Is it possible that two different restriction enzymes couldcut the human genome into exactly the same number offragments and with exactly the same distribution of fragment sizes, yet the ends produced by the two enzymescould not be joined together by DNA ligase? Explain

Q: A) If one of the primers has a point mutation distinct from the template what is the percentage of…

A: PCR or polymerase chain reaction is a technique which is used in amplification of DNA or produce…

Q: Assuming that you start with one genome copy and are trying to amplify a 2000 bp DNA fragment, how…

A: PCR (Polymerase Chain Reaction) is a laboratory technique for amplifying a specific DNA segment. The…

Q: Describe the nature of recognition sites for restriction enzymes and the nature of the ends of the…

A: Vast uses of endonuclease activity of restriction enzyme found in the biotechnology for gene…

Q: How would the results of this activity have been different if the DNA sequence you digested were…

A: Gel electrophoresis is a process in which DNA fragments are separated on the basis of their size.…

Q: Meselson and Stahl grew bacteria in medium containing heavy nitrogen. After many cell divisions, the…

A: From Meselson and Stahl's experiment we have known that DNA replication is semi-conservative.

Q: For a molecular cloning experiment using the bacterial strain E. coli K12 is used to insert the gene…

A: Recombinant DNA technology uses enzymes and a variety of laboratory procedures to modify and isolate…

Q: Why is ligase needed to make recombinant DNA? Whatwould be the immediate consequence in the cloning…

A: DNA replication is the process of copying the DNA of the cell during cell division. DNA ligase is an…

Q: Wild-type E. coli takes up and concentrates a certain redfood dye, making the colonies blood red.…

A: As it is given that Wild-type E. coli concentrate the certain red dye by taking up it and thus make…

Q: Researchers are manipulating the gene cxx2 for their experiments, and they have inserted a small…

A: As per the central dogma of biology, the flow of genetic information is from DNA -> RNA ->…

Q: HOW Restriction Enzymes Cutthe Genome at Specific Sites?

A: Restriction enzymes are involved in accomplishing various biotechnological processes. Mostly found…

Q: A newly discovered insect has a genomic DNA that does not contain any repetitive DNA or any intronic…

A:

Q: you wanted to design two primers to PCR amplify a gene from an organism that has 4 chromosomes, each…

A: In PCR that is, polymerase chain reaction two primers are needed  to hybridize with the sample DNA…

Q: There are three classes of restriction enzymes; Class I, Class II and Class II. Nevertheless, Class…

A: Restriction endonuclease are the enzyme which make a cut in genetic material that is DNA somewhere…

Q: In producing genetically engineered human insulin in bacteria, why is it important to use the same…

A: In the production of human insulin by bacterium, E.coli, the human insulin gene is introduced into…

Q: The recognition sequence for the restriction enzyme Sau3AIis GATC; in the recognition sequence…

A: Given; The recognition sequence for the restriction enzyme Sau3AI is GATC. The recognition sequence…

Q: enzymes ECORI and HINDIII are called "compatible" enzymes. DNA cut with these enzymes can be ligated…

A: Restriction Endonucleases Restriction endonucleases or restriction enzymes are the enzymes that can…

Q: Calculate the average sizes and numbers of DNAfragments produced by digesting human genomic DNAwith…

A: DNA is the genetic material of many eukaryotic and prokaryotic organisms. It is a double-stranded…

Q: Why do restriction endonucleases not hydrolyze DNA from the organism that produces it?

A: Endonucleases are the enzymes, which help to cleave the DNA from inside that is in between the…

Q: If you wanted to create recombinant DNA using this enzyme(3' T A 5'), would you have to cut both…

A: DNA recombination is a technology in which DNA from different sources is isolated and recombined to…

Q: The temperature at which the primers and target DNA hybridize may be changed to influence the…

A: Introduction Temperature changes have an impact on the response, which may be noticed at both high…

Q: When performing cloning experiments, it is not always necessary to treat sources of DNA with the…

A: Isoschizomers are enzymes that can share the same recognition site in restriction digestions.

Q: What is a restriction digest? What does it mean if you were given a precut DNA?

A: Restriction enzyme digestion takes advantage of naturally occurring enzymes that cleave DNA at…

Q: A newly discovered insect has a genomic DNA that does not contain any repetitive DNA or any intronic…

A: Biotechnology is the use of our understanding of biological processes to develop useful applications…

Q: A 2.0kb bacterial plasmid ‘BS1030’ is digested with the restriction endonuclease Sau3A; the plasmid…

A: Plasmids are small, circular, double stranded, and extrachromosomal bacterial DNA. i.e. it is…

Q: As you learned in this chapter,restriction enzymes are sophisticated“scissors” that geneticistsand…

A: Making many, exact copies of a specific segment of DNA is known as DNA cloning. The gene or other…

Q: A PCR reaction was performed to amplify the XULA4 gene, which is bp 524-6,480 on a plasmid that is…

A: A plasmid is a circular DNA. Restriction enzymes cut DNA at specific sites called the restriction…

Genetic Evolution

Sexual reproduction produces distinct population variations. Heredity determines the traits and qualities to be passed down to the progeny. Over time, this variation within-species is also affected by the process of human evolution. The study of how genetic variation leads to evolutionary changes is known as genetic evolution. If a trait helps an organism to survive and reproduce, it is more likely to be passed down the generations. It is rare that such traits result in a completely distinct set of traits in descendants than the ancestral population; this can result in the emergence of a new species.