A 500. µl aliquot of a caffeine unknown is diluted to a total volume of 10.00 mL. A calibration curve for caffeine was generated using external standards on the HPLC. The equation of the curve Caffeine Area(mV cm)=1.266C(ppm)+0.029. If the HPLC peak for the diluted unknown has an area of 3.07 mV cm, what is the original caffeine concentration in ppm?

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A 500. µl aliquot of a caffeine unknown is diluted to a total volume of 10.00 mL.
A calibration curve for caffeine was generated using external standards on the HPLC. The
equation of the curve
Caffeine Area(mV cm)=1.266C(ppm)+0.029.
If the HPLC peak for the diluted unknown has an area of 3.07 mV cm, whąt is the original
caffeine concentration in ppm?
Transcribed Image Text:A 500. µl aliquot of a caffeine unknown is diluted to a total volume of 10.00 mL. A calibration curve for caffeine was generated using external standards on the HPLC. The equation of the curve Caffeine Area(mV cm)=1.266C(ppm)+0.029. If the HPLC peak for the diluted unknown has an area of 3.07 mV cm, whąt is the original caffeine concentration in ppm?
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