(a) What are the different methods of cell lysis? Discuss in detail. (b) In Streptomyces filtration from an erythromycin broth using a test filter, we find the following data: Filtration time, sec 5 10 20 0.040 Volume of filtrate, L 0.055 0.080 The filter leaf has a total area of 0.009m² and the filtrate has a viscosity of 1.1 cP. The pressure drop is 0.51m of mercury and the feed contains 0.015 kg dry cake per liter. Determine the specific cake resistance a and the medium resistance RM. 30 0.095

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