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A student planned to utilize HPLC to separate her chemically synthesized product (1) from th reactants (2 and 3). Predict the elution sequence for compounds 1, 2 and 3 based on the above information. Justify youranswer.

A student planned to utilize HPLC to separate her chemically synthesized product (1) from th reactants (2 and 3). Predict the elution sequence for compounds 1, 2 and 3 based on the above information. Justify youranswer.

Chemistry
Chemistry
10th Edition
ISBN:9781305957404
Author:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste
Publisher:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste
Chapter1: Chemical Foundations
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Problem 1RQ: Define and explain the differences between the following terms. a. law and theory b. theory and...
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A student planned to utilize HPLC to separate her chemically synthesized product (1) from th reactants (2 and 3).

Predict the elution sequence for compounds 1, 2 and 3 based on the above information. Justify youranswer.

HOʻ HO `N' H2N 1 Column: 250 x 30 mm C18 10u 100Å Flow: 5 mL/min Oven temperature: 25 oC Mobile Phase: A = water, Gradient: at 0 min 20 %B at 5 min 20 %B at 15 min 100 %B at 20 min 20 %B B = 90% Acetonitrile/Water Injection volume: 5 uL %3D 2.
Transcribed Image Text:HOʻ HO `N' H2N 1 Column: 250 x 30 mm C18 10u 100Å Flow: 5 mL/min Oven temperature: 25 oC Mobile Phase: A = water, Gradient: at 0 min 20 %B at 5 min 20 %B at 15 min 100 %B at 20 min 20 %B B = 90% Acetonitrile/Water Injection volume: 5 uL %3D 2.
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