7. You are studying a reaction involving o-nitrophenol and want to keep absorption values between 0.2 and 0.8 at 410 nm. What are the lower and upper concentration limits permitted? Concentration (M) Concentration (µM) Lower 3 3 Upper 3 8. You are provided with an NADH solution which you are told was a 150-fold dilution from a stock solution. You record the absorbance of your solution as 0.611 at 340 nm. What was the stock concentration? Stock concentration (M) 3 Stock concentration (mM) 3

7. You are studying a reaction involving o-nitrophenol and want to keep absorption values between 0.2 and 0.8 at 410 nm. What are the lower and upper concentration limits permitted? Concentration (M) Concentration (µM) Lower 3 3 Upper 3 8. You are provided with an NADH solution which you are told was a 150-fold dilution from a stock solution. You record the absorbance of your solution as 0.611 at 340 nm. What was the stock concentration? Stock concentration (M) 3 Stock concentration (mM) 3

Organic Chemistry

8th Edition

ISBN:9781305580350

Author:William H. Brown, Brent L. Iverson, Eric Anslyn, Christopher S. Foote

Publisher:William H. Brown, Brent L. Iverson, Eric Anslyn, Christopher S. Foote

Chapter20: Dienes, Conjugated Systems, And Pericyclic Reactions

Section20.3: Uv-visible Spectroscopy

Problem 20.5P

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