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- 1. What is the purpose of the different reagents used in the procedure of kato-katz technique? 2. How are the Kato Katz Technique results reported? 3. What are the advantages and disadvantages of using Kato-Katz Technique?1. If excipients do not have pharmacodynamic activity, how do excipients affect the performance of the drug product? 2. What is meant by the rate-limiting step in drug bioavailability from a solid oral drug product? 3. What is the usual rate-limiting step for a poorly soluble and highly permeable drug (BCS 2)? 4. How could the manufacturing process affect drug product performance? 5. Drug absorption involves at least three distinct steps: dissolution, permeation, and disposition during transit in GI (an additional step of drug disposition in the body is involved as well for bioavailability). How are these processes validated in vitro when the in vivo requirement for drug bioavailability is waived? 6. What is meant by “sink” conditions? 7. What physical or chemical properties of a drug substance are important in designing a drug for (a) oral administration or (b) parenteral administration? 8. For a lipid-soluble drug that has very poor aqueous solubility, what strategies could be…2. The following data were generated after a set of bovine serum albumin standards were measured via the BCA assay. Table 1. Absorbance measurements at 562 nm for BSA standards measured in triplicate in a BCA Assay. Standards A B C D E F G H I Conc (ug/ml) 2000 1500 1000 750 500 250 125 25 0 Replicate 1 Abs @ 562 nm 2.381 2.039 1.505 1.143 0.819 0.454 0.245 0.056 0 Replicate 2 Abs @ 562 nm 2.456 2.052 1.489 1.156 0.823 0.46 0.272 0.049 0 Replicate 3 Abs @ 562 nm 2.43 2.05 1.47 1.17 0.81 0.48 0.255 0.053 0.00 Use Excel or your graphing program of choice to create a standard curve using the data from table 1. Consider the best way to graph this data. Make sure the type of graph that you select will allow you to add a line of best fit. Display the equation for the line of best fit directly on the graph. Hint... you'll want to think about how to treat the triplicate values when making the graph. Also consider the type of graph that should be selected to allow for addition of the line of…
- 4.Why is it important to include control groups and placebos in studies of the ergogenic properties of any substance or phenomenon?2a.) Illustrate below the image you see when you observe the onion skin with the 4x and 40x objectives. How does the addition of iodine change what you observe?1. What are the reasons why the products based on onion and garlic that use against COVID-19 can be placed in the market? Explain and discuss it thoroughly. I need a comprehensive answer.
- 6. assay kits that determine the amount of glucose present4. A physician orders an IV drip containing 12 million units per day of penicillin G potassium for a patient with otitis media (middle ear infection). A. If 18.2 mL of diluent is used to reconstitute the penicillin G, how many mL should be injected? B. If 8.2 mL of diluent is used to reconstitute the penicillin G, how many mL should be injected? C. If 3.2 mL of diluent is used to reconstitute the penicillin G, how many mL should be injected?Which of the following statements is/are FALSE about Bradford assay? 1. Upon addition of the reagent, the color of the protein solution becomes purple. II. The absorbance of the resulting solution is measured at a wavelength of 540-550 nm. Both I and II O II only Neither I nor II O I only