A conditional mutation when there is an alteration to the coding region of a gene is caused by a. Base addition. Choose below. X-ray exposure Gene deletion Vaping Tautomeric shift
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A conditional mutation when there is an alteration to the coding region of a gene is caused by a. Base addition. Choose below.
- X-ray exposure
- Gene deletion
- Vaping
- Tautomeric shift
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Solved in 2 steps
- Give the SIGNIFICANT difference between the terms provided in the table below. Somatic mutation Germline mutation Turner’s Syndrome Down Syndrome Muscular Dystrophy Burkitt’s Lymphoma Deletion Duplication Translocation Inversion Transition Transversion Missense nonsense Point Mutation Frameshift Mutation Intercalating Agents Alkylating Agents Based Excision Repair Mismatch RepairGive the SIGNIFICANT difference between the terms provided in the table below. NO NEED for complete sentences, phrases will do. Somatic mutation Germline mutation Turner’s Syndrome Down Syndrome Muscular Dystrophy Burkitt’s Lymphoma Deletion Duplication Translocation Inversion Transition Transversion Missense nonsense Point Mutation Frameshift Mutation Intercalating Agents Alkylating Agents Based Excision Repair Mismatch RepairThe right-hand column contains descriptions of 5 types of molecular markers. Label each description with the name of the being described. Single-nucleotide polymorphism (SNP) Sequence-tagged site (STS) Microsatellite Amplified restriction fragment length polymorphism (AFLP) Restriction fragment length polymorphism (RFLP) Reset Zoom TABLE 23.1 Common Types of Molecular Markers Marker Description A site in a genome where the distance between two restriction sites varies among different individuals. These sites are identified by restriction enzyme digestion of chromosomal DNA and the use of Southern blotting. Amplified restriction fragment length polymorphism (AFLP) The same as an RFLP except that the site is amplified via PCR instead of isolating the chromosomal DNA. A site in the genome that contains many short sequences that are repeated many times in a row. The total length is usually in the range of 50-200 bp, and the length of a given microsatellite may be polymorphic within a…
- Fill the Table with mutagenic agents and provide their type (physical, chemical, biological) and their classification based on their effect (teratogenic, carcinogenic, clastogenic, or non-specific), together with their modes of action. Mutagen Type of Mutagen Classification based on effect Action UV – radiation X-Ray radiation Virus Heat 5-bromouracil Ethidium bromide 2-aminopurine Acridine orange Proflavine Cobalt Nickel Methylhydrazine Temozolomide Ethyl ethane sulfateTitled “Techniques utilized to generate a genetically engineered organism and confirm gene disruption.” a. In the table, indicate with an "x" if the reagent or technique applies to DNA, RNA, and/or protein. You can have more than one "x" in each row or none at all.You have a patient in your clinic presenting symptoms of cystic fibrosis. You screen their CFTR gene for mutations, and find the following list: CFTR 320 L V CFTR 341 S W CFTR 528 E D CFTR 976 F Q CFTR 1235 S R Which mutation(s) are likely causing cystic fibrosis in this patient? You also sequence a newborn family member of this patient. They have all of these same mutations, other than the one at position 976, and no other mutations in CFTR. Do you predict this person will develop cystic fibrosis? Explain why.
- Define these types of mutation: Inversion Insertion Translocation Deletion DuplicationSTR markers: are point mutations detectable by DNA sequencing are variations in the number of repeats of very short DNA motifs (2-10 nucleotides) □have high polymorphism are mutations leading to proteins or blood groups that can be differentiated by antigenic testing from a blood sample ☐have low polymorphism no correct answer are changes of a few nucleotides leading to the absence or presence of a site recognized by a restriction enzyme are variations in the number of repeats of medium-sized DNA motifs (10-100 nucleotides) can be located in coding sequences are located exclusively on autosomesa. Using nucleotide letters, show the kind of cut that could be made on a DNA molecule to circularize it into a plasmid. b. What are restriction length polymorphisms, and how are they used?
- The best molecular technique to quantify the gene transcripts is (write in full).The human gene for hemophilia is on the X chromosome. Below is a pedigree from a family afflicted with hemophilia. The man with blackened symbol (III-1) has hemophilia. To help with genetic diagnosis, a probe that detects an RFLP (restriction fragment length polymorphism) on the X chromosome is used. This probe detects either a 7 kb restriction enzyme fragment or 3 kb and 4 kb restriction enzyme fragments. The RFLP pattern for all the members of the pedigree is shown. The recombination distance between the RFLP and the hemophilia locus is 40%. What is the chance that their unborn child (III-7) is affected? 4 kb 3 kb Select one: a. 10% b. 20% C. 40% d. 60% e. 80% f. 90% g. None of these above II IIAfter restriction enzymes cut, they contain unpaired bases. Type II restriction enzymes leave ends that may be 5' overhanging, 3' overhanging, or blunt. In all cases each end is left with a 3' OH and a 5' phosphate. All blunt ends, and any complementary overhanging ends may be re-ligated with T4 DNA ligase, as long as at least one 5'- phosphate is present. In the tables below G^AATTC means that the end after cutting with enzyme will be: -----G 3' -----CTTAA 5' GTGCA^C means that the end will be: -----GTGCA 3' -----C 5' Which RE’s from table below have a 5’ overhang? Which ones have a 3’ Overhang? AccI GT^CGAC BamHI G^GATCC ClaI AT^CGAT NsiI ATGCA^T PstI CTGCA^G BglII A^GATCT TaqI T^CGA