Question 3: Look carefully at the structures of the two molecules shown below and answer thefollowing questions.O=ddCTPO=OHO11HO-P-O-P-O-P-OIOH OHNH₂ddCMPO11HO-P-OOHNH₂A) What would you expect to happen if you added ddCTP to a DNA synthesis reaction in vitroin large excess over the concentration of dCTP?B) What would happen if ddCTP were added at 10% of the concentration of dCTP?C) What effect would you expect if ddCMP were added to the synthesis in large excess overdCTP? At 10% of the concentration of dCTP?

ddCTP is 2',3'-Dideoxycytidine-5'-triphosphate. It does not contain a 3’OH group in the deoxyribose…

Answered: A) What would you expect to happen if…

Biochemistry

9th Edition

ISBN:9781319114671

Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Chapter1: Biochemistry: An Evolving Science

Section: Chapter Questions

Problem 1P

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Besides self-annealing, which of the following pairs of enzyme-digested DNA could pair up together to create a recombinant molecule? a) AluI can pair with any of the others. b) HindIII and BamHI can pair. c) All can pair. d) None can. e) Only BamHI and Sau3AI can pair.
How do we know that DNA synthesis is discontinuous on one of the two template strands?
What is proteinase K and what role does it play in DNA extraction?
Find out whether the following are true or false. a)Klenow polymerase is E. coli DNA polymerase I which has no 5' → 3‘ exonuclease activity. b)The best way to increase the amount of the product in PCR amplification is to increase the cycle number. c)In a PCR, primers must be identical to their template strand in order for hybridization to occur. d)The accuracy of the amplification with Taq DNA polymerase is very high due to its high 3’→ 5’ exonuclease activity
In a process of production of a recombinant protein by E. coli cells, it was observed accumulation of acetate in the culture medium. In this situation, it can be said that: (a) certainly the process in question was being conducted in anaerobiosis (B).Acetate accumulation is advantageous for the process as the acetate formation reaction generates 1 molecule of ATP (c)Knowing that decreasing the temperature of the process causes a reduction in the rate of glycolysis, this could be a strategy to reduce the accumulation of acetate (d).the acetate formed can be re-assimilated by the cell if the glyoxylate pathway is activated at some point in the culture
For the experiment shown Fig. 6.30 and 6.31, what is the advantage to use a labeled incoming nucleotide (α-32P-UTP or α-32P-CTP), not labeled reagent 1 itself? Please draw a diagram to help explain your answer by contrasting the RNA polymerase labeled with α-32P-Reagent (Panel A) vs α-32P-UTP (Panel B).
Identify the most mistaken (wrong) choice: a) Transcription machinery and an enhancer can bind to the chromosome at the same time. b) Organic matters may interfere with heat treatment of bacterial growth control. ( c) Nitrocellulose can be used to filter out microorganisms from a liquid solution. d) Time to kill a bacterial culture is not proportional to the number of microbes in the culture
After several rounds of replication, if COVID19 RNA changes from 5’ GGGUACAUGGUAGCCCCCGUCGAG …. 3’ to 5’ GGGUACAUGGUAGCCCCCGUCGCCCCGUAG …. 3’ Which of the following describes the above event? a) an insertion mutation occurred b) a duplication mutation occurred c) a multi-points mutation occurred d) a point-series mutation occurred e) an inversion mutation occurred
Which statement regarding transitions and transversions is TRUE? a) An adenine to guanine change is a transversion b) Transversions are more commonly found in nature that are transitions c) A cytosine to guanine change is a transition d) Transversions cause a distortion in the DNA double helix
Which of the following is NOT a function of DNA polymerase I in E. coli?Question 1 options: A) 5' exonuclease B) polymerase C) helicase
The wrong nucleotide is occasionally incorporated during DNA replication and not immediately repaired by the proofreading function of DNA polymerase. Which of the following DNA repair systems relies upon methylated DNA to determine which base is correct and which needs to be repaired?a) CPD photolyase repair b) mismatch repairc) nucleotide-excision repair d) base-excision repaire) homology-directed repair
Chimeric DNA true are all except - a) Formed by linking DNA fragments of unrelated 2ood l v genome b) Sticky end producing restriction endonucleases favour formation of chimeric DNA c) They don't require DNA ligases d) The organism harbouring a chimeric DNA has features of themselves and the properties of the insert

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