A stock solution of the protein bovine serum albumin (BSA) of 10 mg/mL is provided to you. Using this stock solution prepare 2.0 mL of protein standards (BSA solutions of known concentration is referred to as a standard solution) with concentrations of 10.0 mg/mL 8.0 mg/mL, 6.0 mg/mL, 5.0 mg/mL, 4.0 mg/mL, 2.0 mg/mL, 1.0 mg/mL and 0.0 mg/mL As pre-lab exercise calculate the dilutions required to prepare each standard solution from the stock of 10.0 mg/mL To do so, use the formula CIV₁ =C2V2, where C₁ is the concentration of the stock solution 10.0 mg/mL, Vi shouldbe determined, C2 is the concentration of the standard being prepared and V2 is the final volume of standard being prepared, in this experiment 2.0 mL Tabulate your dilution calculations.

A stock solution of the protein bovine serum albumin (BSA) of 10 mg/mL is provided to you. Using this stock solution prepare 2.0 mL of protein standards (BSA solutions of known concentration is referred to as a standard solution) with concentrations of 10.0 mg/mL 8.0 mg/mL, 6.0 mg/mL, 5.0 mg/mL, 4.0 mg/mL, 2.0 mg/mL, 1.0 mg/mL and 0.0 mg/mL As pre-lab exercise calculate the dilutions required to prepare each standard solution from the stock of 10.0 mg/mL To do so, use the formula CIV₁ =C2V2, where C₁ is the concentration of the stock solution 10.0 mg/mL, Vi shouldbe determined, C2 is the concentration of the standard being prepared and V2 is the final volume of standard being prepared, in this experiment 2.0 mL Tabulate your dilution calculations.

Chemistry

9th Edition

ISBN:9781133611097

Author:Steven S. Zumdahl

Publisher:Steven S. Zumdahl

Chapter11: Properties Of Solutions

Section: Chapter Questions

Problem 108AE

See similar textbooks

Similar questions

A pharmacist prepares an isotonic saline solution for intravenous infusion. Instead of preparing a 0.15 M solution, a 1.5 M solution is prepared. What would happen to the red blood cells if this erroneously prepared solution is infused?
Fluoridation of city water supplies has been practiced in the United States for several decades. It is done by continuously adding sodium fluoride to water as it comes from a reservoir. Assume you live in a medium-sized city of 150,000 people and that 660 L (170 gal) of water is used per person per day. What mass of sodium fluoride (in kilograms) must be added to the water supply each year (365 days) to have the required fluoride concentration of 1 ppm (part per million)that is, 1 kilogram of fluoride per 1 million kilograms of water? (Sodium fluoride is 45.0% fluoride, and water has a density of 1.00 g/cm3.)
Explain the cleansing action of soap.
A compound has a solubility in water of 250 mg/L at 25C. Should this compound be characterized as a soluble or insoluble compound at 25C?
A patient has a “cholesterol count” of 214. Like manyblood-chemistry measurements,this result is measured inunits of milligrams per deciliter (mgdL1). Determine the molar concentration of cholesterol inthis patient’s blood, taking the molar mass of cholesterolto be 386.64gmol1. Estimate the molality of cholesterol in the patient’sblood. If 214 is a typical cholesterol reading among men inthe United States, determine the volume of such bloodrequired to furnish 8.10 g of cholesterol.
In the preparation of a standard stock solution for protein analysis, 50 mg of bovine serum albumin (BSA) was dissolved in deionized distilled water to a final volume of 10 mL. What is the weight of BSA in 0.60 mL stock solution?
MCC powder contains 86.6% proteins and WPI powder contains 93.4% protein. Calculate the amount of each protein POWDER required to obtain a 100 ml of 2% (w/v) protein solution.
14. The concentration of the original unknown protein solution is determined to be 0.2173 mg/mL. What is the protein concentration in the units of parts-per-million (ppm)? One ppm = 1 mg/L. 0.217 4602 217.3 217
How many kilograms (kg) of 20-20-20 soluble fertilizers should you add to a 50 gallon concentrated stock tank using and injector dilution ratio of 200:1 to provide a fertilizing solution of 200 mg N/L for plants plants?
First, a stock solution of 10 mM fluorescein must be made in 15 mL of water. Calculate the mass of fluorescein you need to weigh out to make this solution. Then, a dilution to 0.5 mM should be made in 15 mL of water. Calculate the volume of stock 10 mM fluorescein you need to add to make this solution. • Then, the following dilutions should also be made in 10 mL each (using the 0.5 mM stock): • Fluorescein: 0.0005, 0.001, 0.0025, 0.005, 0.01, 0.05, and 0.1 mM. Calculate the volume of fluorescein stock and water needed to make these dilutions.
The blood serum acetone for a person is determined to be 11.1 mg of acetone per 100 mL of serum. Express this concentration %v/v if the density for acetone liquid is 0.79 mg/mL.
1. A stock solution of I2 is standardized using a 0.1430 g sample of pure ascorbic acid. The titration to the starch endpoint required 25.45 mL of the I2 solution. Calculate the molarity of the I2 solution. 2. A vitamin C tablet was divided into three equal mass portions. One portion was crushed into a fine powder, dissolved in water, and titrated with the standardized solution from question 2 (above). The titration to the endpoint required 27.60 mL of the I2 solution. Calculate the number of milligrams of vitamin C that are in the original tablet.