writing your final answer above.…

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A. Draw one cycle of PCR reaction below the following diagram. B. Label the template DNA, the primers, and what is happening at each step. température cycle #1