A researcher is examining the distribution of an ion channel protein in the kidney. She incubates slices of kidney tissue in a dilute solution of a specific antibody directed against the protein, then uses the immunoperoxidase method to localize the ion channel proteins. She notes the presence of brown pigment in a population of epithelial cells, which on closer examination, have a brush border. The researcher concludes that the protein is probably present in cells of the A. collecting duct B. deep portion of loop of Henle C. distal convoluted tubule D. glomerulus E. proximal convoluted tubule

A researcher is examining the distribution of an ion channel protein in the kidney. She incubates slices of kidney tissue in a dilute solution of a specific antibody directed against the protein, then uses the immunoperoxidase method to localize the ion channel proteins. She notes the presence of brown pigment in a population of epithelial cells, which on closer examination, have a brush border. The researcher concludes that the protein is probably present in cells of the A. collecting duct B. deep portion of loop of Henle C. distal convoluted tubule D. glomerulus E. proximal convoluted tubule

Human Anatomy & Physiology (11th Edition)

11th Edition

ISBN:9780134580999

Author:Elaine N. Marieb, Katja N. Hoehn

Publisher:Elaine N. Marieb, Katja N. Hoehn

Chapter1: The Human Body: An Orientation

Section: Chapter Questions

Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...

See similar textbooks

Similar questions

Write the introduction of antibodies in biotechnology and write a short history of antibodies? Please answer at your own words.
Our Western Blot experiment requires a buffer called TBS. At working concentration (1X), this buffer contains: 20 mM Tris 150 mM NaC1. Prior to use, however, the buffer is made and stored as a 10X concentrated stock What are the concentrations of these two ingredients in the 10X stock? Primary antibodies for the Western Blot experiment are prepared using this TBS buffer, with the addition of several other ingredients. Calculate the amount of each ingredient needed to produce10 ml of the final working antibody solution. b. 10X TBS stock c 0.01% (v/v) Tween detergent d. 3% (w/v) BSA (molecular weight 66430 g/mol) e. 1:10,000 dilution of antibody stock
Construct a typical antibody (immunoglobulin) molecule. Label the variable and constant regions, and the light and heavy chains. Indicate which region determines the antibody’s isotype (class). Indicate which region determines its antigenic specificity.
1) SDS PAGE gels can be probed with NBT/BCIP to perform an immunoblot true/ false 2) the direction of migration of protiens through a matrix is due to most proteins in natur having a net negative charge true/false 3) PBST is a buffer that is used for blocking the membrane during immunoblotting. true/false 4) the buffer used during for transferring proteins to nitrocellulose methanol. true/false 7) The size of the pores of a polyacrylamide gel dcreases as concentrations of polyacrylamide increases. True or false 10) The secondary antibody used in laboratory for western blotting is conjugated to which enzyme A) Alkaline decarboxylase B) GAPDH C) Alkaline Phosphatase D) Horseeradish peroxide 13) In protien electrophroresis, what reagent present in the sample buffer is used to eliminate difference in the charge densities of protein A) SDS B) 2- mercaptoethanol C) Tris-HCL pH6.8 D) Tris- HCL PH 8.8
You extract B cells and dilute the cells so there’s one B cell in each well of a 10-well plate. You then add nothing to 1 of the wells (negative control). To the remaining 9 wells, you add various intact bacterial cells to the wells (a few cells of 1 strain of bacteria per well). After a day, you remove the contents of each of the 10 wells, and examine each of them under a microscope. For the control, you find the one B cell you started with. For 8 of the 9 samples when bacteria were added, you find the one B cell you started with. But for 1 of the 9 samples when bacteria were added, you find 100s of B cells. What does this tell you about the original B cell from the well where you now see 100s of B cells?
During IHC staining, where would alpha-tubulin show in the esophagus image below (see image)?
Which components are considered a barrier to pathogen entry? all that apply Select one or more: a. PRRs b. plasma cells c. mucus d. lymph nodes e. lysozyme
Why does the eliza with two antibodies incubate after adding the substrates?
The function of the integrin can be described as follows:a). Media signal translation insideb). Stabilizes the binding of the TCR so that receivers are added and signals are sent insidec). Allows the receiver and its corrector to stick to the antigend). It helps the movement and development of lipid rafts that add and concentrate antigens together with TCR
Addition of immunoglobulin G (IgG) specific for hemoglobin to a solution of hemoglobin results in the formation of a red precipitate. In contrast, addition of the Fab fragments from this antibody to hemoglobin results in no such precipitate. What could explain this difference in results? Treatment with papain produces Fab fragments with different antigen specificity than the original IgG molecule. IgG can simultaneously bind two different antigens, whereas an Fab fragment can only bind one antigen at a time. The Fab fragments preferentially bind to other Fab fragments rather than to hemoglobin. The hemoglobin molecule antibody-binding sites can bind IgG molecules, but cannot bind Fab fragments.
if our immume systems are capable of attacking the trypanosome, why do you think we might see the number of parsites in a untreated person's blood repeatedly rebound in that way?
choose correct option and do explain.