A sample (660 mg) of a (homo)oligomeric protein of molecular weight of 132,000 Da was treated with an excess of 2,4-dinitro-fluorobenzene until the reaction was complete. The peptide bonds were then completely hydrolyzed by heating with 6 M hydrochloric acid. The hydrolysate was found to contain 5.5 mg of the following compound. NO2 O2N- -NH НООС 2,4-Dinitrophenyl derivatives of the amino groups of other amino acids were not found. a). How many polypeptide chains are there in this protein? Show your work. b). Assuming that each of the subunit contains entirely a-helices, how many a-helical turns would there be, approximately, in each of the subunit?
Nucleotides
It is an organic molecule made up of three basic components- a nitrogenous base, phosphate,and pentose sugar. The nucleotides are important for metabolic reactions andthe formation of DNA (deoxyribonucleic acid) and RNA (ribonucleic acid).
Nucleic Acids
Nucleic acids are essential biomolecules present in prokaryotic and eukaryotic cells and viruses. They carry the genetic information for the synthesis of proteins and cellular replication. The nucleic acids are of two types: deoxyribonucleic acid (DNA) and ribonucleic acid (RNA). The structure of all proteins and ultimately every biomolecule and cellular component is a product of information encoded in the sequence of nucleic acids. Parts of a DNA molecule containing the information needed to synthesize a protein or an RNA are genes. Nucleic acids can store and transmit genetic information from one generation to the next, fundamental to any life form.

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