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A blood plasma sample is electrophoresed. The sample is applied in the middle of the gel (i.e. the 'origin' is halfway down on the gel) and the gel is stained with a lipoprotein-specific stain. The pl yalues of the lipoproteins in this sample are shown below:

A blood plasma sample is electrophoresed. The sample is applied in the middle of the gel (i.e. the 'origin' is halfway down on the gel) and the gel is stained with a lipoprotein-specific stain. The pl yalues of the lipoproteins in this sample are shown below:

Biochemistry
Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
Problem 1P
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QUESTION 8 A blood plasma sample is electrophoresed. The sample is applied in the middle of the gel (i.e. the 'origin' is halfway down on the gel) and the gel is stained with a lipoprotein-specific stain. The pl values of the lipoproteins in this sample are shown below: Chylomicron - 8.6 LDL - 6.8 VLDL - 6.5 HDL - 5.2 To answer the question below, you make the assumption that migration is solely dependent on charge and that the distance migrated is linearly related to charge. When only taking charge into account, which ONE of the following statements does NOT complete the sentence below correctly? During gel electrophoresis at pH 8.2 the: During gel electrophoresis at pH 8.2 the: chylomicrons migrate the shortest distance from the origin and are the only lipoprotein particle a that is positively charged. lipoproteins belonging to the endogenous pathways are negatively charged and move towards o the positively charged anode. distance that the LDL particles migrate from the origin is 2.5-fold greater than the distance the o chylomicrons migrate from the origin. HDL, VLDL and LDL particles are all negatively charged but LDL migrates closest to the positively o charged anode.
Transcribed Image Text:QUESTION 8 A blood plasma sample is electrophoresed. The sample is applied in the middle of the gel (i.e. the 'origin' is halfway down on the gel) and the gel is stained with a lipoprotein-specific stain. The pl values of the lipoproteins in this sample are shown below: Chylomicron - 8.6 LDL - 6.8 VLDL - 6.5 HDL - 5.2 To answer the question below, you make the assumption that migration is solely dependent on charge and that the distance migrated is linearly related to charge. When only taking charge into account, which ONE of the following statements does NOT complete the sentence below correctly? During gel electrophoresis at pH 8.2 the: During gel electrophoresis at pH 8.2 the: chylomicrons migrate the shortest distance from the origin and are the only lipoprotein particle a that is positively charged. lipoproteins belonging to the endogenous pathways are negatively charged and move towards o the positively charged anode. distance that the LDL particles migrate from the origin is 2.5-fold greater than the distance the o chylomicrons migrate from the origin. HDL, VLDL and LDL particles are all negatively charged but LDL migrates closest to the positively o charged anode.
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