7. You are studying a reaction involving o-nitrophenol and want to keep absorption values between 0.2 and 0.8 at 410 nm. What are the lower and upper concentration limits permitted? Concentration (M) Concentration (µM) Lower 3 3 Upper 3 8. You are provided with an NADH solution which you are told was a 150-fold dilution from a stock solution. You record the absorbance of your solution as 0.611 at 340 nm. What was the stock concentration? Stock concentration (M) 3 Stock concentration (mM) 3

Organic Chemistry
8th Edition
ISBN:9781305580350
Author:William H. Brown, Brent L. Iverson, Eric Anslyn, Christopher S. Foote
Publisher:William H. Brown, Brent L. Iverson, Eric Anslyn, Christopher S. Foote
Chapter20: Dienes, Conjugated Systems, And Pericyclic Reactions
Section20.3: Uv-visible Spectroscopy
Problem 20.5P
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7. You are studying a reaction involving o-nitrophenol and want to keep absorption values between 0.2 and 0.8 at 410 nm. What are
the lower and upper concentration limits permitted?
Concentration (M)
Concentration (µM)
Lower
3
3
Upper
3
8. You are provided with an NADH solution which you are told was a 150-fold dilution from a stock solution. You record the
absorbance of your solution as 0.611 at 340 nm. What was the stock concentration?
Stock concentration (M)
3
Stock concentration (mM)
3
Transcribed Image Text:7. You are studying a reaction involving o-nitrophenol and want to keep absorption values between 0.2 and 0.8 at 410 nm. What are the lower and upper concentration limits permitted? Concentration (M) Concentration (µM) Lower 3 3 Upper 3 8. You are provided with an NADH solution which you are told was a 150-fold dilution from a stock solution. You record the absorbance of your solution as 0.611 at 340 nm. What was the stock concentration? Stock concentration (M) 3 Stock concentration (mM) 3
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