A sample (660 mg) of a (homo)oligomeric protein of molecular weight of 132,000 Da was treated with an excess of 2,4-dinitro-fluorobenzene until the reaction was complete. The peptide bonds were then completely hydrolyzed by heating with 6 M hydrochloric acid. The hydrolysate was found to contain 5.5 mg of the following compound. NO2 O2N- -NH НООС 2,4-Dinitrophenyl derivatives of the amino groups of other amino acids were not found. a). How many polypeptide chains are there in this protein? Show your work. b). Assuming that each of the subunit contains entirely a-helices, how many a-helical turns would there be, approximately, in each of the subunit?

Q: amino acid in the form of a "phenylthiohydantoin" (PTH-amino acid), are shown below. What is the…

A: Phenyl isothiocynate is the Edman reagent. It reacts with N-terminal amino acid to give phenyl…

Q: Using the tree structures for the following monosaccharides and comparing to that for glucose, draw…

A: Carbohydrates are polyhydroxy aldehydes or ketones. They can be classified as monosaccharides,…

Q: A nonapeptide was determined to have the following amino acid composition: (Lys)2, (Gly) 2, (Phe) 2,…

A: Trypsin - Cleaves at Carboxyl terminus of basic amino acid such as lysine and arginine. Ahead of…

Q: Consider the structure of the tripeptide (in its fully protonated form) below. H O H I | || + O=C…

A: The amino acids contain ionizable groups. The ionizable groups are of the following types: the amino…

Q: Write down the values in bold print. You are isolating a protein with a pl of 8.5 using cation…

A: As given, protein of interest has pI of 8.5 which was isolated using cation exchange chromatography.…

Q: 6 Concanavalin (ConA) is a 25.5KDa protein with pI of 4.7 and optical absorbance (A 0.1% 289) of…

A: Proteins are polymers of amino acids with specific molecular weight and pI (isoelectric point, pH at…

Q: Determine the net charge (to the nearest integer) on the following peptide at pH5 AND pH 12.…

A:

Q: The 3 proteins have the following properties. Which is the correct order of steps to extract them?…

A: Protein extraction is a molecular biological method to isolate a particular one or two proteins…

Q: Calculate the isoelectric point of the tetrapeptide Ser-Leu-Phe-Pro at pH 7.0

A: Amino acid sequences are written with N-terminal amino acid on the left and C-terminal amino acid on…

Q: When answering the questions below, please use the one-letter code for the amino acid, with NO…

A: Acid hydrolysis: Acid hydrolysis of any protein/polypeptide is being used to detect amino acids…

Q: When separated on a polyacrylamide gel, the procedure is abbreviated as SDS-PAGE (Sodium Dodecyl…

A: SDS-PAGE is a biochemical technique, which is used to separate the proteins strictly on the basis…

Q: The reversible isomerization of (2-trans,6-trans)-farnesol into (2-cis,6-trans)-farnesol is…

A: Because, farnesol 2-isomer is a catalyst that will help in the conversion of…

Q: Ferritin is treated with SDS or SDS and b-mercaptoethanol and subjected to polyacrylamide gel…

A: SDS PAGE is used to separate proteins based on their size. SDS is an anionic detergent that imparts…

Q: The chemical structure of the tripeptide Met-Gin-Tyr is shown below. H₂N NH₂ Met-Gln-Tyr OH OH I

A: The attachment of covalent and non-covalent bonding of polyethylene glycol or PEG to various drugs…

Q: What is the difference of measuring proteins through UV-VIS spectroscopy at 280 nm and at 595 nm…

A: The proteins estimation is a routine process when working with different proteins. The protein…

Q: Using the generic structure shown, indicate what the substituents are for the structure of…

A: Substituents in the Finerenone-like Molecule:The chemical structure of a generic molecule. To…

Q: Spermaceti, a fragrant substance isolated from sperm whales, was commonly used in cosmetics until it…

A: Spermaceti can be described as a wax-like material present in sperm whales. It is present in liquid…

Q: Draw dipeptide Arg-Thr at pH 6.0. Circle the six atoms that are restricted in one plane. Calculate…

A: Dipeptide is simply 2 amino acids bonded to each other via a peptide bond. The two amino acids that…

Q: The structure of chitosan, a polymer used to eliminate protein clumps that create wine haze in white…

A:

Q: Write the one letter code for the oligopeptide Ser-Cys-Ile-Glu-Asn-Cys-Glu and calculate the total…

A: On a logarithmic scale from 0 to 14, pH, or "potential of hydrogen," measures the acidity or…

Q: Specific rotation of L-alanine [(+)alanine) in water (at 25 °C) is +2.8.  Suppose you have 2.00 g of…

A:

Q: Complete the following table by providing the Fischer and Haworth Projections of the given sugars.…

A: Fischer projection is a three-dimensional representation of sugar molecules in an open-chain form.…

Q: Explain how the (±)-lactic acid could be separated into its pure enantiomers by using (R)-1-…

A: Optical activity is the ability of the molecule to rotate the plane of polarized light because of…

Q: Suppose a mixture that has 10g of (-)-dextrose (a=-66) and 5g of (+)-dextrose (a=+66). Find the…

A: Rotation of a mixture: When polarized light is passed through any compound they have a uniques…

Q: Draw the tripeptide gly-glu-phe with all ionizable groups in their predominant state at pH 7.0.

A: A peptide is a short chain of amino acid residues linked together by a peptide bond. The peptide…

Q: Following are Fischer projections for a groupof five-carbon sugars, all of which are aldopentoses.…

A: Enantiomers are the pair of compounds, which are the mirror image but cant is superimposed on each…

Q: Given the following information about amino acid tyrosine answer questions 1 & 2: A. HO pka3 10.1…

A: Since you have posted multiple questions with multiple sub parts, we will provide the solution only…

Q: Hydrolysis of dodecapeptide P with the enzyme trypsin affords the following fragments: Arg,…

A: Trypsin and chymotrypsin are the proteolytic enzymes that can cleave peptide bonds of proteins with…

Q: A pentapeptide on complete hydrolysis yields 3 moles of glycine (R=H), 1 mole of alanine (R=CH3) and…

A: Proteins are the macromolecules composed of amino acids bound together by peptide bond between amino…

Q: Linear homoglycan of glucose connected by α1-4 linkages…

A: Polyssacharides are the long chains of carbohydrate molecules which are composed of several units of…

Q: 2. 60ml of a 0.2M solution of tetrapeptide Cys-Met-Arg-Asp is adjusted to pH=8. The solution is then…

A: Peptides are composed of acidic and basic groups with varying pKa values . Hence we introduce acids…

Q: Ornithine is an amino acid that is not used in the synthesis of proteins, but is an important…

A: Ornithine is an example of amino acid that does not take part in the process of protein formation…

Q: Serine is an uncharged, polar amino acid (below and left). Threonine (below and right) is another…

A: Amino acids are the building blocks of proteins which are composed of amino group (NH3+), carboxyl…

Q: The polymer chains of glycosaminoglycans are widelyspread apart and bind large amounts of water.a.…

A: The linear polymers that consist of repeating units of disaccharides are called glycosaminoglycan’s…

Q: Draw the keto or enol tautomer for each of the following compounds when they were treated with…

A: 1. Propanol on being treated with traces of acids or bases, its structure is converted to Propanone.…

Q: 80mL of a 0.3M solution of hexapeptide Leu-His-Cys-Glu-Asn-Arg is adjusted to pH=pI. The solution is…

A: Proteins are unbranched polymers constructed from 20 standard α-amino acids. They have four levels…

Q: Glutathione, a powerful antioxidant that destroys harmful oxidizing agents in cells, is composed of…

A: The substance which is produced by the liver naturally is called glutathione. It is present in meat,…

Nucleotides

It is an organic molecule made up of three basic components- a nitrogenous base, phosphate,and pentose sugar. The nucleotides are important for metabolic reactions andthe formation of DNA (deoxyribonucleic acid) and RNA (ribonucleic acid). 

Nucleic Acids

Nucleic acids are essential biomolecules present in prokaryotic and eukaryotic cells and viruses. They carry the genetic information for the synthesis of proteins and cellular replication. The nucleic acids are of two types: deoxyribonucleic acid (DNA) and ribonucleic acid (RNA). The structure of all proteins and ultimately every biomolecule and cellular component is a product of information encoded in the sequence of nucleic acids. Parts of a DNA molecule containing the information needed to synthesize a protein or an RNA are genes. Nucleic acids can store and transmit genetic information from one generation to the next, fundamental to any life form.