Q: Define the following terms: a. Chermolithoheterotroph b. Microaerophile c. Chermoorganoheterotroph…
A: The living world, as we all know it, can predominantly be divided into - Plants and Animals. Apart…
Q: The reason why most antibiotics as oral suspension dosage forms are commercially available as dry…
A: Antibiotics are the drugs used for the treatment of bacterial infection. Oral antibiotic are always…
Q: 1. An organism that has peroxidase and superoxide dismutase but lacks catalase is most likely an
A: Peroxidase - are enzyme which breaks up the peroxides (R-OO) into R-OH and R'-OH Superoxide…
Q: Explain why Griffith’s mixture of harmless dead bacteria possessing capsules and harmless live…
A: The laws of inheritance was discovered by Gregor Johann Mendel who is popularly known as the father…
Q: Why are biochemical tests used to identify microbes?
A: Microbes are living organisms that are very small and can be seen only with the help of a…
Q: Why is CFU more applicable to a culture of streptococcus than to a culture of E.coli
A: Streptococcus is a Gram-positive bacteria that are spherical in shape. This bacteria is responsible…
Q: 1. What is the purpose of the different reagents used in the procedure of kato-katz technique? 2.…
A: the Kato-Katz approach is used for qualitative and quantitative diagnosis of intestinal helminthic…
Q: What modifications can be made to microbial metabolicprocesses in order for them to produce…
A: The small microscopic and unicellular organisms are called microorganisms. There are many types of…
Q: Coulter Counter method in microbiology and its advantages and disadvantages?
A: Microbiology is the branch o biology that is concerned with the research of unicellular,…
Q: 1. Escherichia coli but not Pyrolobus fumarii will grow at 40°C, while P. fumarii but not E. coli…
A: Thank you for the question Answer :- Microbes are classified based on environment as mesophiles,…
Q: What are the enzyme assays for
A: Enzymes are biocatalyst that increases the speed of reaction by lowering the activation energy.…
Q: The catalase test determines the presence of the catalase enzyme. a. Describe the function of the…
A: 3. In microbiology, catalase test is a very important test to identify bacteria. This test helps to…
Q: List 3 advantages and 3 disadvantages of the protein assays Biuret, Folin (Lowry), and Bradford.
A: Protein assays used in determining the protein concentrations. Different protein assay techniques…
Q: Discuss the quality criteria for nucleic acid isolates. Explain the use of UV spectrometry in…
A: Nucleic acids are the macromolecules;which play important role in storing genetic information.DNA…
Q: Koch's postulates include all of the following EXCEPT O 1) Isolate the organism in pure culture. The…
A: Koch's postulates are a series of findings and experimental criteria suggested in the late 1800s by…
Q: 3 C Which gel in SDS-PAGE is used for samples separation?
A: Introduction A laboratory technique called gel electrophoresis is used to separate mixture of DNA,…
Q: What is known as montreal protocol?
A: Montreal protocol is an international initiative for ozone depletion.
Q: If you had cultures of E. coli and E. aerogenes and forgot to label them, which of the following…
A: E. aerogenes is indole-negative but E. coli is indole positive i.e., indole positive bacteria can…
Q: Toreterate, in your own words what is 1. The purpose of the selective factor in combination media? 2…
A: In microbiology, one of the most important components is the medium. Microorganisms such as…
Q: Laboratories spend millions of dollars and person-hours to identify microbes from clinical samples.…
A: Flagella are organelles that are characterised by their function rather than their appearance. The…
Q: 2. Leaching is known as a heterogenous process. Describe what types of leaching processes can be…
A: The leaching process is used to extract substances from solids. When a substance is allowed to…
Q: 3. Identify the IMVIC pattern of the following tubes. For set of tubes that is E. coli positive,…
A: The IMViC tests are a collection of separate assays used in microbiological labs to detect coliform…
Q: Match the following methods of Analysis Fractional analysis, methylation, and periodate oxidation A.…
A: Methods of analysis are different techniques used to analyze a compound to determine its structure…
Q: Briefly comment on the use of alkaline phosphatase assays as a diagnostic aid for a single named…
A: Alkaline phosphatases are enzymes that catalyze extracellular organic phosphate esters. These…
Q: Describe the different types of chromatography used to purify proteins.
A: Introduction: Protein purification is a collection of procedures for isolating one or a few proteins…
Q: Match the following methods of Analysis v Fractional analysis, methylation, and periodate oxidation…
A: The Biochemical analysis techniques are a set of methods, procedures and assays. This enables…
Q: 4. Suppose after an analysis of lake water used for drinking that you concluded that coliforms were…
A: Califorms are the group of bacteria that commonly lives in the intestine of animals and also found…
Q: Name 4 steps that you can use to isolate a protein from a liver homogenate. Also, briefly discuss…
A: Proteins can be isolated from bacteria or mammalian cells or tissue lysate. Protein extraction and…
Q: Outline the strategies commonly used to detect for pBR322 in E.coli
A: Plasmids are DNA molecules that exist outside of the chromosomes. They are microscopic, round, and…
Q: Phenol Red Test Question:
A: Phenol Red Broth is a differential test medium typically used to differentiate gram-negative enteric…
Q: Would detection of fecal coliforms (ex E. c/oli) in meat be indicative of contamination or spoilage…
A: Pathogenic bacteria are harmful to both people and the environment. Spoilage of a food item is…
Q: Why is it Koch's postulates cannot be used for the microbe E. Coli. What limitations does E.coli…
A: The virulence and pathogenicity is considered as the ability of bacteria to cause disease. Robert…
Q: Cite the qualitative tests to characterize the products of RNA hydrolysis. Indicate the reagents…
A: RNA is a nucleotide, so the product of hydrolysis will be its constituents molecule, which will help…
Q: o NB4: Why do you think you first had to make the 1 mM solution instead of working directly from the…
A: A working solution is different from Stock solution. A Working solution is prepared from stock…
Q: in the isolation of alkaloid,in the early stages of acid or base required conditions. explain the…
A: Alkaloids are secondary metabolites derived through shikimic acid pathway. They are nitrogen…
Q: State the principle that underlies the following biochemical tests: a) Methyl red test
A: Fermentation is an anaerobic process where glucose or other carbohydrates are metabolized to produce…
Q: ive two reasons why E. coli is naturally resistant to antibiotics like Penicillin?
A: Bacterial survival in an adverse situation, in a substrate is given as the ability of bacteria to…
Q: 1. Briefly discuss the folowing; (a) sources of microorganisms in low-heat-processed meat products;…
A: For the creation of food and food ingredients, such as wine, beer, bakery goods, and dairy products,…
Q: How does the nucleic acid being purified from associated protein and other impurities?
A: Nucleic acids isolation and purification involve lysis of the cells followed by the removal of…
Q: present briefly the principle of size exclusion chromatography. give a brief description of the use…
A: Here, the particles are separated on the basis of their size. In this chromatography the column is…
Q: Cite the qualitative tests to characterize the products of RNA hydrolysis. Indicate the reagents…
A: The hydrolysis of RNA means it will be broken down,to its native backbone forming structure
Q: A number of tests are used to identify a bacterial pathogen taken from human patients.Research and…
A: The detection of particular antibodies in combination with the evaluation of clinical symptoms or…
Q: Match the following methods of Anlaysis v Fractional analysis, methylation, and periodate oxidation…
A: Different chemical, physical and enzymatic methods in several applications of biological, chemical…
Q: Explain. What is the importance of purifying proteins?
A: Protein purification is the process by which the desired protein is isolated from a sample mixture.…
Q: 9. Describe two biochemical tests in which you could help identify a bacterium in the lab. In your…
A: Biochemical tests are basically the tests that is used to identify bacterial species based on the…
1. Explain the Biochemical method to identify E.Coli
Step by step
Solved in 2 steps
- Briefly discuss 2 fluorogenic methods that can be used in detecting coliforms and E.coli. Include the principle and positive results1. Give the importance of carbohydrate fermentation test in biochemical testing and enumerate the enzymes that are involved. (5 points) 2. What are the substances added in the culture media utilized by the organism producing hydrogen sulfide? Give 2 other media used for the production of hydrogen sulfide. (5 points) 3. Explain the mechanism taking place in the hydrolysis of urea which leads to the formation of bluish red color. ( 5 points) 4. Discuss why human blood plasma will not always yield reliable results. (5 points)present briefly the principle of size exclusion chromatography. give a brief description of the use of protein assays.
- 1. What is the purpose of the different reagents used in the procedure of kato-katz technique? 2. How are the Kato Katz Technique results reported? 3. What are the advantages and disadvantages of using Kato-Katz Technique?1. . The obligate anaerobic bacterium Clostridium perfringens dies when exposed to air. What would predict as the result if you performed a catalase test on this bacterial species? Explain why.2. Complete the table below: Compound tested Which organic molecule was the compound identified as Which reagent helped with the identification of the compound a) Albumin b) Glycine c) Glucose d) Starch e) Sucrose f) Sunflower oil
- Koch's postulates include all of the following EXCEPT O 1) Isolate the organism in pure culture. The symptoms of the test animal may vary from the symptoms seen in the O 2) patient. O 3) Inoculate a test animal with the isolated organism. 4) The organism must be isolated from the test animal in pure culture. 5) The organism isolated from the test animal must match the organism isolated from the patient.Give 2 fluorogenic methods that can be used in detecting coliforms and E.coli.20. Starting with the MacConkey agar and IMVIC tests. Describe how a bacterium can be biochemically identified as Escherichia coli.