Please solve for A and please show all the work calculations Address the questions regarding the preparation of the following solutions. Show allof your calculations.a) 500ml of 2M Tris-HCl buffer pH 8.9. Tris base MW is 121.14 g/mol. You willuse concentrated HCI to adjust the pH. Describe the preparation procedure forthis buffer and list the materials needed.b) 10ml of 20% (w/v) L-arabinose stock (weight/volume). How much L-arabinosedo you need to prepare desired arabinose stock?c) 100ml of LB media with: 25 µg/ml of Amp; and 100 µg/ml of Kanconcentrations.You have:● 100ml of LBAmp stock at 100 mg/mlKan stock at 50 mg/ml-finalDetermine how much of each antibiotic stock solution you need to add to 100mlof LB to reach desired antibiotic concentration.d) 50 ml of a solution containing each of the following: 10% Acrylamide, 0.05%BIS, 0.1M Tris-HCI pH 8.9.You have 0.5L of each of the following:40% (w/v) Acrylamide

A buffer is a solution that can resist changes in pH when small quantities of acid or base is added…

Answered: a) 500ml of 2M Tris-HCl buffer pH 8.9.…

a) 500ml of 2M Tris-HCl buffer pH 8.9. Tris base MW is 121.14 g/mol. You will use concentrated HCI to adjust the pH. Describe the preparation procedure for this buffer and list the materials needed.

Biochemistry

9th Edition

ISBN:9781319114671

Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Chapter1: Biochemistry: An Evolving Science

Section: Chapter Questions

Problem 1P

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