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A) What would you expect to happen if you added ddCTP to a DNA synthesis reaction in vitro in large excess over the concentration of dCTP? B) What would happen if ddCTP were added at 10% of the concentration of dCTP? C) What effect would you expect if ddCMP were added to the synthesis in large excess over dCTP? At 10% of the concentration of dCTP?

A) What would you expect to happen if you added ddCTP to a DNA synthesis reaction in vitro in large excess over the concentration of dCTP? B) What would happen if ddCTP were added at 10% of the concentration of dCTP? C) What effect would you expect if ddCMP were added to the synthesis in large excess over dCTP? At 10% of the concentration of dCTP?

Biochemistry
Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
Problem 1P
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Question 3: Look carefully at the structures of the two molecules shown below and answer the following questions. O= ddCTP O= OH O 11 HO-P-O-P-O-P-O I OH OH NH₂ ddCMP O 11 HO-P-O OH NH₂ A) What would you expect to happen if you added ddCTP to a DNA synthesis reaction in vitro in large excess over the concentration of dCTP? B) What would happen if ddCTP were added at 10% of the concentration of dCTP? C) What effect would you expect if ddCMP were added to the synthesis in large excess over dCTP? At 10% of the concentration of dCTP?
Transcribed Image Text:Question 3: Look carefully at the structures of the two molecules shown below and answer the following questions. O= ddCTP O= OH O 11 HO-P-O-P-O-P-O I OH OH NH₂ ddCMP O 11 HO-P-O OH NH₂ A) What would you expect to happen if you added ddCTP to a DNA synthesis reaction in vitro in large excess over the concentration of dCTP? B) What would happen if ddCTP were added at 10% of the concentration of dCTP? C) What effect would you expect if ddCMP were added to the synthesis in large excess over dCTP? At 10% of the concentration of dCTP?
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