a To make a genomic library useful for sequencingan entire genome, why would you ordinarily fragment the genomic DNA by mechanical shearingforces like sonication rather than by cutting theDNA with a restriction enzyme?b. Suppose that you wanted to make a genomic library to determine the complete sequence of anewly discovered organism’s genome, but you didnot have a sonicator readily available. Explain howyou could nonetheless use two or more restrictionenzymes to make libraries whose clones could besequenced so that a computer could assemble thegenomic sequence.c. Suppose you only had a single restriction enzymeavailable, and you want to make a single genomiclibrary from which you could assemble the genomic sequence. How might you be able to achievethis goal? (Hint: See Problem 9.) To make this library, would it be preferable to use a restriction enzyme that recognizes a 4-base, 6-base, or 8-basesequence of DNA?
a To make a genomic library useful for sequencing
an entire genome, why would you ordinarily fragment the genomic DNA by mechanical shearing
forces like sonication rather than by cutting the
DNA with a restriction enzyme?
b. Suppose that you wanted to make a genomic library to determine the complete sequence of a
newly discovered organism’s genome, but you did
not have a sonicator readily available. Explain how
you could nonetheless use two or more restriction
enzymes to make libraries whose clones could be
sequenced so that a computer could assemble the
genomic sequence.
c. Suppose you only had a single restriction enzyme
available, and you want to make a single genomic
library from which you could assemble the genomic sequence. How might you be able to achieve
this goal? (Hint: See Problem 9.) To make this library, would it be preferable to use a restriction enzyme that recognizes a 4-base, 6-base, or 8-base
sequence of DNA?
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