a) 500ml of 2M Tris-HCl buffer pH 8.9. Tris base MW is 121.14 g/mol. You will use concentrated HCI to adjust the pH. Describe the preparation procedure for this buffer and list the materials needed.

Biochemistry
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Chapter1: Biochemistry: An Evolving Science
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Please solve for A and please show all the work calculations
Address the questions regarding the preparation of the following solutions. Show all
of your calculations.
a) 500ml of 2M Tris-HCl buffer pH 8.9. Tris base MW is 121.14 g/mol. You will
use concentrated HCI to adjust the pH. Describe the preparation procedure for
this buffer and list the materials needed.
b) 10ml of 20% (w/v) L-arabinose stock (weight/volume). How much L-arabinose
do you need to prepare desired arabinose stock?
c) 100ml of LB media with: 25 µg/ml of Amp; and 100 µg/ml of Kan
concentrations.
You have:
● 100ml of LB
Amp stock at 100 mg/ml
Kan stock at 50 mg/ml
-
final
Determine how much of each antibiotic stock solution you need to add to 100ml
of LB to reach desired antibiotic concentration.
d) 50 ml of a solution containing each of the following: 10% Acrylamide, 0.05%
BIS, 0.1M Tris-HCI pH 8.9.
You have 0.5L of each of the following:
40% (w/v) Acrylamide
Transcribed Image Text:Address the questions regarding the preparation of the following solutions. Show all of your calculations. a) 500ml of 2M Tris-HCl buffer pH 8.9. Tris base MW is 121.14 g/mol. You will use concentrated HCI to adjust the pH. Describe the preparation procedure for this buffer and list the materials needed. b) 10ml of 20% (w/v) L-arabinose stock (weight/volume). How much L-arabinose do you need to prepare desired arabinose stock? c) 100ml of LB media with: 25 µg/ml of Amp; and 100 µg/ml of Kan concentrations. You have: ● 100ml of LB Amp stock at 100 mg/ml Kan stock at 50 mg/ml - final Determine how much of each antibiotic stock solution you need to add to 100ml of LB to reach desired antibiotic concentration. d) 50 ml of a solution containing each of the following: 10% Acrylamide, 0.05% BIS, 0.1M Tris-HCI pH 8.9. You have 0.5L of each of the following: 40% (w/v) Acrylamide
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