a) Predict whether fumarate or maleate will have greater electrophoretic mobility. Cis-smaller than trans CO2 CO2 fumarate maleate b) Electrophoresis is run with the injection end positive and the detection end negative with a buffer with pH = 8.5. The charge on both ions is -2 at pH = 8.5. The electroosmotic flow is greater than the electrophoretic flow. Predict the order of elution of these two ions. At pH = 4.0, both anions have a charge of -1. At this pH, the injection end is negative, and the detection end is positive. Why is the polarity switched at lower pH?
a) Predict whether fumarate or maleate will have greater electrophoretic mobility. Cis-smaller than trans CO2 CO2 fumarate maleate b) Electrophoresis is run with the injection end positive and the detection end negative with a buffer with pH = 8.5. The charge on both ions is -2 at pH = 8.5. The electroosmotic flow is greater than the electrophoretic flow. Predict the order of elution of these two ions. At pH = 4.0, both anions have a charge of -1. At this pH, the injection end is negative, and the detection end is positive. Why is the polarity switched at lower pH?
Chemistry
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ISBN:9781305957404
Author:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste
Publisher:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste
Chapter1: Chemical Foundations
Section: Chapter Questions
Problem 1RQ: Define and explain the differences between the following terms. a. law and theory b. theory and...
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Transcribed Image Text:4. a) Predict whether fumarate or maleate will have greater electrophoretic mobility. Cis-smaller than trans
.CO2
"O2C
CO2
fumarate
maleate
b) Electrophoresis is run with the injection end positive and the detection end negative with a buffer
with pH = 8.5. The charge on both ions is -2 at pH = 8.5.
The electroosmotic flow is greater than the electrophoretic flow. Predict the order of elution of these
two ions. At pH = 4.0, both anions have a charge of -1.
At this pH, the injection end is negative, and the detection end is positive. Why is the polarity switched
at lower pH?
Predict the order of elution of the two ions.
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